Recombinant Human Lactate Dehydrogenase A/LDHA His Protein

SDS-Page: Recombinant Human Lactate Dehydrogenase A/LDHA His Protein [NBP1-40407] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Discontinued

Recombinant Human Lactate Dehydrogenase A/LDHA His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-332 of Human Lactate Dehydrogenase A/LDHA
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF

• Details of Functionality: Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: LDHA
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 38.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Lactate Dehydrogenase A/LDHA His Protein

• Cell proliferation-inducing gene 19 protein
• EC 1.1.1
• EC 1.1.1.27
• GSD11
• Lactate Dehydrogenase A
• lactate dehydrogenase M
• LDH muscle subunit
• LDH1
• LDHA
• LDH-A
• LDHM
• LDH-M
• L-lactate dehydrogenase A chain
• proliferation-inducing gene 19
• Renal carcinoma antigen NY-REN-59

Background

LDHA, also known as L-lactate dehydrogenase A chain, is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. Recombinant LDHA protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Publications for Lactate Dehydrogenase A/LDHA Recombinant Protein (NBP1-40407)(1)

Publication using NBP1-40407

• Osis G, Traylor AM, Black LM et al. Expression of Lactate Dehydrogenase A and B isoforms in the mouse kidney American journal of physiology. Renal physiology 2021-03-15 [PMID: 33719570]

Bioinformatics

• Gene Symbol: LDHA