KLF2 Antibody (665333)

Images

 
KLF2 was detected in immersion fixed BG01V human embryonic stem cells using Human KLF2 Monoclonal Antibody (Catalog # MAB5466) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 Monoclonal Antibody (Catalog # MAB5466, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clone
665333
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

KLF2 Antibody (665333) Summary

Immunogen
E. coli-derived recombinant human KLF2
Pro71-Pro168 (predicted)
Accession # Q9Y5W3
Specificity
Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross-reactivity with rhKLF1, 4, 5, 6, 10, 12, 17, recombinant mouse KLF4 or 15 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
KLF2
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB5466 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLF2 Antibody (665333)

  • KLF2
  • Krueppel-like factor 2
  • Kruppel-like factor 2 (lung)
  • Kruppel-like factor LKLF
  • LKLF
  • LKLFLung krueppel-like factor
  • lung Kruppel-like zinc finger transcription factor

Background

KLF2 (Kruppel-like factor 2; also LKLF) is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family.  KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells.  It is a transcription factor that regulates multiple genes, many of which are involved in cell migration.   Human KLF2 is 355 amino acids (aa) in length.  It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354).  There is one potential splice form that shows a premature truncation after Asp224.  Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF2 Antibody (MAB5466)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


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ICC
(1)
Western Blot
(1)
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Filter By Species
Human
(2)
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FAQs for KLF2 Antibody (MAB5466) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on KLF2.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

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Bioinformatics

Gene Symbol KLF2
Uniprot