Langerin/CD207 Antibody (929F3.01)

Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Langerin Antibody (929F3.01) [DDX0362P-100] - IF staining on isolated skin-isolated porcine Langerhans cells .

Immunohistochemistry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Epidermal LC are dispensable for betaGal-specific immunity but down-regulate OVA-specific T cell functions. LangDTR or B6 WT mice were injected once with 1ug DT. Epidermal sheets were stained for langerin expression 3 wks later. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0128722), licensed under a CC-BY license.

Flow Cytometry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Cells derived from culture of porcine skin stained permeabilized (D) or not (C) with 929F3

Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Mouse epidermal sheet.

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Analysis using the HRP conjugate of DDX0362P-100. Staining of human skin frozen section.

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - IHC staining of human skin cryosections with 929F3-HRP

• Reactivity: Hu, Mu, Rt, Po, Eq
• Applications: ELISA, Flow, ICC/IF, IHC
• Clone: 929F3.01
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Langerin/CD207 Antibody (929F3.01) Summary

• Immunogen: Langerin/CD207 was used as immunogen.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD207
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: Permeabilization is recommended for flow cytometry.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Dendritics and distributed by Novus Biologicals in North America and other select countries. To order in the following countries: Germany, Finland, United Kingdom, Norway, Denmark, Switzerland, Austria, Japan, and South Korea, please contact Dendritics for additional ordering information.

Alternate Names for Langerin/CD207 Antibody (929F3.01)

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• Langerin

Background

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Langerin/CD207 Antibody (DDX0362P-100)(73)

Publications using DDX0362P-100

• Radtke AJ, Chu CJ, Yaniv Z Et al. IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues Nat Protoc 2022-01-13 [PMID: 35022622] (Hydrolysis Assay, Human)
  
  Details:
  Citation using the Alexa Fluor 532 version of this antibody.
• Guilliams M, Bonnardel J, Haest B Et al. Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches Cell 2022-01-12 [PMID: 35021063]
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Hasegawa T, Feng Z, Yan Z et al. Reduction in human epidermal Langerhans cells with age is associated with decline in CXCL14-mediated recruitment of CD14+ monocytes J. Invest. Dermatol. 2019-12-24 [PMID: 31881212]
• Schulz A, Jiang L, de Vor L et al. Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization Cell Rep 2019-10-29 [PMID: 31665625] (IHC-Fr, Human)
• Bonnardel J, T'Jonck W, Gaublomme D et al.. Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche Immunity. 2019-10-15 [PMID: 31561945] (FLOW, Mouse)
  
  Details:
  Citation used the Alexa Fluor 647 format of this antibody.
• Nelson DS, Marano RL, Joo Y et al. BRAF V600E and Pten deletion in mice produces a histiocytic disorder with features of Langerhans cell histiocytosis PLoS One. 2019-09-17 [PMID: 31527903] (ICC/IF, FLOW, Mouse)
  
  Details:
  Citation used the Alexa Fluor 647 format of this antibody.
• Yang Y, Zenke Y, Hirai T, Kaplan, DH Keratinocyte-derived TGF beta is not required to maintain skin immune homeostasis J. Dermatol. Sci. 2019-05-07 [PMID: 31118160] (FLOW, Mouse)
• Artym J, Kocieba M, Zaczynska E et al. Topically applied azaphenothiazines inhibit experimental psoriasis in mice. Int. Immunopharmacol. 2018-04-16 [PMID: 29674255] (Mouse)
• Capucha T, Koren N, Nassar M et al. Sequential BMP7/TGF-b1 signaling and microbiota instruct mucosal Langerhans cell differentiation J. Exp. Med. 2018-01-17 [PMID: 29343501] (Mouse)
• Artym J, Kocieba M, Zaczynska E et al. Topically applied azaphenothiazines inhibit contact sensitivity to oxazolone in mice Histol. Histopathol. 2017-07-27 [PMID: 28748525] (Mouse)
• Baptista MA, Keszei M, Oliveira M et al. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells. Nat Commun. 2016-07-18 [PMID: 27425374] (IF/IHC)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Gibbs A et al. MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation. Mucosal Immunol 2017-01-01 [PMID: 27049062]
• Chandra J et al. Epithelium Expressing the E7 Oncoprotein of HPV16 Attracts Immune-Modulatory Dendritic Cells to the Skin and Suppresses Their Antigen-Processing Capacity. PLoS One 2016-03-01 [PMID: 27031095]
• Quispel WT, Stegehuis-Kamp JA, Santos SJ et al. Activated Conventional T-Cells Are Present in Langerhans Cell Histiocytosis Lesions Despite the Presence of Immune Suppressive Cytokines. J. Interferon Cytokine Res. 2015-10-01 [PMID: 26381039]
• Haid B et al. Langerhans cells in the sebaceous gland of the murine skin. Exp Dermatol 2015-11-01 [PMID: 26174007]
• Weber CS et al. Immune Reactions against Gene Gun Vaccines Are Differentially Modulated by Distinct Dendritic Cell Subsets in the Skin. PLoS One 2015-06-01 [PMID: 26030383]
• Ab dWarif NM et al. Langerhans cell homeostasis and activation is altered in hyperplastic human papillomavirus type 16 E7 expressing epidermis. PLoS One 2015-05-01 [PMID: 25992642]
• Naik S et al. Commensal-dendritic-cell interaction specifies a unique protective skin immune signature. Nature 2015-04-02 [PMID: 25539086]
• Flacher V et al. Murine Langerin+ dermal dendritic cells prime CD8+ T cells while Langerhans cells induce cross-tolerance. EMBO Mol Med 2014-08-01 [PMID: 25085878]
• Sparber F et al. The late endosomal adaptor molecule p14 (LAMTOR2) regulates TGFbeta1-mediated homeostasis of Langerhans cells. J Invest Dermatol 2015-01-01 [PMID: 25078666]
• Hobbay P et al. Fcgamma receptor III and Fcgamma receptor IV on macrophages drive autoimmune valvular carditis in mice. Arthritis Rheumatol 2014-04-01 [PMID: 24757138]
• Voisin B et al. Anatomical distribution analysis reveals lack of Langerin+ dermal dendritic cells in footpads and tail of C57BL/6 mice. Exp Dermatol 2014-05-01 [PMID: 24629018]
• Knickelbein JE et al. Antigen-presenting cells are stratified within normal human corneas and are rapidly mobilized during ex vivo viral infection. Invest Ophthalmol Vis Sci 2014-02-24 [PMID: 24508792]
• Sperling T et al. Human papillomavirus type 8 interferes with a novel C/EBPbeta-mediated mechanism of keratinocyte CCL20 chemokine expression and Langerhans cell migration. PLoS Pathog 2012-01-01 [PMID: 22911498]
• Radtke Aj, Chu Cj, Yaniv Z Et Al. IBEX: An open and extensible method for high content multiplex imaging of diverse tissues arXiv preprint 2021-01-01
• Muto J et al. Hyaluronan digestion controls DC migration from the skin. J Clin Invest 2014-03-01 [PMID: 24487587]
• Elnekave M et al. Second-Generation Langerhans Cells Originating from Epidermal Precursors Are Essential for CD8+ T Cell Priming. J. Immunol. 2014-02-15 [PMID: 24420922]
• Mollah S et al. Flt3L dependence helps define an uncharacterized subset of murine cutaneous dendritic cells. J Invest Dermatol 2014-05-01 [PMID: 24288007]
• Debeer S et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol 2013-07-01 [PMID: 24047577]
• Mizraji G, Segev H, Wilensky A et al. J Vis Exp 2013 Jul 02 [PMID: 23851361]. J Vis Exp. 2013-07-02 [PMID: 23851361]
  
  Details:
  This citation used the PE version of this antibody.
• Bachy V et al. Langerin negative dendritic cells promote potent CD8+ T-cell priming by skin delivery of live adenovirus vaccine microneedle arrays. Proc Natl Acad Sci U S A 2013-02-19 [PMID: 23386724]
• Kellersch B et al. Langerhans cell homeostasis in mice is dependent on mTORC1 but not mTORC2 function. Blood 2013-01-10 [PMID: 23212520]
• Luckashenak N et al. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol 2013-01-01 [PMID: 23209325]
• Xu YP et al. Aging affects epidermal Langerhans cell development and function and alters their miRNA gene expression profile. Aging (Albany NY) 2012-11-01 [PMID: 23178507]
• Mi QS et al. Deletion of microRNA miR-223 increases Langerhans cell cross-presentation. Int J Biochem Cell Biol 2013-02-01 [PMID: 23153510]
• Hitzler M et al. Human Langerhans cells control Th cells via programmed death-ligand 1 in response to bacterial stimuli and nickel-induced contact allergy. PLoS One 2012-01-01 [PMID: 23056446]
• Iram N et al. Age-related changes in expression and function of Toll-like receptors in human skin. Development 2012-11-01 [PMID: 23034637]
• Arizon M et al. Langerhans cells down-regulate inflammation-driven alveolar bone loss. Proc Natl Acad Sci U S A 2012-05-01 [PMID: 22509018]
• Haley K et al. Langerhans cells require MyD88-dependent signals for Candida albicans response but not for contact hypersensitivity or migration. J Immunol 2012-05-01 [PMID: 22442445]
• Segura E et al. Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med 2012-04-09 [PMID: 22430490]
• Flacher V et al. Skin langerin+ dendritic cells transport intradermally injected anti-DEC-205 antibodies but are not essential for subsequent cytotoxic CD8+ T cell responses. J Immunol 2012-03-01 [PMID: 22291181]
• Langlet C et al. CD64 expression distinguishes monocyte-derived and conventional dendritic cells and reveals their distinct role during intramuscular immunization. J Immunol 2012-02-15 [PMID: 22262658]
• Edelson B et al. Batf3-dependent CD11b(low/-) peripheral dendritic cells are GM-CSF-independent and are not required for Th cell priming after subcutaneous immunization. PLoS One 2011-01-01 [PMID: 22065991]
• Luche H et al. The earliest intrathymic precursors of CD8alpha(+) thymic dendritic cells correspond to myeloid-type double-negative 1c cells. Eur J Immunol 2011-08-01 [PMID: 21630253]
• Mikami N et al. Calcitonin gene-related peptide is an important regulator of cutaneous immunity: effect on dendritic cell and T cell functions. J Immunol 2011-06-15 [PMID: 21551361]
• Kastenmuller K et al. Protective T cell immunity in mice following protein-TLR7/8 agonist-conjugate immunization requires aggregation, type I IFN, and multiple DC subsets. J Clin Invest 2011-05-01 [PMID: 21540549]
• Rochereau N et al. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine 2011-05-09 [PMID: 21439318]
• Marquet F et al. Characterization of dendritic cells subpopulations in skin and afferent lymph in the swine model. PLoS One 2011-01-27 [PMID: 21298011]
• Stoecklinger A et al. Langerin+ dermal dendritic cells are critical for CD8+ T cell activation and IgH gamma-1 class switching in response to gene gun vaccines. J Immunol 2011-02-01 [PMID: 21187444]
• Bobr A et al. Acute ablation of Langerhans cells enhances skin immune responses. J Immunol 2010-10-15 [PMID: 20855870]
• Noordegraaf M et al. Functional redundancy of Langerhans cells and Langerin+ dermal dendritic cells in contact hypersensitivity. J Invest Dermatol 2010-12-01 [PMID: 20703247]
• deJong MA et al. Herpes simplex virus type 2 enhances HIV-1 susceptibility by affecting Langerhans cell function. J Immunol 2010-08-01 [PMID: 20592277]
• Poulin LF et al. Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells. J Exp Med 2010-06-07 [PMID: 20479117]
• Henri S et al. CD207+ CD103+ dermal dendritic cells cross-present keratinocyte-derived antigens irrespective of the presence of Langerhans cells. J Exp Med 2010-01-18 [PMID: 20038600]
• Chorro L et al. Langerhans cell (LC) proliferation mediates neonatal development, homeostasis, and inflammation-associated expansion of the epidermal LC network. J Exp Med 2009-12-21 [PMID: 19995948]
• Tripp CH et al. The lymph vessel network in mouse skin visualised with antibodies against the hyaluronan receptor LYVE-1. Immunobiology 2008-01-01 [PMID: 18926287]
• Nfon CK et al. Langerhans cells in porcine skin. Vet Immunol Immunopathol 2008-12-15 [PMID: 18805593]
• Shklovskaya E et al. Epidermal and dermal dendritic cells display differential activation and migratory behavior while sharing the ability to stimulate CD4+ T cell proliferation in vivo. J Immunol 2008-07-01 [PMID: 18566408]
• Stoitzner P et al. Inefficient presentation of tumor-derived antigen by tumor-infiltrating dendritic cells. Cancer Immunol Immunother 2008-11-01 [PMID: 18311487]
• Flacher V et al. Expression of langerin/CD207 reveals dendritic cell heterogeneity between inbred mouse strains. Immunology 2008-03-01 [PMID: 18217955]
• Bursch LS et al. Identification of a novel population of Langerin+ dendritic cells. J Exp Med 2007-12-24 [PMID: 18086865]
• Poulin LF et al. The dermis contains langerin+ dendritic cells that develop and function independently of epidermal Langerhans cells. J Exp Med 2007-12-24 [PMID: 18086861]
• Kaplan DH et al. Autocrine/paracrine TGFbeta1 is required for the development of epidermal Langerhans cells. J Exp Med 2007-10-29 [PMID: 17938236]
• Stoecklinger A et al. Epidermal langerhans cells are dispensable for humoral and cell-mediated immunity elicited by gene gun immunization. J Immunol 2007-07-15 [PMID: 17617579]
• Zimmerli SC et al. Langerhans cells and lymph node dendritic cells express the tight junction component claudin-1. J Invest Dermatol 2007-10-01 [PMID: 17508021]
• Dubrac S et al. Peroxisome proliferator-activated receptor-alpha activation inhibits Langerhans cell function. J Immunol 2007-04-01 [PMID: 17371993]
• Kaplan DH et al. Epidermal langerhans cell-deficient mice develop enhanced contact hypersensitivity. Immunity 2005-12-01 [PMID: 16356859]
• Douillard P et al. Mouse lymphoid tissue contains distinct subsets of langerin/CD207 dendritic cells, only one of which represents epidermal-derived Langerhans cells. J Invest Dermatol 2005-11-01 [PMID: 16297200]
• Nakagawa Y, Egawa G, Miyake T et al. A phenotypic analysis of involucrin-mOVA mice following adoptive transfer of OVA-specific CD8+ T cells J Clin Invest 2005-06-14 [PMID: 15951839]
• Abadie V et al. Neutrophils rapidly migrate via lymphatics after Mycobacterium bovis BCG intradermal vaccination and shuttle live bacilli to the draining lymph nodes. Blood 2005-09-01 [PMID: 15886329]
• Kissenpfennig A et al. Disruption of the langerin/CD207 gene abolishes Birbeck granules without a marked loss of Langerhans cell function. Mol Cell Biol 2005-01-01 [PMID: 15601833]
• Movassagh M et al. Selective accumulation of mature DC-Lamp+ dendritic cells in tumor sites is associated with efficient T-cell-mediated antitumor response and control of metastatic dissemination in melanoma. Cancer Res 2004-03-15 [PMID: 15026362]
• Valladeau J et al. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol 2002-01-15 [PMID: 11777972]

Bioinformatics

• Gene Symbol: CD207