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KLF15 Antibody

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Western Blot: KLF15 Antibody [NBP2-24635] - Analysis of KLF15 in human liver lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse liver lysate using NBP2-24635 at 1.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: KLF15 Antibody [NBP2-24635] - Analysis of KLF15 in human liver lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse liver lysate using NBP2-24635 at 1.0 ug/ml. Goat anti-rabbit Ig ...read more
Immunocytochemistry/ Immunofluorescence: KLF15 Antibody [NBP2-24635] - KLF15 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: KLF15 Antibody [NBP2-24635] - Tissue section of human liver using KLF15 antibody at 1:100 dilution. The antibody generated strong cytoplasmic staining with some nuclear signal in the ...read more

Product Details

Summary
Reactivity Hu, Bv, Mu, Rt, PoSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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KLF15 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human KLF15 protein (between amino acids 100-200) [UniProt Q9UIH9]
Localization
Nucleus, Cytoplasm
Predicted Species
Mouse (95%), Rat (90%), Porcine (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KLF15
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1.0-2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2-5 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml
Publications
Read Publications using
NBP2-24635 in the following applications:

  • WB
    1 publication

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 29551501).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for KLF15 Antibody

  • DKFZp779M1320
  • kidney-enriched Kruppel-like factor
  • KKLFKidney-enriched krueppel-like factor
  • Krueppel-like factor 15
  • Kruppel-like factor 15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
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Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.
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Western blot shows lysates of BG01V human embryonic stem cells, D3 mouse embryonic stem cell line, and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human KLF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for KLF5 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Publications for KLF15 Antibody (NBP2-24635)(3)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 1 application: WB.


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WB Video Protocol
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Bioinformatics

Gene Symbol KLF15
Entrez
Uniprot
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