Langerin/CD207 Antibody (DCGM4/122D5) - Azide and BSA Free

Western Blot: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Western-blot DCGM4 1: non-reduced 2: reduced

Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - lung tumor tissue section

Flow Cytometry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100]

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Analysis of Alexa Fluor (R) 546 conjugate of DDX0363P-100. Staining of human skin frozen section.

Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - CHO control

Immunohistochemistry: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin-transfected CHO

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin co-localizes with caveolin-1 in steady state. Confocal scanning laser microscopic analyses for THP-langerin cell line (A), MUTZ3-LC cell line (MUTZ-LCs, B) & human LCs (C) for langerin & caveolin-1 in steady-state condition in permeablized cells; bars represent 10 μm (A, B, C). Langerin & caveolin-1 were immuno-precipitated from LC cell lysates & immuno-blotted with antibodies against caveolin-1 or langerin (D). These data are representative of at least 3 donors or 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25551286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin co-localizes with caveolin-1 in steady state. Confocal scanning laser microscopic analyses for THP-langerin cell line (A), MUTZ3-LC cell line (MUTZ-LCs, B) & human LCs (C) for langerin & caveolin-1 in steady-state condition in permeablized cells; bars represent 10 μm (A, B, C). Langerin & caveolin-1 were immuno-precipitated from LC cell lysates & immuno-blotted with antibodies against caveolin-1 or langerin (D). These data are representative of at least 3 donors or 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25551286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin co-localizes with caveolin-1 in steady state. Confocal scanning laser microscopic analyses for THP-langerin cell line (A), MUTZ3-LC cell line (MUTZ-LCs, B) & human LCs (C) for langerin & caveolin-1 in steady-state condition in permeablized cells; bars represent 10 μm (A, B, C). Langerin & caveolin-1 were immuno-precipitated from LC cell lysates & immuno-blotted with antibodies against caveolin-1 or langerin (D). These data are representative of at least 3 donors or 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25551286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (DCGM4/122D5) [DDX0363P-100] - Langerin co-localizes with caveolin-1 in steady state. Confocal scanning laser microscopic analyses for THP-langerin cell line (A), MUTZ3-LC cell line (MUTZ-LCs, B) & human LCs (C) for langerin & caveolin-1 in steady-state condition in permeablized cells; bars represent 10 μm (A, B, C). Langerin & caveolin-1 were immuno-precipitated from LC cell lysates & immuno-blotted with antibodies against caveolin-1 or langerin (D). These data are representative of at least 3 donors or 3 independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25551286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Ca
• Applications: WB, Flow, ICC/IF, IHC, Binding Activity
• Clone: DCGM4/122D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Langerin/CD207 Antibody (DCGM4/122D5) - Azide and BSA Free Summary

• Immunogen: Langerin/CD207 was used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD207
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30223113).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Langerin/CD207 Antibody (DCGM4/122D5) - Azide and BSA Free

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• Langerin

Background

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Langerin/CD207 Antibody (DDX0363P-100)(47)

Publications using DDX0363P-100

• Jackson R, Lukacs JD, Zehbe I. The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans Cells Viruses 2020-12-01 [PMID: 33271909] (Flow Cytometry, Human)
• Del Duca E, Pavel AB, Dubin C et al. Major Differences in Expression of Inflammatory Pathways in Skin from Different Body Sites of Healthy Individuals J. Invest. Dermatol. 2019-10-01 [PMID: 31059695] (IF/IHC, Human)
• Sanyal RD, Pavel AB, Glickman J et al. Atopic dermatitis in African American patients is TH2/TH22-skewed with TH1/TH17 attenuation Ann. Allergy Asthma Immunol. 2019-01-01 [PMID: 30223113] (ICC/IF, Human)
• Reinartz et al. Dendritic Cell Subsets in Oral Mucosa of Allergic and Healthy Subjects. PLoS One 2016-05-11 [PMID: 27166951]
• Fehres CM et al. Phenotypic and Functional Properties of Human Steady State CD14+ and CD1a+ Antigen Presenting Cells and Epidermal Langerhans Cells. PLoS One 2015-11-25 [PMID: 26605924]
• Ng WC et al. The C-type Lectin Langerin Functions as a Receptor for Attachment and Infectious Entry of Influenza A Virus. J Virol 2015-10-14 [PMID: 26468543]
• Van den Berg LM et al. Caveolin-1 mediated uptake via langerin restricts HIV-1 infection in human Langerhans cells. Retrovirology 2014-12-31 [PMID: 25551286]
• Schaeffer E et al. Dermal CD14(+) Dendritic Cell and Macrophage Infection by Dengue Virus Is Stimulated by Interleukin-4. J Invest Dermatol 2015-01-01 [PMID: 25521455]
• Bigley V et al. Langerin-expressing dendritic cells in human tissues are related to CD1c+ dendritic cells and distinct from Langerhans cells and CD141high XCR1+ dendritic cells. J Leukoc Biol 2015-04-01 [PMID: 25516751]
• Milne P et al. CD1c+ blood dendritic cells have Langerhans cell potential. Blood 2015-01-15 [PMID: 25352125]
• Stoitzner P et al. Human skin dendritic cells can be targeted in situ by intradermal injection of antibodies against lectin receptors. Exp Dermatol 2014-12-01 [PMID: 25346475]
• Flacher V et al. Murine Langerin+ dermal dendritic cells prime CD8+ T cells while Langerhans cells induce cross-tolerance. EMBO Mol Med 2014-08-01 [PMID: 25085878]
• Nasr N et al. Inhibition of two temporal phases of HIV-1 transfer from primary Langerhans cells to T cells: the role of langerin. J Immunol 2014-09-01 [PMID: 25070850]
• Larbi et al. Generation of multipotent early lymphoid progenitors from human embryonic stem cells. Stem Cells Dev 2014-12-15 [PMID: 24955741]
• Gulati N et al. Molecular characterization of human skin response to diphencyprone at peak and resolution phases: therapeutic insights. J Invest Dermatol 2014-10-01 [PMID: 24751728]
• Stoll Pet al. Impact of smoking on dendritic cell phenotypes in the airway lumen of patients with COPD. Respir Res 2014-04-18 [PMID: 24742278]
• Schuster C et al. Human embryonic epidermis contains a diverse Langerhans cell precursor pool. Development 2014-01-01 [PMID: 24496618]
• Lommatzsch M et al. Fluticasone impact on airway dendritic cells in smokers: a randomized controlled trial. Respir Res 2013-10-29 [PMID: 24168756]
• Debeer S et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol 2013-07-01 [PMID: 24047577]
• Welty NE et al. Intestinal lamina propria dendritic cells maintain T cell homeostasis but do not affect commensalism. J Exp Med 2013-09-23 [PMID: 24019552]
• Bonetti M et al. Rosiglitazone promotes the differentiation of Langerhans cells and inhibits that of other dendritic cell types from CD133 positive hematopoietic precursors. Histol Histopathol 2014-03-01 [PMID: 23881602]
• Tjomsland V et al. Blocking of integrins inhibits HIV-1 infection of human cervical mucosa immune cells with free and complement-opsonized virions. Eur J Immunol 2013-09-01 [PMID: 23686382]
• Luckashenak N et al. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol 2013-01-01 [PMID: 23209325]
• Mayumi N et al. E-cadherin interactions are required for Langerhans cell differentiation. Eur J Immunol 2013-01-01 [PMID: 23135957]
• Hitzler M et al. Human Langerhans cells control Th cells via programmed death-ligand 1 in response to bacterial stimuli and nickel-induced contact allergy. PLoS One 2012-01-01 [PMID: 23056446]
• Iram N et al. Age-related changes in expression and function of Toll-like receptors in human skin. Development 2012-11-01 [PMID: 23034637]
• Haniffa M et al. Human tissues contain CD141hi cross-presenting dendritic cells with functional homology to mouse CD103+ nonlymphoid dendritic cells. Immunity 2012-07-27 [PMID: 22795876]
• Schwingshackl P et al. Distribution and maturation of skin dendritic cell subsets in two forms of cutaneous T-cell lymphoma: mycosis fungoides and Sezary syndrome. Acta Derm Venereol 2012-05-01 [PMID: 22678564]
• Segura E et al. Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med 2012-04-09 [PMID: 22430490]
• van der Vliest M et al. Human Langerhans cells capture measles virus through Langerin and present viral antigens to CD4+ T cells but are incapable of cross-presentation. Eur J Immunol 2011-09-01 [PMID: 21739428]
• Fujita H et al. Lesional dendritic cells in patients with chronic atopic dermatitis and psoriasis exhibit parallel ability to activate T-cell subsets. J Allergy Clin Immunol 2011-09-01 [PMID: 21704361]
• Ouwehand K et al. Technical advance: Langerhans cells derived from a human cell line in a full-thickness skin equivalent undergo allergen-induced maturation and migration. J Leukoc Biol 2011-11-01 [PMID: 21697260]
• Eisenwort G et al. Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells. J Invest Dermatol 2011-10-01 [PMID: 21677668]
• Bigley V et al. The human syndrome of dendritic cell, monocyte, B and NK lymphoid deficiency. J Exp Med 2011-02-14 [PMID: 21242295]
• Stary G et al. Glucocorticosteroids modify Langerhans cells to produce TGF-beta and expand regulatory T cells. J Immunol 2011-01-01 [PMID: 21135170]
• Peressin M et al. HIV-1 replication in Langerhans and interstitial dendritic cells is inhibited by neutralizing and Fc-mediated inhibitory antibodies. J Virol 2011-01-01 [PMID: 21084491]
• van de Laar L et al. A nonredundant role for canonical NF-kB in human myeloid dendritic cell development and function. J Immunol 2010-12-15 [PMID: 21076069]
• Bobr A et al. Acute ablation of Langerhans cells enhances skin immune responses. J Immunol 2010-10-15 [PMID: 20855870]
• deJong MA et al. Herpes simplex virus type 2 enhances HIV-1 susceptibility by affecting Langerhans cell function. J Immunol 2010-08-01 [PMID: 20592277]
• Huch JH et al. Impact of varicella-zoster virus on dendritic cell subsets in human skin during natural infection. J Virol 2010-04-01 [PMID: 20130046]
• Schuster C et al. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells. J Exp Med 2009-01-01 [PMID: 19139172]
• Mayer WJ et al. Characterization of antigen-presenting cells in fresh and cultured human corneas using novel dendritic cell markers. Invest Ophthalmol Vis Sci 2007-10-01 [PMID: 17898266]
• Movassagh M et al. Selective accumulation of mature DC-Lamp+ dendritic cells in tumor sites is associated with efficient T-cell-mediated antitumor response and control of metastatic dissemination in melanoma. Cancer Res 2004-03-15 [PMID: 15026362]
• Valladeau J et al. Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules. Immunity 2000-01-01 [PMID: 10661407]
• Valladeau J et al. The monoclonal antibody DCGM4 recognizes Langerin, a protein specific of Langerhans cells, and is rapidly internalized from the cell surface. Eur J Immunol 1999-09-01 [PMID: 10508244]
• Ito T et al. A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells. J Immunol 1999-08-01 [PMID: 10415041]

Bioinformatics

• Gene Symbol: CD207