LAP (TGF-beta 1) Antibody (27232) [Unconjugated]

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 27232
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

LAP (TGF-beta 1) Antibody (27232) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
  Leu30-Arg278 (Cys33Ser)
  Accession # P01137
• Specificity: Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta 1, rhTGF‑ beta 2, rhTGF‑ beta 1.2, rhTGF‑ beta 3, or rhTGF‑ alpha .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAP (TGF-beta 1) Antibody (27232) [Unconjugated]

• CED
• DPD1
• LAP (TGFbeta 1)
• LAP (TGF-beta 1)
• LAP
• TGFB
• TGFB1
• TGFbeta
• transforming growth factor beta 1

Background

The TGF‑ beta family includes several related proteins (70‑80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF‑ beta 1, TGF‑ beta 2, TGF‑ beta 1.2, TGF‑ beta 3, TGF‑ beta 4, and TGF‑ beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta , that consists of TGF‑ beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF‑ beta family members are naturally associated with their own distinct LAPs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAP (TGF-beta 1) Antibody (MAB2463)(10)

Publications using MAB2463

• Sabrina, S;Takeda, Y;Kato, T;Naito, S;Ito, H;Takai, Y;Ushijima, M;Narisawa, T;Kanno, H;Sakurai, T;Saitoh, S;Araki, A;Tsuchiya, N;Asao, H; Initial Myeloid Cell Status Is Associated with Clinical Outcomes of Renal Cell Carcinoma Biomedicines 2023-04-27 [PMID: 37238964] (Flow Cytometry, Human)
• JH Jeong, SJ Choi, SM Ahn, JS Oh, YG Kim, CK Lee, B Yoo, S Hong Neutrophil extracellular trap clearance by synovial macrophages in gout Arthritis Research & Therapy, 2021-03-19;23(1):88. 2021-03-19 [PMID: 33741037] (Flow Cytometry, Human)
• T Tadmor, I Levy, Z Vadasz Hierarchical involvement of myeloid derived suppressor cells and monocytes expressing latency-associated peptide in plasma cell dyscrasias Turk J Haematol, 2018-03-28;0(0):. 2018-03-28 [PMID: 29589834] (Flow Cytometry, Human)
• K Trojan, L Zhu, M Aly, R Weimer, N Bulut, C Morath, G Opelz, V Daniel Association of peripheral NK cell counts with Helios+IFNy- Tregs in patients with good long-term renal allograft function Clin. Exp. Immunol, 2017-03-13;0(0):. 2017-03-13 [PMID: 28194759] (Flow Cytometry, Human)
• Nieminen J, Sipponen T, Farkkila M, Vaarala O Monocyte-derived dendritic cells from Crohn's disease patients exhibit decreased ability to activate T helper type 17 responses in memory cells. Clin Exp Immunol, 2014-07-01;177(1):190-202. 2014-07-01 [PMID: 24635023] (Flow Cytometry, Human)
• Boswell S, Sharif S, Alisa A, Pereira SP, Williams R, Behboudi S Induction of latency-associated peptide (transforming growth factor-beta(1)) expression on CD4+ T cells reduces Toll-like receptor 4 ligand-induced tumour necrosis factor-alpha production in a transforming growth factor-beta-dependent manner. Immunology, 2011-03-23;133(3):278-87. 2011-03-23 [PMID: 21426338] (Flow Cytometry, Human)
• Oida T, Weiner HL Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß. J. Immunol., 2010-08-18;185(6):3529-35. 2010-08-18 [PMID: 20720212] (Flow Cytometry, Human)
• Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells. J. Exp. Med., 2009-01-12;206(1):169-81. 2009-01-12 [PMID: 19139172] (IHC-Fr, Human)
• Savage ND, De Boer T, Walburg KV, Joosten SA, van Meijgaarden K, Geluk A, Ottenhoff TH Human anti-inflammatory macrophages induce Foxp3+ GITR+ CD25+ regulatory T cells, which suppress via membrane-bound TGFbeta-1. J. Immunol., 2008-08-01;181(3):2220-6. 2008-08-01 [PMID: 18641362] (Flow Cytometry, Human)
• Kitani A, Fuss I, Pedersen A, Nawata H TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice. J. Immunol., 2004-01-15;172(2):834-42. 2004-01-15 [PMID: 14707053] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TGFB1
• Uniprot:
  • P01137(Human)