LAP (TGF-beta 1) Antibody (27232) [Allophycocyanin]

Human platelets were stained with Mouse Anti-Human LAP (TGF-beta 1) APC-conjugated Monoclonal Antibody (Catalog # FAB2463A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 27232
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

LAP (TGF-beta 1) Antibody (27232) [Allophycocyanin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
  Leu30-Arg278 (Cys33Ser)
  Accession # P01137
• Specificity: Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta 1, rhTGF‑ beta 2, rhTGF‑ beta 1.2, rhTGF‑ beta 3, or rhTGF‑ alpha .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGFB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAP (TGF-beta 1) Antibody (27232) [Allophycocyanin]

• CED
• DPD1
• LAP (TGFbeta 1)
• LAP (TGF-beta 1)
• LAP
• TGFB
• TGFB1
• TGFbeta
• transforming growth factor beta 1

Background

The TGF‑ beta family includes several related proteins (70‑80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF‑ beta 1, TGF‑ beta 2, TGF‑ beta 1.2, TGF‑ beta 3, TGF‑ beta 4, and TGF‑ beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta , that consists of TGF‑ beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF‑ beta family members are naturally associated with their own distinct LAPs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LAP (TGF-beta 1) Antibody (FAB2463A)(4)

Publications using FAB2463A

• T Kato, Y Takeda, H Ito, Y Kurota, A Yamagishi, T Sakurai, S Naito, A Araki, H Nara, H Asao, N Tsuchiya GPI-80 as a Useful Index for Myeloid Cell Heterogeneity and a Potential Prognostic Biomarker for Metastatic Renal Cell Carcinoma Tohoku J. Exp. Med., 2019-11-01;249(3):203-212. 2019-11-01 [PMID: 31776298] (Flow Cytometry, Human)
• Huygens C, Lienart S, Dedobbeleer O, Stockis J, Gauthy E, Coulie P, Lucas S Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor beta1 (TGF-beta1) Production in Human Regulatory T Cells. J Biol Chem, 2015-06-30;290(33):20105-16. 2015-06-30 [PMID: 26126825] (Flow Cytometry, Human)
• Zhu Z, Meng K, Zhong Y, Qi L, Mao X, Yu K, Zhang W, Zhu P, Ren Z, Wu B, Ji Q, Wang X, Zeng Q Impaired circulating CD4+ LAP+ regulatory T cells in patients with acute coronary syndrome and its mechanistic study. PLoS ONE, 2014-02-18;9(2):e88775. 2014-02-18 [PMID: 24558424] (Flow Cytometry, Human)
• Radulovic K, Manta C, Rossini V, Holzmann K, Kestler HA, Wegenka UM, Nakayama T, Niess JH CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential. J. Immunol., 2012-01-16;188(4):2001-13. 2012-01-16 [PMID: 22250092] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TGFB1
• Uniprot:
  • P01137(Human)