Lamin B Receptor Antibody (BB2SS3F3) [Janelia Fluor® 525]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: BB2SS3F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

Lamin B Receptor Antibody (BB2SS3F3) [Janelia Fluor® 525] Summary

• Immunogen: GST fused to N-terminal fragment of human Lamin B Receptor (amino acids residues 1-211)
• Specificity: Specificity of human, mouse Lamin B Receptor antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LBR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Lamin B Receptor Antibody (BB2SS3F3) [Janelia Fluor® 525]

• DHCR14B
• FLJ43126
• Integral nuclear envelope inner membrane protein
• lamin B receptor
• lamin-B receptor
• LMN2R
• MGC9041
• PHA

Background

Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization (1). Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures (2). The cleavage of lamins results in nuclear disregulation and cell death (3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LBR