Lamin B Receptor Antibody (PSH02-93)

Western Blot: Lamin B Receptor Antibody (PSH02-93) [NBP3-32519] - Western blot analysis of Lamin B Receptor on different lysates with Rabbit anti-Lamin B Receptor antibody (NBP3-32519) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 71 kDa Observed band size: 58 kDa Exposure time: Lane 1-2: 1 minute 2 seconds; Lane 3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32519) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Lamin B Receptor Antibody (PSH02-93) [NBP3-32519] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Lamin B Receptor antibody (NBP3-32519) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32519) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Lamin B Receptor Antibody (PSH02-93) [NBP3-32519] - Immunocytochemistry analysis of HEK-293 cells labeling Lamin B Receptor with Rabbit anti-Lamin B Receptor antibody (NBP3-32519) at 1/2,000 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lamin B Receptor antibody (NBP3-32519) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: Lamin B Receptor Antibody (PSH02-93) [NBP3-32519] - Flow cytometric analysis of HEK-293 cells labeling Lamin B Receptor. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32519, 1:1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: PSH02-93
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Lamin B Receptor Antibody (PSH02-93) Summary

• Description: Novus Biologicals Rabbit Lamin B Receptor Antibody (PSH02-93) (NBP3-32519) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within human Lamin B Receptor aa 1-500 / 615. (Uniprot: Q14739)
• Localization: Nucleus inner membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: LBR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-10000
  • Western Blot 1:2000
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Lamin B Receptor Antibody (PSH02-93)

• DHCR14B
• FLJ43126
• Integral nuclear envelope inner membrane protein
• lamin B receptor
• lamin-B receptor
• LMN2R
• MGC9041
• PHA

Background

Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization (1). Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures (2). The cleavage of lamins results in nuclear disregulation and cell death (3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LBR