LAT [p Tyr191] Antibody - Azide and BSA Free

Western Blot: LAT [p Tyr191] Antibody [NBP3-16940] - Western blot analysis of extracts of various cell lines, using LAT antibody (NBP3-16940) at 1:1000 dilution.Jurkat cells were treated by CD-3 (10 ug/mL) at 37C for 2 minutes after serum-starvation overnight.NIH/3T3 cells were treated by 10% FBS at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LAT [p Tyr191] Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit LAT [p Tyr191] Antibody - Azide and BSA Free (NBP3-16940) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around Y191 of human LAT (NP_001014987.1). REYVN
• Modification: p Tyr191
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LAT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for LAT [p Tyr191] Antibody - Azide and BSA Free

• 36 kDa phospho-tyrosine adapter protein
• LAT
• LAT1,36 kDa phospho-tyrosine adaptor protein
• linker for activation of T cells
• linker for activation of T-cells family member 1
• p36-38
• pp36

Background

LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: LAT