Laminin S/Laminin beta 2 Antibody (C4) [Unconjugated]

Laminin S was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Rat/Chicken Laminin S Monoclonal Antibody (Catalog # MAB2066) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Rt, Ch
• Applications: WB, IP, ICC/IF
• Clone: C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Laminin S/Laminin beta 2 Antibody (C4) [Unconjugated] Summary

• Immunogen: Bovine anterior lens capsule
• Specificity: Detects human, rat, and chicken Laminin S in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMB2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin S/Laminin beta 2 Antibody (C4) [Unconjugated]

• LAMB2
• Laminin B1s chain
• Laminin beta 2
• Laminin S
• laminin subunit beta-2
• laminin, beta 2 (laminin S)
• Laminin-11 subunit beta
• Laminin-14 subunit beta
• Laminin-15 subunit beta
• Laminin-3 subunit beta
• Laminin-4 subunit beta
• Laminin-7 subunit beta
• LAMSLaminin-9 subunit beta
• S-LAM beta
• S-laminin subunit beta

Background

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins critically contribute to cell differentation, cell shape and migration and maintenance of tissue phenotypes and survival (1). Laminin S, also known as laminin beta 2, is a subunit of laminin-3 (S-laminin), laminin-4 (S-merosin) and laminin-7 (KS-laminin). It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle.

1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin S/Laminin beta 2 Antibody (MAB2066)(3)

Publications using MAB2066

• M Namba, T Kobayashi, M Kohno, T Koyano, T Hirose, M Fukushima, M Matsuyama Creation of X-linked Alport syndrome rat model with Col4a5 deficiency Scientific Reports, 2021-10-21;11(1):20836. 2021-10-21 [PMID: 34675305] (Western Blot, Rat)
• Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury. Frontiers in Bioengineering and Biotechnology, 2018-11-01;0(0):2296-4185. 2018-11-01 [PMID: 30443545] (IHC-P, Human)
• Green TL, Hunter DD, Chan W, Merlie JP, Sanes JR Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells. J. Biol. Chem., 1992-01-25;267(3):2014-22. 1992-01-25 [PMID: 1730732] (ICC, Immunoprecipitation, Western Blot, Rat)

Bioinformatics

• Gene Symbol: LAMB2