KLF15 Antibody - BSA Free

Western Blot: KLF15 Antibody [NBP2-24635] - Analysis of KLF15 in human liver lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse liver lysate using NBP2-24635 at 1.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunocytochemistry/Immunofluorescence: KLF15 Antibody [NBP2-24635] - KLF15 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: KLF15 Antibody [NBP2-24635] - Tissue section of human liver using KLF15 antibody at 1:100 dilution. The antibody generated strong cytoplasmic staining with some nuclear signal in the hepatocytes, while in Kupffer cells, the signal was mainly nuclear and was most intense among all cell types.

• Reactivity: Hu, Mu, Bv, Rt, Po
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

KLF15 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit KLF15 Antibody - BSA Free (NBP2-24635) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-KLF15 Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an internal portion of the human KLF15 protein (between amino acids 100-200) [UniProt Q9UIH9]
• Localization: Nucleus, Cytoplasm
• Predicted Species: Rat (90%), Porcine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KLF15
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2-5 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Western Blot 1.0-2.0 ug/ml

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 29551501). Use in Mouse reported in scientific literature (PMID:32437957).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for KLF15 Antibody - BSA Free

• DKFZp779M1320
• kidney-enriched Kruppel-like factor
• KKLFKidney-enriched krueppel-like factor
• Krueppel-like factor 15
• Kruppel-like factor 15

Background

The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KLF15 Antibody (NBP2-24635)(9)

Publications using NBP2-24635

• Hashimoto K, Kodama A, Ohira M et al. Postnatal expression of cell cycle promoter Fam64a causes heart dysfunction by inhibiting cardiomyocyte differentiation through repression of Klf15 iScience 2022-04-01 [PMID: 35602953] (WB, Mouse)
• Rivera ME, Vaughan RA Comparing the effects of palmitate, insulin, and palmitate-insulin co-treatment on myotube metabolism and insulin resistance Lipids 2021-08-12 [PMID: 34382222]
• Biswas D, Tozer K, Dao K et al. Adverse Outcomes in Obese Cardiac Surgery Patients Correlates With Altered Branched-Chain Amino Acid Catabolism in Adipose Tissue and Heart Front. Endocrinol. 2020-08-07 [PMID: 32903728] (Human)
• Trivedi PC, Bartlett JJ, Mercer A et al. Loss of function of transcription factor EB remodels lipid metabolism and cell death pathways in the cardiomyocyte Biochim Biophys Acta Mol Basis Dis 2020-05-08 [PMID: 32437957] (WB, Mouse)
• Rivera ME, Lyon ES, Vaughan RA Effect of metformin on myotube BCAA catabolism J. Cell. Biochem. 2019-08-05 [PMID: 31385363]
• Guo H, Khan R, Raza SHA et al. KLF15 promotes transcription of KLF3 gene in bovine adipocytes. Gene. 2018-03-15 [PMID: 29551501] (Bovine)
• Pandey D, Nomura Y, Rossberg MC, Hori D. Hypoxia Triggers SENP1 (Sentrin-Specific Protease 1) Modulation of KLF15 (Kruppel-Like Factor 15) and Transcriptional Regulation of Arg2 (Arginase 2) in Pulmonary Endothelium. Arterioscler. Thromb. Vasc. Biol. 2018-02-22 [PMID: 29472234] (WB, Human)
• Trivedi P ROLE OF TRANSCRIPTION FACTOR EB (TFEB) IN CARDIAC HEALTH AND DISEASE Thesis 2020-01-01 (WB, Rat)
• Chen X, Raza SHA, Cheng G et al. Bta-miR-376a Targeting KLF15 Interferes with Adipogenesis Signaling Pathway to Promote Differentiation of Qinchuan Beef Cattle Preadipocytes Animals : an open access journal from MDPI 2020-12-10 [PMID: 33321855] (WB, Bovine)

Bioinformatics

• Gene Symbol: KLF15
• Entrez:
  • 28999(Human)
• Uniprot:
  • Q9UIH9(Human)