Laminin alpha 4 Antibody [Unconjugated]

Laminin a4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Laminin a4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3837) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to membranes in blood vessels in CNS. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Laminin-alpha 4 staining provides a clear indication of the BBB (A), however pixel intensity varies considerably. Intellesis is able to recognise high and low intensity pixels accurately (B), unlike classical intensity thresholds which result in either too much (C) or too little (D) capillary segmentation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33935625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Laminin-alpha 4 staining provides a clear indication of the BBB (A), however pixel intensity varies considerably. Intellesis is able to recognise high and low intensity pixels accurately (B), unlike classical intensity thresholds which result in either too much (C) or too little (D) capillary segmentation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33935625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Laminin-alpha 4 staining provides a clear indication of the BBB (A), however pixel intensity varies considerably. Intellesis is able to recognise high and low intensity pixels accurately (B), unlike classical intensity thresholds which result in either too much (C) or too little (D) capillary segmentation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33935625), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Laminin alpha 4 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Laminin alpha 4
  Gln826-Ala1816
  Accession # P97927
• Specificity: Detects mouse Laminin alpha 4 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LAMA4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Laminin alpha 4 Antibody [Unconjugated]

• DKFZp686D23145
• LAMA3LAMA4*-1
• LAMA4
• laminin alpha 4 chain
• Laminin alpha 4
• laminin subunit alpha-4
• laminin, alpha 4
• Laminin-14 subunit alpha
• Laminin-8 subunit alpha
• Laminin-9 subunit alpha

Background

Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single alpha , beta , and gamma chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin  alpha 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin alpha 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C‑terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin  alpha 4 shows 91% and 97% aa sequence identity with human and rat Laminin  alpha 4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin alpha 4 Antibody (AF3837)(16)

Publications using AF3837

• Wohlfeil, SA;Olsavszky, A;Irkens, AL;Häfele, V;Dietsch, B;Straub, N;Goerdt, S;Géraud, C; Deficiency of Stabilin-1 in the Context of Hepatic Melanoma Metastasis Cancers 2024-01-19 [PMID: 38275881] (IHC, Transgenic Mouse, Mouse)
• Arbaizar-Rovirosa, M;Gallizioli, M;Lozano, JJ;Sidorova, J;Pedragosa, J;Figuerola, S;Chaparro-Cabanillas, N;Boya, P;Graupera, M;Claret, M;Urra, X;Planas, AM; Transcriptomics and translatomics identify a robust inflammatory gene signature in brain endothelial cells after ischemic stroke Journal of neuroinflammation 2023-09-11 [PMID: 37691115] (IHC, Mouse, Transgenic Mouse)
• Jiekun Yang, Maria Vamvini, Pasquale Nigro, Li-Lun Ho, Kyriakitsa Galani, Marcus Alvarez et al. Single-cell dissection of the obesity-exercise axis in adipose-muscle tissues implies a critical role for mesenchymal stem cells Cell Metabolism 2022-10-04 [PMID: 36198295]
• Lidia Yshii, Emanuela Pasciuto, Pascal Bielefeld, Loriana Mascali, Pierre Lemaitre, Marika Marino et al. Astrocyte-targeted gene delivery of interleukin 2 specifically increases brain-resident regulatory T cell numbers and protects against pathological neuroinflammation Nature Immunology 2022-06-01 [PMID: 35618831]
• Swathi Ayloo, Christopher Gallego Lazo, Shenghuan Sun, Wei Zhang, Bianxiao Cui, Chenghua Gu Pericyte-to-endothelial cell signaling via vitronectin-integrin regulates blood-CNS barrier Neuron 2022-05-01 [PMID: 35294899]
• SA Wohlfeil, V Häfele, B Dietsch, C Weller, C Sticht, AS Jauch, M Winkler, CD Schmid, AL Irkens, A Olsavszky, K Schledzews, PS Reiners-Ko, S Goerdt, C Géraud Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment Journal of Translational Medicine, 2022-02-02;20(1):62. 2022-02-02 [PMID: 35109875] (ICC/IF, Mouse)
• M Nesbit, JC Mamo, M Majimbi, V Lam, R Takechi Automated Quantitative Analysis of ex vivo Blood-Brain Barrier Permeability Using Intellesis Machine-Learning Frontiers in Neuroscience, 2021-04-16;15(0):617221. 2021-04-16 [PMID: 33935625] (IHC, Mouse, Rat)
• Packer D, Martin PT. Micro-laminin gene therapy can function as an inhibitor of muscle disease in the dy(W) mouse model of MDC1A Mol Ther Methods Clin Dev 4/19/2021 [PMID: 33869655]
  
  Details:
  Citation using the FITC version of this antibody.
• Emanuela Pasciuto, Oliver T. Burton, Carlos P. Roca, Vasiliki Lagou, Wenson D. Rajan, Tom Theys et al. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition Cell 2020-08-06 [PMID: 32702313] (Immunohistochemistry, Mouse)
• B Fields, A DeLaForest, M Zogg, J May, C Hagen, K Komnick, J Wieser, A Lundberg, H Weiler, MA Battle, KS Carlson The Adult Murine Intestine is Dependent on Constitutive Laminin-gamma1 Synthesis Sci Rep, 2019-12-17;9(1):19303. 2019-12-17 [PMID: 31848396] (IHC-P, Mouse)
• Kim J, Hopkinson M, Kavishwar M, Fernandez-Fuente M, Brown S Prenatal muscle development in a mouse model for the secondary dystroglycanopathies. Skelet Muscle, 2016-02-19;6(0):3. 2016-02-19 [PMID: 26900448] (IHC, Mouse)
• Francesc Miró-Mur, Isabel Pérez-de-Puig, Maura Ferrer-Ferrer, Xabier Urra, Carles Justicia, Angel Chamorro et al. Immature monocytes recruited to the ischemic mouse brain differentiate into macrophages with features of alternative activation Brain, Behavior, and Immunity 2016-03-01 [PMID: 26275369] (Immunohistochemistry, Transgenic Mouse)
• Yao Y, Chen Z, Norris E, Strickland S Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. Nat Commun, 2014-03-03;5(0):3413. 2014-03-03 [PMID: 24583950] (IHC, Mouse)
• Transforming growth factor β-1 stimulates profibrotic epithelial signaling to activate pericyte-myofibroblast transition in obstructive kidney fibrosis. Am J Pathol, 2012-11-09;182(1):118-31. 2012-11-09 [PMID: 23142380] (IHC, Mouse)
• Flanagan K, Fitzgerald K, Baker J Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS. PLoS ONE, 2012-07-06;7(7):e40443. 2012-07-06 [PMID: 22792325] (IHC-Fr, Mouse)
• Ackroyd MR, Whitmore C, Prior S, Kaluarachchi M, Nikolic M, Mayer U, Muntoni F, Brown SC Fukutin-related protein alters the deposition of laminin in the eye and brain. J. Neurosci., 2011-09-07;31(36):12927-35. 2011-09-07 [PMID: 21900571] (IHC-Fr, Mouse)

Review for Laminin alpha 4 Antibody (AF3837) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Bhavin Shah Immunoflourescence in Frozen mouse brain sections Mouse 11/25/2021

Summary

• Application: Other
• Sample Tested: P12 20µm mouse brain frozen sections
• Species: Mouse
• Lot: YLCO120022

Comments

• Comments: A lot of standardisation may be required for the antibody to work.
  good fixation of the tissue may help. for adult tissues, they need to be perfused well in order to achieve good results.

Bioinformatics

• Gene Symbol: LAMA4
• Uniprot:
  • P97927()