KMT2A/MLL Antibody

Images

 
Biological Strategies: Western Blot: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear extract (50 and 15 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB ...read more
Immunoprecipitation: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-248 used ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

KMT2A/MLL Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 720 and 780 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MLL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunoprecipitation 2 - 10 ug/mg of lysate
Reviewed Applications
Read 1 Review rated 4
using
NB600-248 in the following applications:

Publications
Read Publications using NB600-248.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KMT2A/MLL Antibody

  • ALL1
  • ALL-1MLL/GAS7
  • CXXC7TET1-MLL
  • CXXC-type zinc finger protein 7
  • EC 2.1.1.43
  • HRXFLJ11783
  • HTRX
  • HTRX1MLL-AF4 der(11) fusion protein
  • KMT2ACDK6/MLL fusion protein
  • Lysine N-methyltransferase 2A
  • MLL/GAS7 fusion protein
  • MLL/GMPS fusion protein
  • MLL1
  • MLL1A
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog)
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
  • Trithorax-like protein
  • TRX1histone-lysine N-methyltransferase MLL
  • Zinc finger protein HRX

Background

A partial tandem duplication within the MLL-gene (MLL-PTD) can be found in 8% of all patients with karyotypically normal acute myeloid leukemia (AML). MLL possibly acts as a transcriptional regulatory factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-52983
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-28728
Species: Hu
Applications: IP (-), WB, IHC
NBP2-42210
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP1-89105
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-864
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
AF3254
Species: Hu, Mu, Rt
Applications: WB
AF5414
Species: Hu
Applications: WB, Simple Western
NBP1-30141
Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
NB100-1566
Species: Hu
Applications: WB, IP
NBP1-26653
Species: Hu
Applications: WB, IHC, IHC-P, IP
AF5129
Species: Hu
Applications: WB
NB100-2628
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
NBP1-80695
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45545
Species: Hu
Applications: WB, IHC
NBP2-65123
Species: Hu
NBP2-20619
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-381
Species: Hu
Applications: WB, ChIP, IP
NB110-61646
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-215
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NB600-248
Species: Hu
Applications: WB, IP

Publications for KMT2A/MLL Antibody (NB600-248)(25)

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB600-248 Applications Species
Barrett NA, Malouf C, Kapeni C et al. Mll-AF4 Confers Enhanced Self-Renewal and Lymphoid Potential during a Restricted Window in Development Cell Rep 2016 Jul 26 [PMID: 27396339] (IHC-P) IHC-P
Shore AN, Kabotyanski EB, Roarty K et al. Pregnancy-induced noncoding RNA (PINC) associates with polycomb repressive complex 2 and regulates mammary epithelial differentiation. PLoS Genet 2012 [PMID: 22911650]
Liu X, Qu X, Chen Y et al. Mesenchymal stem/stromal cells induce the generation of novel IL-10-dependent regulatory dendritic cells by SOCS3 activation. J Immunol 2012 Aug [PMID: 22753940]
Akalin A, Garrett-Bakelman FE, Kormaksson M et al. Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia. PLoS Genet 2012 [PMID: 22737091]
Zhang Y, Liu Z, Medrzycki M et al. Reduction of Hox gene expression by histone H1 depletion. PLoS One 2012 [PMID: 22701719]
Wang QF, Wu G, Mi S et al. MLL fusion proteins preferentially regulate a subset of wild-type MLL target genes in the leukemic genome. Blood 2011 Jun [PMID: 21518926]
Chen et al. Mol.Cell.Biol. 30 (24):5621-5635. 2010 [PMID: 20937768]
Lee et al. J.Biol.Chem 285 (32):24466-24476., 2010. [PMID: 20516061]
Gialitakis et al. Mol.Cell.Biol. 30 (8):2046-2056. 2010 [PMID: 20123968]
Horiguchi et al. Clin Cancer Res 15 (8):2620-2629. 2009 [PMID: 19318494]
Show All 25 Publications.

Review for KMT2A/MLL Antibody (NB600-248) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-248:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot KMT2A/MLL NB600-248
Enlarge
reviewed by:
Anonymous
WB Human 08/27/2014
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate from Jurkat cells grown in RPMI medium
SpeciesHuman
CommentsThe antibody gives multiple bands, probably degraded products of MLL protein.

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:2000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 30sec

Comments

CommentsThe antibody gives multiple bands, probably degraded products of MLL protein.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for KMT2A/MLL Antibody (NB600-248) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for KMT2A/MLL Antibody (NB600-248)

Discover related pathways, diseases and genes to KMT2A/MLL Antibody (NB600-248). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for KMT2A/MLL Antibody (NB600-248)

Discover more about diseases related to KMT2A/MLL Antibody (NB600-248).
 

Pathways for KMT2A/MLL Antibody (NB600-248)

View related products by pathway.

PTMs for KMT2A/MLL Antibody (NB600-248)

Learn more about PTMs related to KMT2A/MLL Antibody (NB600-248).

Blogs on KMT2A/MLL

There are no specific blogs for KMT2A/MLL, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
08/27/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MLL
Entrez
Uniprot