![Western Blot: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Analysis of whole cell lysate from human (Hela) and mouse (C2C12, immortalized mouse adult fibroblasts (MAFs), NIE-115) cell lines were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and blotted with BB2SS3F3 antibody. Both primary and secondary antibodies were diluted in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). The predicted molecular weight is 70.7kDA and 71.4kDa for human and mouse LBR respectively. The absence of a band in LBR null MAFs indicated that the antibody specifically recognizes LBR.](http://images.novusbio.com/fullsize2/Lamin-B-Receptor-Antibody-(BB2SS3F3)-Western-Blot-NBP2-59947-img0001.jpg "Western Blot: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Analysis of whole cell lysate from human (Hela) and mouse (C2C12, immortalized mouse adult fibroblasts (MAFs), NIE-115) cell lines were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and blotted with BB2SS3F3 antibody. Both primary and secondary antibodies were diluted in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). The predicted molecular weight is 70.7kDA and 71.4kDa for human and mouse LBR respectively. The absence of a band in LBR null MAFs indicated that the antibody specifically recognizes LBR.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clone | BB2SS3F3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | GST fused to N-terminal fragment of human LBR (amino acids residues 1-211) |
| Specificity | Specificity of human, mouse Lamin B Receptor antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LBR |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IHC: paraffin-embedded section, cryosections. ICC/IF: formaldehyde, methanol fixation with methanol fixation resulting in a brighter signal. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.2, containing 40% glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-Frozen-NBP1-19371-img0013.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/CD4-Antibody-Flow-Cytometry-NBP1-19371-img0015.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Staining of human duodenum shows low expression as expected.](http://images.novusbio.com/images2/HPD-Antibody-Immunohistochemistry-Paraffin-NBP1-89366-img0007.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Staining of human liver shows high expression.](http://images.novusbio.com/images2/HPD-Antibody-Immunohistochemistry-Paraffin-NBP1-89366-img0008.jpg)
![Immunohistochemistry-Paraffin: HPD Antibody [NBP1-89366] - Staining in human liver and duodenum tissues using anti-HPD antibody. Corresponding HPD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/HPD-Antibody-Immunohistochemistry-Paraffin-NBP1-89366-img0009.jpg)
![Western Blot: VSX1 Antibody [NBP3-10377] - Western blot analysis using NBP3-10377 on Human Liver as a positive control. Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/images2/VSX1 Antibody-Western Blot-NBP3-10377-img0001.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-Paraffin-NBP1-49045-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0006.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-Frozen-NBP1-49045-img0008.jpg)
![Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Protein-Array-NBP2-79843-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79843-img0002.jpg)
![Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Western-Blot-NBP2-79843-img0003.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0014.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0015.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Lamin B Receptor Antibody (NBP2-59947)Discover more about diseases related to Lamin B Receptor Antibody (NBP2-59947).
| Pathways for Lamin B Receptor Antibody (NBP2-59947)View related products by pathway.
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PTMs for Lamin B Receptor Antibody (NBP2-59947)Learn more about PTMs related to Lamin B Receptor Antibody (NBP2-59947).
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| Gene Symbol | LBR |
![Immunocytochemistry/Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - The antibody specifically detects LBR. Wild-type immortalized mouse adult fibroblast (MAFs LBR+/+) (upper panel) and LBR-null MEFs (MAFs LBR-/-) (lower panel) were methanol fixed and immunofluorescently stained with BB2SS3F3 mouse monoclonal antibody. In LBR-null MEFs there is a loss of BB2SS3F3 antibody staining signal indicating the LBR specific signal of the BB2SS3F3 antibody.](http://images.novusbio.com/fullsize2/Lamin-B-Receptor-Antibody-(BB2SS3F3)-Immunocytochemistry-Immunofluorescence-NBP2-59947-img0004.jpg "Immunocytochemistry/Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - The antibody specifically detects LBR. Wild-type immortalized mouse adult fibroblast (MAFs LBR+/+) (upper panel) and LBR-null MEFs (MAFs LBR-/-) (lower panel) were methanol fixed and immunofluorescently stained with BB2SS3F3 mouse monoclonal antibody. In LBR-null MEFs there is a loss of BB2SS3F3 antibody staining signal indicating the LBR specific signal of the BB2SS3F3 antibody.")
![Immunocytochemistry/Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Staining of mouse small intestine. Small intestine from adult C57BL/6 mice was transferred to OCT compound and frozen in liquid nitrogen. Tissue cryosections were fixed in 10% neutral buffered formalin, permeabilized and stained with anti-LBR BB2SS3F3 antibody.](http://images.novusbio.com/fullsize2/Lamin-B-Receptor-Antibody-(BB2SS3F3)-Immunocytochemistry-Immunofluorescence-NBP2-59947-img0002.jpg "Immunocytochemistry/Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Staining of mouse small intestine. Small intestine from adult C57BL/6 mice was transferred to OCT compound and frozen in liquid nitrogen. Tissue cryosections were fixed in 10% neutral buffered formalin, permeabilized and stained with anti-LBR BB2SS3F3 antibody.")
![Immunocytochemistry/Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Confocal images of human (Hela) and mouse (NIE-115) cell lines immunofluorescently stained with BB2SS3F3 antibody. The BB2SS3F3 antibody works on cells fixed with 4% PFA and cold methanol with methanol fixation resulting in brighter signal of the antibody.](http://images.novusbio.com/fullsize2/Lamin-B-Receptor-Antibody-(BB2SS3F3)-Immunocytochemistry-Immunofluorescence-NBP2-59947-img0003.jpg "Immunocytochemistry/Immunofluorescence: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Confocal images of human (Hela) and mouse (NIE-115) cell lines immunofluorescently stained with BB2SS3F3 antibody. The BB2SS3F3 antibody works on cells fixed with 4% PFA and cold methanol with methanol fixation resulting in brighter signal of the antibody.")
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the PE/Cy5 conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (open histogram) or Anti-Human CD2 PE-Cy5 (filled histogram). Total cells were used for analysis.](http://images.novusbio.com/images2/CD2-Antibody-(RPA-2.10)-Flow-Cytometry-NBP2-25200-img0012.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP2-25200. Staining of CD2 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD2.](http://images.novusbio.com/images2/CD2-Antibody-(RPA-2.10)-Flow-Cytometry-NBP2-25200-img0008.jpg)
![Flow Cytometry: CD2 Antibody (RPA-2.10) [NBP2-25200] - Analysis using the Allophycocyanin conjugate of NBP2-25200. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (open histogram) or Anti-Human CD2 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/images2/CD2-Antibody-(RPA-2.10)-Flow-Cytometry-NBP2-25200-img0009.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/images2/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-(P3.6.2.8.1)-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")