Lck Antibody (LCK-01)

Images

 
Western Blot: Lck Antibody (LCK-01) [NB500-336] - Fig. 1. Western Blotting analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01). Lane 1: J. CaM-1.6 cell line (a mutant derivate ...read more

Product Details

Summary
Reactivity Hu, Mu(-)Species Glossary
Applications WB, Flow, ICC/IF, IP, CyTOF-ready
Clone
LCK-01
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Lck Antibody (LCK-01) Summary

Immunogen
Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
Localization
Cytoplasmic
Specificity
The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LCK
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • CyTOF-ready
Positive Control
Jurkat Lysate (NB800-PC2)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Lck Antibody (LCK-01)

  • EC 2.7.10
  • EC 2.7.10.2
  • Hck-3
  • Lck
  • Lcktkr
  • Leukocyte C-terminal Src kinase
  • LSK
  • LSTRA
  • Lymphocyte cell-specific protein-tyrosine kinase
  • lymphocyte-specific protein tyrosine kinase
  • p56(LSTRA) protein-tyrosine kinase
  • p56lck
  • p56-LCK
  • pp58lck
  • Protein YT16
  • Proto-oncogene Lck
  • proto-oncogene tyrosine-protein kinase LCK
  • T cell-specific protein-tyrosine kinase
  • T-lymphocyte specific protein tyrosine kinase p56lck
  • tyrosine-protein kinase Lck
  • YT16

Background

Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lck Antibody (NB500-336) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-336AF405
Alexa Fluor 488 Labeled NB500-336AF488
Alexa Fluor 647 Labeled NB500-336AF647
Alexa Fluor 700 Labeled NB500-336AF700
Allophycocyanin Labeled NB500-336APC
DyLight 350 Labeled NB500-336UV
DyLight 405 Labeled NB500-336V
DyLight 488 Labeled NB500-336G
DyLight 680 Labeled NB500-336FR
DyLight 755 Labeled NB500-336IR
PE Labeled NB500-336PE
PerCP Labeled NB500-336PCP

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Bioinformatics Tool for Lck Antibody (NB500-336)

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Bioinformatics

Gene Symbol LCK
Entrez