LAT Antibody (SR1593) - BSA Free

Western Blot: LAT Antibody (SR1593) [NBP3-22544] - Western blot analysis using NBP3-22544 in Jurkat cell lysate.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: SR1593
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LAT Antibody (SR1593) - BSA Free Summary

• Description: Novus Biologicals Rabbit LAT Antibody (SR1593) - BSA Free (NBP3-22544) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human LAT (Uniprot #: O43561)
• Specificity: Detects endogenous levels of total LAT
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LAT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for LAT Antibody (SR1593) - BSA Free

• 36 kDa phospho-tyrosine adapter protein
• LAT
• LAT1,36 kDa phospho-tyrosine adaptor protein
• linker for activation of T cells
• linker for activation of T-cells family member 1
• p36-38
• pp36

Background

LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAT