LC3B Antibody (1251B) [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, KO
• Clone: 1251B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 750

LC3B Antibody (1251B) [Alexa Fluor® 750] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human LC3B synthetic peptide
  Accession # Q9GZQ8
• Specificity: Detects human, mouse, and rat LC3B in Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LC3B Antibody (1251B) [Alexa Fluor® 750]

• Apg8b
• ATG8F
• Autophagy-related protein LC3 B
• Autophagy-related ubiquitin-like modifier LC3 B
• lc3b autophagy marker
• LC3B
• lc3-i, ii
• LC3II
• MAP1 light chain 3-like protein 2
• MAP1A/1BLC3
• MAP1A/MAP1B LC3 B
• MAP1LC3B
• MAP1LC3B-a
• microtubule associated protein 1 light chain 3 beta
• microtubule associated protein 3 b
• microtubule associated protein 3 beta
• microtubule-associated proteins 1A/1B light chain 3
• microtubule-associated proteins 1A/1B light chain 3B

Background

Human Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of Autophagy-related 8 (Atg8) proteins (1). The LC3 subfamily also includes LC3B andLC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins (3). However, LC3 has gained attention for MAP1-independent functions in autophagy. LC3 utilizes a ubiquitin-like conjugation system that includes E1-, E2-, and E3-like enzymes to covalently attach phosphatidylethanolamine (PE) to its C-terminus, incorporating it into the phagophore membrane during the early stages of autophagasome formation (4). Recruitment of LC3 to the phagophore may promote membrane elongation (4,5). It may also be involved in cargo recruitment to autophagosomes (1). LC3 is often used as a marker of autophagy. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for LC3B Antibody (FAB85582S). (Showing 1 - 1 of 1 FAQ).

1. Hello, somewhere on the LC3B antibody page I read it is recommended to use a 0.2uM membrane for western blot?
   • Yes, because LC3B-I is about 14-15 kDa and LC3B-II is even even smaller, the protein may slip through the 0.45 uM membrane and be lost during blotting. However, there has been success using 0.45 uM membranes; a 0.2 uM membrane is something to consider if no signal is detected.