| Reactivity | HuSpecies Glossary |
| Applications | Flow |
| Clone | 27232 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alexa Fluor 350 |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | FAB2463V-100UG | |||
| Alexa Fluor 488 | FAB2463G | |||
| Alexa Fluor 594 | FAB2463T-100UG | |||
| Alexa Fluor 647 | FAB2463R-100UG | |||
| Alexa Fluor 700 | FAB2463N | |||
| Alexa Fluor 750 | FAB2463S-100UG | |||
| Allophycocyanin | FAB2463A | |||
| Phycoerythrin | FAB2463P | |||
| Unconjugated | MAB2463 | |||
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1) Leu30-Arg278 (Cys33Ser) Accession # P01137 |
| Specificity | Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta 1, rhTGF‑ beta 2, rhTGF‑ beta 1.2, rhTGF‑ beta 3, or rhTGF‑ alpha. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TGFB1 |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
| Preservative | 0.09% Sodium Azide |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
The TGF‑ beta family includes several related proteins (70‑80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF‑ beta 1, TGF‑ beta 2, TGF‑ beta 1.2, TGF‑ beta 3, TGF‑ beta 4, and TGF‑ beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF‑ beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF‑ beta family members are naturally associated with their own distinct LAPs.
![LAP (TGF-beta 1) Antibody (27232) [Alexa Fluor® 350]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "LAP (TGF-beta 1) Antibody (27232) [Alexa Fluor® 350]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | FAB2463U | |
| Alexa Fluor 405 | FAB2463V | |
| Alexa Fluor 488 | FAB2463G | |
| Alexa Fluor 594 | FAB2463T | |
| Alexa Fluor 647 | FAB2463R | |
| Alexa Fluor 700 | FAB2463N | |
| Alexa Fluor 750 | FAB2463S | |
| Allophycocyanin | FAB2463A | |
| Phycoerythrin | FAB2463P |
Diseases for LAP (TGF-beta 1) Antibody (FAB2463U)Discover more about diseases related to LAP (TGF-beta 1) Antibody (FAB2463U).
| Pathways for LAP (TGF-beta 1) Antibody (FAB2463U)View related products by pathway.
|
PTMs for LAP (TGF-beta 1) Antibody (FAB2463U)Learn more about PTMs related to LAP (TGF-beta 1) Antibody (FAB2463U).
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![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
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