Lck Antibody (SR2027) - BSA Free

Western Blot: Lck Antibody (SR2027) [NBP3-21829] - Western blot analysis using NBP3-21829 in Jurkat cell lysate.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: SR2027
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lck Antibody (SR2027) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Lck (Uniprot #: P06239)
• Specificity: Detects endogenous levels of total Lck
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LCK
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:80
  • Western Blot 1:500-1:1000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Lck Antibody (SR2027) - BSA Free

• EC 2.7.10
• EC 2.7.10.2
• Hck-3
• Lck
• Lcktkr
• Leukocyte C-terminal Src kinase
• LSK
• LSTRA
• Lymphocyte cell-specific protein-tyrosine kinase
• lymphocyte-specific protein tyrosine kinase
• p56(LSTRA) protein-tyrosine kinase
• p56lck
• p56-LCK
• pp58lck
• Protein YT16
• Proto-oncogene Lck
• proto-oncogene tyrosine-protein kinase LCK
• T cell-specific protein-tyrosine kinase
• T-lymphocyte specific protein tyrosine kinase p56lck
• tyrosine-protein kinase Lck
• YT16

Background

The human T-cell or lymphocyte specific gene Lck/p56 is a member of the Src family of non-receptors tyrosine kinase. Mostly expressed in T cells, Lck has been shown to be critical for the normal development of T lymphocytes (1). Additionally, the N-terminal region of the tyrosine kinase Lck interacts with the cytoplasmic domains of CD4 and CD8 (2). Autophosphorylation at Tyr-394 appears to be required for maximum catalytic activity (3) but it can also be suppressed by phosphorylation of the carboxyl-terminal tyrosine Tyr-505 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LCK