Laminin alpha 4 Antibody [Unconjugated]

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Laminin alpha 4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Laminin alpha 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3837) at 3 µg/mL overnight ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Laminin alpha 4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Laminin alpha 4
Gln826-Ala1816
Accession # P97927
Specificity
Detects mouse Laminin  alpha 4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LAMA4
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
AF3837 in the following applications:

Publications
Read Publications using
AF3837 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 4 Antibody [Unconjugated]

  • DKFZp686D23145
  • LAMA3LAMA4*-1
  • LAMA4
  • laminin alpha 4 chain
  • Laminin alpha 4
  • laminin subunit alpha-4
  • laminin, alpha 4
  • Laminin-14 subunit alpha
  • Laminin-8 subunit alpha
  • Laminin-9 subunit alpha

Background

Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single alpha , beta , and gamma chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin  alpha 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin alpha 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C‑terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin  alpha 4 shows 91% and 97% aa sequence identity with human and rat Laminin  alpha 4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Laminin alpha 4 Antibody (AF3837)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(4)
IHC-Fr
(2)
IHC-P
(1)
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Filter By Species
Mouse
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF3837 Applications Species
SA Wohlfeil, V Häfele, B Dietsch, C Weller, C Sticht, AS Jauch, M Winkler, CD Schmid, AL Irkens, A Olsavszky, K Schledzews, PS Reiners-Ko, S Goerdt, C Géraud Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment Journal of Translational Medicine, 2022;20(1):62. 2022 [PMID: 35109875] (ICC/IF, Mouse) ICC/IF Mouse
M Nesbit, JC Mamo, M Majimbi, V Lam, R Takechi Automated Quantitative Analysis of ex vivo Blood-Brain Barrier Permeability Using Intellesis Machine-Learning Frontiers in Neuroscience, 2021;15(0):617221. 2021 [PMID: 33935625] (IHC, Mouse) IHC Mouse
B Fields, A DeLaForest, M Zogg, J May, C Hagen, K Komnick, J Wieser, A Lundberg, H Weiler, MA Battle, KS Carlson The Adult Murine Intestine is Dependent on Constitutive Laminin-gamma1 Synthesis Sci Rep, 2019;9(1):19303. 2019 [PMID: 31848396] (IHC-P, Mouse) IHC-P Mouse
Kim J, Hopkinson M, Kavishwar M, Fernandez-Fuente M, Brown S Prenatal muscle development in a mouse model for the secondary dystroglycanopathies. Skelet Muscle, 2015;6(0):3. 2015 [PMID: 26900448] (IHC, Mouse) IHC Mouse
Yao Y, Chen Z, Norris E, Strickland S Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Nat Commun, 2014;5(0):3413. 2014 [PMID: 24583950] (IHC, Mouse) IHC Mouse
Transforming growth factor β-1 stimulates profibrotic epithelial signaling to activate pericyte-myofibroblast transition in obstructive kidney fibrosis. Am J Pathol, 2013;182(1):118-31. 2013 [PMID: 23142380] (IHC, Mouse) IHC Mouse
Flanagan K, Fitzgerald K, Baker J Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS. PLoS ONE, 2012;7(7):e40443. 2012 [PMID: 22792325] (IHC-Fr, Mouse) IHC-Fr Mouse
Ackroyd MR, Whitmore C, Prior S, Kaluarachchi M, Nikolic M, Mayer U, Muntoni F, Brown SC Fukutin-related protein alters the deposition of laminin in the eye and brain. J. Neurosci., 2011;31(36):12927-35. 2011 [PMID: 21900571] (IHC-Fr, Mouse) IHC-Fr Mouse

Review for Laminin alpha 4 Antibody (AF3837) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3837:
Filter by Applications
Immunoflourescence in Frozen mouse brain sections
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Other Laminin alpha 4 AF3837
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3
reviewed by:
Verified Customer
Immunoflourescence in Frozen mouse brain sections Mouse 11/25/2021
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Summary

ApplicationOther
Sample TestedP12 20µm mouse brain frozen sections
SpeciesMouse
LotYLCO120022

Comments

CommentsA lot of standardisation may be required for the antibody to work.
good fixation of the tissue may help. for adult tissues, they need to be perfused well in order to achieve good results.

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Verified Customer
11/25/2021
Application: Immunoflourescence in Frozen mouse brain sections
Species: Mouse

Bioinformatics

Gene Symbol LAMA4
Uniprot