Laminin alpha 5 Antibody

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Laminin alpha 5 Antibody Summary

• Immunogen: Synthetic peptide derived from Cter domain of Lama5 protein
• Specificity: Reacts specifically with mouse 404 kDa protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LAMA5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Western Blot 1:200 - 1:1000
• Application Notes: Use in IHC reported in scientific literature (PMID: 30139844).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in distilled water.

Alternate Names for Laminin alpha 5 Antibody

• KIAA0533
• KIAA1907
• laminin alpha-5 chain
• laminin subunit alpha-5
• laminin, alpha 5
• Laminin-10 subunit alpha
• Laminin-11 subunit alpha
• Laminin-15 subunit alpha

Background

Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins are thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin alpha 5 (LAMA5) belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Laminin alpha 5 is the alpha chain in the Laminin 15 complex which is a heterotrimer composed of alpha 5/beta 2/gamma 3 which are bound to each other by disulfide bonds into a cross shaped molecule comprising one long and three short arms with globules at each end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin alpha 5 Antibody (NBP1-18714)(3)

Publications using NBP1-18714

• Zhou X, Chen W, Gan C et al. Aberrant serum-derived FN1 variants bind to integrin beta 1 on glomerular endothelial cells contributing to thin basement membrane nephropathy. International journal of biological macromolecules 2024-10-04 [PMID: 39368581]
• Li L, Shirkey MW, Zhang T et al. The lymph node stromal laminin alpha 5 shapes alloimmunity J. Clin. Invest. 2020-02-04 [PMID: 32017712] (IF/IHC, Mouse)
• Segarra M, Aburto MR, Cop F et al. Endothelial Dab1 signaling orchestrates neuro-glia-vessel communication in the central nervous system Science 2018-08-24 [PMID: 30139844] (IF/IHC, Mouse)

Review for Laminin alpha 5 Antibody (NBP1-18714) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Wholemount Immunofluorescence Mouse 05/03/2021

Summary

• Application: Other
• Sample Tested: Mouse tissue,Mouse Tissues
• Species: Mouse
• Lot: 19C11

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

FAQs for Laminin alpha 5 Antibody (NBP1-18714). (Showing 1 - 1 of 1 FAQs).

1. Do you know some papers that are citing this antibody for used in IHC?
   • Unfortunately we are not currently aware of any publications which cite the use of our laminin alpha 5 antibody, NBP1-18714. This does not mean that such articles do not exist, it is just that we are unaware of them if they do.

Bioinformatics

• Gene Symbol: LAMA5
• Entrez:
  • 16776(Mouse)
• Uniprot:
  • Q61001(Mouse)