LEF1 Antibody (S02-3A4)

Western Blot: LEF1 Antibody (S02-3A4) [NBP3-19675] - Western blot detection of LEF1 in Jurkat cell lysates using NBP3-19675 (1:1000 diluted). Predicted band size: 44kDa. Observed band size: 25-58kDa.

• Reactivity: Hu
• Applications: WB
• Clone: S02-3A4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

LEF1 Antibody (S02-3A4) Summary

• Description: Novus Biologicals Rabbit LEF1 Antibody (S02-3A4) (NBP3-19675) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human LEF1 (Uniprot # Q9UJU2)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LEF1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for LEF1 Antibody (S02-3A4)

• DKFZp586H0919
• FLJ46390
• LEF1
• LEF-1
• lymphoid enhancer-binding factor 1
• T cell-specific transcription factor 1-alpha
• TCF10
• TCF1ALPHA
• TCF1-alpha
• TCF7L3

Background

Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LEF1