PDE6 beta Antibody

Images

 
Western Blot: PDE6 beta Antibody [NB120-5663] - Analysis of PDE6 beta. Lane 1 demostrates no detection on extracts of RD mice. Lane 2 shows a single band at ~90 kDa on Balb/C extracts.
Immunocytochemistry/ Immunofluorescence: PDE6 beta Antibody [NB120-5663] - Mouse retina outer segments.
Immunohistochemistry-Frozen: PDE6 beta Antibody [NB120-5663] - Specific labeling of the photoreceptor outer segments with minimal background. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Bv, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PDE6 beta Antibody Summary

Immunogen
Synthetic Peptides: H(20) Q Y F G (K/R) K L S P E N V A G A C(36)
Localization
Plasma membrane
Specificity
Detects PDE6B from mouse retinal extracts.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDE6B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
WB: Detects an approx. 90 kDa protein.
Reviewed Applications
Read 1 Review rated 4
using
NB120-5663 in the following applications:

Publications
Read Publication using NB120-5663.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS containing 1mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDE6 beta Antibody

  • CSNB3
  • EC 3.1.4
  • EC 3.1.4.35
  • GMP-PDE beta
  • PDEBrod cGMP-phosphodiesterase beta-subunit
  • phosphodiesterase 6B, cGMP-specific, rod, beta
  • rd1
  • rod cGMP-specific 3'-5'-cyclic phosphodiesterase subunit beta
  • RP40CSNBAD2

Background

Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDE6 beta Antibody (NB120-5663)(1)

We have publications tested in 1 confirmed species: Human.


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Review for PDE6 beta Antibody (NB120-5663) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NB120-5663:
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IHC-Fr
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen PDE6 beta NB120-5663
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reviewed by:
Freya Mowat
IHC-Fr Other 03/24/2014
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedCanine Retina
SpeciesOther

Primary Anitbody

Dilution Ratio1:250 4C overnight

Details

Fixation Details4% PFA fixed tissue

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDE6 beta Antibody (NB120-5663) (0)

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Secondary Antibodies

 

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Recent Reviews

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5
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4
1
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0
2
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1
0

Freya Mowat
03/24/2014
Application: IHC-Fr
Species: Other

Bioinformatics

Gene Symbol PDE6B
Entrez
Uniprot