Lck Antibody (33D196)

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Western Blot: Lck Antibody (33D196) [NB100-56678] - Western blot analysis for Lck using NB100-56678 at 1:1000 dilution against 20 ug/lane of Jurkat cell lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
33D196
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Lck Antibody (33D196) Summary

Immunogen
This monoclonal antibody was raised against a synthetic peptide containing amino acid residues of human lck protein.
Specificity
Lck (33D196)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LCK
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Positive Control
Jurkat Lysate (NB800-PC2)

Reactivity Notes

Reacts with human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Lck Antibody (33D196)

  • EC 2.7.10
  • EC 2.7.10.2
  • Hck-3
  • Lck
  • Lcktkr
  • Leukocyte C-terminal Src kinase
  • LSK
  • LSTRA
  • Lymphocyte cell-specific protein-tyrosine kinase
  • lymphocyte-specific protein tyrosine kinase
  • p56(LSTRA) protein-tyrosine kinase
  • p56lck
  • p56-LCK
  • pp58lck
  • Protein YT16
  • Proto-oncogene Lck
  • proto-oncogene tyrosine-protein kinase LCK
  • T cell-specific protein-tyrosine kinase
  • T-lymphocyte specific protein tyrosine kinase p56lck
  • tyrosine-protein kinase Lck
  • YT16

Background

Lck is a lymphoid-specific cytosolic protein tyrosine kinase (PTK), which is essential for T-cell development and function. It is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAMs) in the cytoplasmic tails of the TCR-chains and CD3 subunits. The phospho-ITAMs serve as docking sites for Src homology domain 2 (SH2)-containing molecules, predominantly ZAP-70 and Syk. Only then can ZAP-70 undergo tyrosine phosphorylation, become enzymatically active and further phosphorylate downstream effector molecules. In addition, Lck contributes to signaling by other receptor molecules. Lck is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature TCR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lck Antibody (NB100-56678). (Showing 1 - 1 of 1 FAQ).

  1. Do you have the immunogen sequence for the LCK antibody (clone 33D196)?
    • The immunogen site lies with in amino acids 360-410.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56678AF405
Alexa Fluor 488 Labeled NB100-56678AF488
Alexa Fluor 647 Labeled NB100-56678AF647
Alexa Fluor 700 Labeled NB100-56678AF700
Biotin Labeled NB100-56678B
DyLight 350 Labeled NB100-56678UV
DyLight 405 Labeled NB100-56678V
DyLight 488 Labeled NB100-56678G
DyLight 550 Labeled NB100-56678R
DyLight 650 Labeled NB100-56678C
DyLight 680 Labeled NB100-56678FR
DyLight 755 Labeled NB100-56678IR
HRP Labeled NB100-56678H

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Bioinformatics Tool for Lck Antibody (NB100-56678)

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Bioinformatics

Gene Symbol LCK
Entrez
Uniprot