NRL Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

NRL Antibody Summary

• Immunogen: E. coli-derived recombinant human NRL
  Met1-Leu237
  Accession # P54845
• Specificity: Detects human NRL in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRL
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NRL Antibody

• D14S46Eneural retina-specific leucine zipper protein
• neural retina leucine zipper
• NRL
• NRL-MAF
• RP27
• RP27neural retinal-specific leucine zipper

Background

NRL is a 30 kDa nuclear transcription factor that belongs to the Maf subfamily within the bZIP (basic motif-leucine zipper) transcription factor family. It contains an N‑terminal transcriptional activation domain and a C-terminal DNA-binding bZIP domain that is also involved in homo- or heterodimerization. NRL regulates the expression of rod-specific genes and is expressed specifically in rod photoreceptors. NRL is phosphorylated at multiple sites, which likely regulates its activity. The amino acid sequence of human NRL is 92%, 94%, 90% and 90% identical to that of bovine, canine, rat and mouse NRL, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NRL Antibody (AF2945)(5)

Publications using AF2945

• LR Bohrer, NE Stone, NK Mullin, AP Voigt, KR Anfinson, JL Fick, V Luangphakd, B Hittle, K Powell, GF Muschler, RF Mullins, EM Stone, BA Tucker Automating iPSC generation to enable autologous photoreceptor cell replacement therapy Journal of Translational Medicine, 2023-02-28;21(1):161. 2023-02-28 [PMID: 36855199] (IHC, Human)
• IC Han, LR Bohrer, KN Gibson-Cor, LA Wiley, A Shrestha, BE Harman, C Jiao, EH Sohn, R Wendland, BN Allen, KS Worthingto, RF Mullins, EM Stone, BA Tucker Biocompatibility of Human Induced Pluripotent Stem Cell-Derived Retinal Progenitor Cell Grafts in Immunocompromised Rats Cell Transplantation, 2022-01-01;31(0):9636897221104. 2022-01-01 [PMID: 35758274] (ICC, Human)
• S Yamasaki, A Kuwahara, A Kishino, T Kimura, M Takahashi, M Mandai Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture Regenerative Therapy, 2022-01-01;19(0):24-34. 2022-01-01 [PMID: 35059477] (IHC, Human)
• A Kuwahara, S Yamasaki, M Mandai, K Watari, K Matsushita, M Fujiwara, Y Hori, Y Hiramine, D Nukaya, M Iwata, A Kishino, M Takahashi, Y Sasai, T Kimura Preconditioning the Initial State of Feeder-free Human Pluripotent Stem Cells Promotes Self-formation of Three-dimensional Retinal Tissue Sci Rep, 2019-12-12;9(1):18936. 2019-12-12 [PMID: 31831759] (IHC-Fr, Human, Xenograft)
• Gonzalez-Cordero A, Kruczek K, Naeem A, Fernando M, Kloc M, Ribeiro J, Goh D, Duran Y, Blackford S, Abelleira-Hervas L, Sampson R, Shum I, Branch M, Gardner P, Sowden J, Bainbridge J, Smith A, West E, Pearson R, Ali R Recapitulation of Human Retinal Development from Human Pluripotent Stem Cells Generates Transplantable Populations of Cone Photoreceptors. Stem Cell Reports, 2017-08-24;9(3):820-837. 2017-08-24 [PMID: 28844659] (IHC, Human)

Bioinformatics

• Gene Symbol: NRL
• Uniprot:
  • P54845()