Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647]

Product Image: Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647] [DDX0362A647-100] - Vial of Alexa Fluor 647 conjugated antibody. Alexa Fluor 647 is optimally excited at 653 nm by the Red laser (633 or 640 nm) and has an emission maximum of 669 nm.

• Reactivity: Hu, Mu, Rt, Po, Eq
• Applications: Flow, ICC/IF, IHC, NULL
• Clone: 929F3.01
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647] Summary

• Immunogen: Langerin/CD207 was used as immunogen.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD207
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Product Image
• Application Notes: Permeabilization is recommended for flow cytometry.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: No Preservative
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Langerin/CD207 Antibody (929F3.01) [Alexa Fluor® 647]

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• DDX0362A647
• Langerin

Background

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Langerin/CD207 Antibody (DDX0362A647-100)(3)

Publications using DDX0362A647-100

• Guilliams M, Bonnardel J, Haest B Et al. Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches Cell 2022-01-12 [PMID: 35021063]
  
  Details:
  Citation using the Alexa Fluor 647 version of this antibody.
• Bonnardel J, T'Jonck W, Gaublomme D et al.. Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche Immunity. 2019-10-15 [PMID: 31561945] (FLOW, Mouse)
  
  Details:
  Citation used the Alexa Fluor 647 format of this antibody.
• Nelson DS, Marano RL, Joo Y et al. BRAF V600E and Pten deletion in mice produces a histiocytic disorder with features of Langerhans cell histiocytosis PLoS One. 2019-09-17 [PMID: 31527903] (FLOW, ICC/IF, Mouse)
  
  Details:
  Citation used the Alexa Fluor 647 format of this antibody.

Review for Langerin/CD207 Antibody (DDX0362A647-100) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 08/13/2024

Summary

• Application: Flow Cytometry
• Sample Tested: Monocyte-derived dendritic cells
• Species: Human
• Lot: DDX0362-137

Bioinformatics

• Gene Symbol: CD207