Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Cryosections of human skin, 1:200 dilution, staining for 1 hour at 37 degrees. Dapi nuclear stain used to define architecture. Langerin staining is visible in the epidermis. Image submitted from verified customer review.

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Staining of human skin paraffin section with 310F7.02

Flow Cytometry: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Saining with 310F7.02 o f cells derived from overnight culture o f porcine skin biopsies permeabilized (B) or not(A).

Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Staining of CHO cells transfected (right) or not (left) with mouse Langerin.

Immunohistochemistry-Paraffin: Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Porcine skin cryosections stained with Langerin antibody

Flow (Cell Surface): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Analysis using the Allophycocyanin conjugate of DDX0361P-100. Staining of Langerin in stably transfected CHO using Langerin antibody at 1ug/ml. Blue histogram represents isotype control (1ug/ml), red histogram is anti-Langerin. Total viable cells were use

Flow (Intracellular): Langerin/CD207 Antibody (310F7.02) [DDX0361P-100] - Analysis using the PE conjugate of DDX0361P-100. Staining of Langerin in stably transfected CHO cells (A) or DC-SIGN in stably transfected HeLa cells (negative control) (B) using antibody at 1ug/ml. Blue histogram in both panels represents isotype control.

• Reactivity: Hu, Mu, Rt, Po, ChHa
• Applications: ELISA, Flow, ICC/IF, IHC
• Clone: 310F7.02
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free Summary

• Immunogen: Langerin/CD207 was used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD207
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow (Cell Surface)
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Langerin/CD207 Antibody (310F7.02) - Azide and BSA Free

• CD207 antigen
• CD207 antigen, langerin
• CD207 molecule, langerin
• CD207
• CLEC4KLangerhans cell specific c-type lectin
• C-type lectin domain family 4 member K
• C-type lectin domain family 4, member K
• Langerin

Background

Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Langerin/CD207 Antibody (DDX0361P-100)(16)

Publications using DDX0361P-100

• Jackson R, Lukacs JD, Zehbe I. The Potentials and Pitfalls of a Human Cervical Organoid Model Including Langerhans Cells Viruses 2020-12-01 [PMID: 33271909]
• Pestronk A, Sinha N et al. Immune myopathy with large histiocyte-related myofiber necrosis. Neurology 2019-09-04 [PMID: 30894448] (IF/IHC, Human)
• Chabrol E et al. Alteration of the langerin oligomerization state affects Birbeck granule formation. Biophys J 2015-02-01 [PMID: 25650933]
• Gerber A et al. High expression of FOXP3 in primary melanoma is associated with tumour progression. Br J Dermatol 2014-01-01 [PMID: 24116716]
• Debeer S et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol 2013-07-01 [PMID: 24047577]
• Boyd A et al. Quiescent innate response to infective filariae by human Langerhans cells suggests a strategy of immune evasion. Infect Immun 2013-05-01 [PMID: 23429540]
• Luckashenak N et al. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol 2013-01-01 [PMID: 23209325]
• Hitzler M et al. Human Langerhans cells control Th cells via programmed death-ligand 1 in response to bacterial stimuli and nickel-induced contact allergy. PLoS One 2012-01-01 [PMID: 23056446]
• Iram N et al. Age-related changes in expression and function of Toll-like receptors in human skin. Development 2012-11-01 [PMID: 23034637]
• Segura E et al. Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med 2012-04-09 [PMID: 22430490]
• Bobr A et al. Acute ablation of Langerhans cells enhances skin immune responses. J Immunol 2010-10-15 [PMID: 20855870]
• deJong MA et al. Herpes simplex virus type 2 enhances HIV-1 susceptibility by affecting Langerhans cell function. J Immunol 2010-08-01 [PMID: 20592277]
• Nfon CK et al. Langerhans cells in porcine skin. Vet Immunol Immunopathol 2008-12-15 [PMID: 18805593]
• Douillard P et al. Mouse lymphoid tissue contains distinct subsets of langerin/CD207 dendritic cells, only one of which represents epidermal-derived Langerhans cells. J Invest Dermatol 2005-11-01 [PMID: 16297200]
• Treilleux I et al. Dendritic cell infiltration and prognosis of early stage breast cancer. Clin Cancer Res 2004-11-01 [PMID: 15569976]
• Valladeau J et al. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol 2002-01-15 [PMID: 11777972]

Review for Langerin/CD207 Antibody (DDX0361P-100) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 04/03/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: human skin cryosections
• Species: Human
• Lot: DDX0361-020

Comments

• Comments: This antibody worked great for IF on cryosections of human skin at a 1:200 dilution, staining for 1 hour at 37 degrees. Dapi nuclear stain was used to define architecture. Langerin staining is visible in the epidermis.

Bioinformatics

• Gene Symbol: CD207