Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free

Analysis in human placenta and lymph node tissues using NBP3-44417 antibody. Corresponding Laminin alpha 5 RNA-seq data are presented for the same tissues.

Staining of human colon shows moderate positivity in basement membrane of glandular cells.

Staining of human kidney shows strong membranous positivity in glomeruli, as well as in basement membrane of renal tubules.

Staining of human lymph node shows no positivity in lymphoid cells as expected.

Staining of human placenta shows moderate positivity in basement membrane of trophoblastic cells.

Analysis of purified human recombinant Laminin-511, Laminin-521, Laminin-121, Laminin-221, Laminin-332 and Laminin-411.

Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Laminin alpha 5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu, Rt
• Applications: WB, IHC
• Clone: CL3118
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of O15230, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMA5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32157808).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free

• KIAA0533
• KIAA1907
• laminin alpha-5 chain
• laminin subunit alpha-5
• laminin, alpha 5
• Laminin-10 subunit alpha
• Laminin-11 subunit alpha
• Laminin-15 subunit alpha

Background

Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMA5