LAP (TGF-beta 1) Antibody [Unconjugated]

Images

 
Western blot shows lysates of Saos‑2 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog #AF-246-NA) followed ...read more
Recombinant Human LAP TGF‑ beta 1 (Catalog # 246‑LP) inhibits Recom­binant Human TGF‑ beta 1 (Catalog # 240‑B) growth inhibition activity in the HT‑2 mouse T cell line in a dose-dependent manner (orange ...read more
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LAP (TGF-beta 1) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

LAP (TGF-beta 1) Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived and Chinese hamster ovary cell line CHO-derived recombinant human LAP TGF‑ beta 1
Leu30-Ser390
Accession # P01137
Specificity
Detects human LAP TGF‑ beta 1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFB1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Simple Western 20 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 0.4-2 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF-246-NA in the following applications:

Publications
Read Publications using
AF-246-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAP (TGF-beta 1) Antibody [Unconjugated]

  • CED
  • DPD1
  • LAP (TGFbeta 1)
  • LAP (TGF-beta 1)
  • LAP
  • TGFB
  • TGFB1
  • TGFbeta
  • transforming growth factor beta 1

Background

TGF-beta 1 (transforming growth factor beta 1) and the closely related TGF-beta 2 and -beta 3 are members of the large TGF-beta  superfamily. TGF‑ beta proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑3). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (4). A furin-like convertase processes the proprotein within the trans-Golgi to generate an N‑terminal 249 aa (aa 30-278) latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-beta 1 (aa 279-390) (4‑6). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non‑covalently associated after secretion, forming the small latent TGF-beta 1 complex (4‑8). Purified LAP is also capable of associating with active TGF‑ beta with high affinity, and can neutralize TGF-beta activity (9). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (5-7). TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins (5, 8). The LAP portion of human TGF-beta 1 shares 91%, 92%, 85%, 86% and 88% aa identity with porcine, canine, mouse, rat and equine TGF-beta 1 LAP, respectively, while mature human TGF-beta 1 portion shares 100% aa identity with porcine, canine and bovine TGF-beta 1, and 99% aa identity with mouse, rat and equine TGF-beta 1. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species (9). Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF‑ beta 1 (10).

  1. Dunker, N. & K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  2. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  3. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  4. Derynck, R. et al. (1985) Nature 316:701.
  5. Dabovic, B. and D.B. Rifkin (2008) “TGF-beta Bioavailability” in The TGF-beta Family. Derynck, R. and K. Miyazono (eds): Cold Spring Harbor Laboratory Press, p. 179.
  6. Brunner, A.M. et al. (1989) J. Biol. Chem. 264:13660.
  7. Miyazono, K. et al. (1991) EMBO J. 10:1091.
  8. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  9. Miller, D.M. et al. (1992) Mol. Endocrinol. 6:694.
  10. Janssens, K. et al. (2003) J. Biol. Chem. 278:7718.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAP (TGF-beta 1) Antibody (AF-246-NA)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: ICC, IHC, IHC Frozen, IHC Paraffin-embedded, Neut, WB.


Filter By Application
ICC
(1)
IHC
(2)
IHC Frozen
(1)
IHC Paraffin-embedded
(1)
Neut
(5)
WB
(5)
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Filter By Species
Human
(10)
Mouse
(3)
Rat
(1)
Porcine
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF-246-NA Applications Species
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC,, Mouse) IHC Mouse
E Ventura, M Weller, I Burghardt Cutting Edge: ERK1 Mediates the Autocrine Positive Feedback Loop of TGF-? and Furin in Glioma-Initiating Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28484053] (WB,, Human) WB Human
O Miroshnych, WT Chang, JL Dragoo The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration Am J Sports Med, 2017;45(4):945-953. 2017 [PMID: 28027451] (WB,, Human) WB Human
Boris Hinz MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells Nat Mater, 2016;0(0):. 2016 [PMID: 27798620] (WB,, Rat) WB Rat
Yasmin N, Bauer T, Modak M, Wagner K, Schuster C, Koffel R, Seyerl M, Stockl J, Elbe-Burger A, Graf D, Strobl H Identification of bone morphogenetic protein 7 (BMP7) as an instructive factor for human epidermal Langerhans cell differentiation. J Exp Med, 2013;210(12):2597-610. 2013 [PMID: 24190429] (IHC Frozen,, Human) IHC Frozen Human
Shahinian H, Loessner D, Biniossek M, Kizhakkedathu J, Clements J, Magdolen V, Schilling O Secretome and degradome profiling shows that Kallikrein-related peptidases 4, 5, 6, and 7 induce TGFbeta-1 signaling in ovarian cancer cells. Mol Oncol, 2014;8(1):68-82. 2014 [PMID: 24120346] (WB,, Human) WB Human
Shinojima N, Hossain A, Takezaki T, Fueyo J, Gumin J, Gao F, Nwajei F, Marini F, Andreeff M, Kuratsu J, Lang F TGF-beta mediates homing of bone marrow-derived human mesenchymal stem cells to glioma stem cells. Cancer Res, 2013;73(7):2333-44. 2013 [PMID: 23365134] (Neut,, Human) Neut Human
Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, Nakauchi H Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche. Cell, 2011;147(5):1146-58. 2011 [PMID: 22118468] (ICC,, Mouse) ICC Mouse
Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31. Exp. Cell Res., 2011;317(7):976-93. 2011 [PMID: 21182835] (WB,, Human) WB Human
Ha CT, Wu JA, Irmak S, Lisboa FA, Dizon AM, Warren JW, Ergun S, Dveksler GS Human pregnancy specific beta-1-glycoprotein 1 (PSG1) has a potential role in placental vascular morphogenesis. Biol. Reprod., 2010;83(1):27-35. 2010 [PMID: 20335639] (Neut,, Human) Neut Human
Show All 15 Publications.

Reviews for LAP (TGF-beta 1) Antibody (AF-246-NA) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Other.

Reviews using AF-246-NA:
Filter by Applications
WB
(2)
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Filter by Species
Other
(2)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Other 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21998117
SpeciesOther

Blocking

Blocking DetailsSee PMID 21998117

Details

Detection NotesSee PMID 21998117

Comments

CommentsPublished: See PMID 21998117
 
reviewed by:
Anonymous
WB Other 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22835852
SpeciesOther

Blocking

Blocking DetailsSee PMID 22835852

Details

Detection NotesSee PMID 22835852

Comments

CommentsPublished: See PMID 22835852Cross reacts with mouse and hamster LAP

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Anonymous
01/07/2015
Application: WB
Species: Other

 
Anonymous
01/07/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol TGFB1
Entrez
Uniprot