KMT2A/MLL Antibody - BSA Free

Western Blot: KMT2A/MLL Antibody [NB600-249] - Samples: Nuclear Extract (15 and 50 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: KMT2A/MLL Antibody [NB600-249] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-249 used for IP at 6 ug per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody NB600-248. For blotting immunoprecipitated MLL1, NB600-249 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

KMT2A/MLL Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit KMT2A/MLL Antibody - BSA Free (NB600-249) is a polyclonal antibody validated for use in WB and IP. Anti-KMT2A/MLL Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1320 and 1380 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KMT2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KMT2A/MLL Antibody - BSA Free

• ALL1
• ALL-1MLL/GAS7
• CXXC7TET1-MLL
• CXXC-type zinc finger protein 7
• EC 2.1.1.43
• HRXFLJ11783
• HTRX
• HTRX1MLL-AF4 der(11) fusion protein
• KMT2ACDK6/MLL fusion protein
• Lysine N-methyltransferase 2A
• MLL/GAS7 fusion protein
• MLL/GMPS fusion protein
• MLL1
• MLL1A
• myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog)
• myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
• Trithorax-like protein
• TRX1histone-lysine N-methyltransferase MLL
• Zinc finger protein HRX

Background

A partial tandem duplication within the MLL-gene (MLL-PTD) can be found in 8% of all patients with karyotypically normal acute myeloid leukemia (AML). MLL possibly acts as a transcriptional regulatory factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KMT2A/MLL Antibody (NB600-249)(1)

Publication using NB600-249

• Rampalli S, Li L, Mak E et al. p38 MAPK signaling regulates recruitment of Ash2L-containing methyltransferase complexes to specific genes during differentiation. Nat Struct Mol Biol 2007-12-01 [PMID: 18026121]

Bioinformatics

• Gene Symbol: KMT2A
• Entrez:
  • 4297(Human)
  • 214162(Mouse)
• Uniprot:
  • Q03164(Human)