Laminin gamma 1 Antibody (CL3196) - BSA Free

Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Analysis of purified human recombinant Laminin-111, Laminin-121, Laminin-211, protein Laminin-221, Laminin-411, Laminin-421, Laminin-511, Laminin-521 and Laminin-332.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Staining of human tonsil shows absence of immunoreactivity in lymphoid tissue (negative control).

Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Analysis in human lung tissue.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Staining of human placenta shows strong immunoreactivity in basement membrane of trophoblast and in endothelium.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Staining of human heart shows strong membranous immunoreactivity in cardiomyocytes.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Staining of human stomach shows strong immunoreactivity in basement membrane of glandular epithelium.

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Staining of human endometrium shows strong positivity in basement membrane of glandular epithelium.

• Reactivity: Hu, Rt, Mu
• Applications: WB, ICC/IF, IHC
• Clone: CL3196
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Laminin gamma 1 Antibody (CL3196) - BSA Free Summary

• Description: Novus Biologicals Mouse Laminin gamma 1 Antibody (CL3196) - BSA Free (NBP2-42395) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Laminin gamma 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P11047, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (91%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID:30044554)
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 30044554). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Laminin gamma 1 Antibody (CL3196) - BSA Free

• LAMB2Laminin-7 subunit gamma
• LAMC1
• Laminin B2 chain
• Laminin B2
• Laminin gamma 1
• laminin subunit gamma-1
• laminin, gamma 1 (formerly LAMB2)
• Laminin-1 subunit gamma
• Laminin-10 subunit gamma
• Laminin-11 subunit gamma
• Laminin-2 subunit gamma
• Laminin-3 subunit gamma
• Laminin-4 subunit gamma
• Laminin-6 subunit gamma
• Laminin-8 subunit gamma
• Laminin-9 subunit gamma
• MGC87297
• S-LAM gamma
• S-laminin subunit gamma

Background

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin gamma 1 Antibody (NBP2-42395)(1)

Publication using NBP2-42395

• Sun X, Wang Y, Guo Z et al. Acellular Cauda Equina Allograft as Main Material Combined with Biodegradable Chitin Conduit for Regeneration of Long-Distance Sciatic Nerve Defect in Rats. Adv Healthc Mater. 2018-07-25 [PMID: 30044554] (ICC/IF, Rat)

Bioinformatics

• Gene Symbol: LAMC1
• Uniprot:
  • P11047(Human)