KLF8 Antibody

Western Blot: KLF8 Antibody [NBP2-27418] - Analysis of KLF8 using KLF8 antibody. Human Kidney tissue lysate in the 1) absence and 2) presence of immunizing peptide and 3) HEK293 lysate probed with 2 ug/ml of KLF8 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Pm, Pm-Or
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KLF8 Antibody Summary

• Description: Novus Biologicals Rabbit KLF8 Antibody (NBP2-27418) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 1-50 from human kLF8 was used as the immunogen for this antibody.
• Predicted Species: Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KLF8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4ug/ml

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in White-cheeked Gibbon, 89% in Rhesus Macaque.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for KLF8 Antibody

• Basic krueppel-like factor 3
• basic kruppel-like factor 3
• BKLF3DKFZp686O08126
• DXS741
• Krueppel-like factor 8
• Kruppel-like factor 8
• Zinc finger protein 741
• ZNF741MGC138314

Background

kLF8, kruppel-like factor 8, is a 40kDa oncoprotein linked to activity of Src, FAk, PI3has k, cyclin D1, and SP1, among other factors shown in the development of cancers (1-3). As other kLF proteins, kLF8 has been associated with oncogenic pathways and elevated expression of kFL8 has been shown in human cancer cells as well as in primary tumor tissues (4). kLF8 also appears to be associated with differentiation not only of cancer cells but stem cells as well as a transcriptional repressor and activator. Specific mechanisms of kLF8 regulation of oncogenic pathways or in stem cell differentiation pathways remain to be clarified, making this kLF8 specific antibody an enabling tool for such research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLF8
• Uniprot:
  • O95600(Human)