Langerin/CD207 Antibody (808E10.01) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (808E10.01) [DDX0360P-100] - Analysis of Alexa Fluor (R) 488 conjugate of DDX0360P-100. Staining of Langerin-transfected CHO cells.

Immunohistochemistry: Langerin/CD207 Antibody (808E10.01) [DDX0360P-100] - Human skin frozen section:Langerhans cells staining with 808E10.01-HRP

Flow Cytometry: Langerin/CD207 Antibody (808E10.01) [DDX0360P-100] - Non -blocking anti-langerin clone

Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (808E10.01) [DDX0360P-100] - Murine peyer patch - Langerin

Flow Cytometry: Langerin/CD207 Antibody (808E10.01) [DDX0360P-100] - Blocking HIV-gp120(808E10.01 anti-langerin clone)

• Reactivity: Hu, Mu, Po, Ca
• Applications: Flow, Func, ICC/IF, IHC
• Clone: 808E10.01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Langerin/CD207 Antibody (808E10.01) - Azide and BSA Free Summary

• Immunogen: Langerin/CD207 was used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD207
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Langerin/CD207 Antibody (808E10.01) - Azide and BSA Free

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• Langerin

Background

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Langerin/CD207 Antibody (DDX0360P-100)(14)

Publications using DDX0360P-100

• Chabrol E et al. Alteration of the langerin oligomerization state affects Birbeck granule formation. Biophys J 2015-02-01 [PMID: 25650933]
• De Jesus M et al. A population of Langerin-positive dendritic cells in murine Peyer's patches involved in sampling beta-glucan microparticles. PLoS One 2014-03-01 [PMID: 24632738]
• Debeer S et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol 2013-07-01 [PMID: 24047577]
• Luckashenak N et al. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol 2013-01-01 [PMID: 23209325]
• Hitzler M et al. Human Langerhans cells control Th cells via programmed death-ligand 1 in response to bacterial stimuli and nickel-induced contact allergy. PLoS One 2012-01-01 [PMID: 23056446]
• Iram N et al. Age-related changes in expression and function of Toll-like receptors in human skin. Development 2012-11-01 [PMID: 23034637]
• Schwingshackl P et al. Distribution and maturation of skin dendritic cell subsets in two forms of cutaneous T-cell lymphoma: mycosis fungoides and Sezary syndrome. Acta Derm Venereol 2012-05-01 [PMID: 22678564]
• Segura E et al. Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med 2012-04-09 [PMID: 22430490]
• Rochereau N et al. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine 2011-05-09 [PMID: 21439318]
• Bobr A et al. Acute ablation of Langerhans cells enhances skin immune responses. J Immunol 2010-10-15 [PMID: 20855870]
• deJong MA et al. Herpes simplex virus type 2 enhances HIV-1 susceptibility by affecting Langerhans cell function. J Immunol 2010-08-01 [PMID: 20592277]
• Nfon CK et al. Langerhans cells in porcine skin. Vet Immunol Immunopathol 2008-12-15 [PMID: 18805593]
• Douillard P et al. Mouse lymphoid tissue contains distinct subsets of langerin/CD207 dendritic cells, only one of which represents epidermal-derived Langerhans cells. J Invest Dermatol 2005-11-01 [PMID: 16297200]
• Valladeau J et al. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol 2002-01-15 [PMID: 11777972]

Bioinformatics

• Gene Symbol: CD207