Klotho beta Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Klotho beta Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Klotho beta
  Phe53-Leu995
  Accession # NP_112457
• Specificity: Detects mouse Klotho beta in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse Klotho is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KLB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Klotho beta Antibody [Biotin]

• betaKlotho
• beta-klotho
• BKL
• KLB
• klotho beta like
• Klotho beta
• klotho beta-like protein
• MGC142213

Background

Klotho beta , a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex (2). The Klotho beta mediated interaction of FGF15 (human FGF19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2-4). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake (2, 3, 5). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 6). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible (7). The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho beta , respectively. The low identity with rat reflects aa discordance within rodent ECD.

1. Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
2. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
3. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
4. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
5. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
6. Chang, Q. et al. (2005) Science 310:490.
7. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Klotho beta Antibody (BAF2619)(1)

Publication using BAF2619

• Diaz-Delfin J, Hondares E, Iglesias R, Giralt M, Caelles C, Villarroya F TNF-alpha represses beta-Klotho expression and impairs FGF21 action in adipose cells: involvement of JNK1 in the FGF21 pathway. Endocrinology, 2012-07-09;153(9):4238-45. 2012-07-09 [PMID: 22778214] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: KLB
• Uniprot:
  • NP_112457()