KISS1 Antibody (1F7) - Azide and BSA Free

Western Blot: KISS1 Antibody (1F7) [H00003814-M05] - Analysis of KISS1 expression in transfected 293T cell line by KISS1 monoclonal antibody (M05), clone 1F7. Lane 1: KISS1 transfected lysatE (14.7 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: KISS1 Antibody (1F7) [H00003814-M05] - Analysis of monoclonal antibody to KISS1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml

ELISA: KISS1 Antibody (1F7) [H00003814-M05] - Detection limit for recombinant GST tagged KISS1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

KISS1 Antibody (1F7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: KISS1 (NP_002247, 46 a.a. ~ 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGEQSLPCTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRSAGRGWGAGAGQ
• Specificity: KISS1 - KiSS-1 metastasis-suppressor
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KISS1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KISS1 Antibody (1F7) - Azide and BSA Free

• KiSS-1 metastasis-suppressor
• KiSS1
• KiSS-1
• kisspeptin
• Kisspeptin-1
• malignant melanoma metastasis-suppressor
• metastasis-suppressor KiSS-1
• Metastin
• MGC39258

Background

This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KISS1 Antibody (H00003814-M05)(7)

Publications using H00003814-M05

• Shin WJ, Cho YA, Kang KR et al. KiSS-1 expression in oral squamous cell carcinoma and its prognostic significance. APMIS 2016-01-25 [PMID: 26809635]
• Sitticharoon C, Boonpuan V, Mitrpant C, Churintaraphan M. Determination of KISS1, KISS1R and Kisspeptin in Fat Tissue of Normal Weight and Obese Humans and Correlations between Serum Kisspeptin and Leptin. Siriraj Med J 2013-01-01 [PMID: 26161358]
• Kang HS, Baba T, Mandai M et al. GPR54 is a target for suppression of metastasis in endometrial cancer. Mol Cancer Ther. 2011-01-31 [PMID: 21282360]
• Martins CM, Fernandes BF, Antecka E et al. Expression of the metastasis suppressor gene KISS1 in uveal melanoma. Eye. 2008-01-25 [PMID: 18219339]
• Sitticharoon C, Boonpuan V, Mitrpant C et al. Determination of KISS1, KISS1R and Kisspeptin in Fat Tissue of Normal Weight and Obese Humans and Correlations between Serum Kisspeptin and Leptin. Siriraj medical journal 2013-04-02 (ICC/IF, Human)
• Zhu W, Gao A, Chen X et al. Expression of KiSS-1 in Epithelial Ovarian Cancer and its Role in Metastasis. Applied Mechanics and Materials 2011-01-01
• Baba Tsukasa, Kang Hyun Sook, Hosoe Yuko et al. Menstrual cyclic change of metastin/GPR54 in endometrium. Med Mol Morphol. 2014-06-08 [PMID: 24908069]

Bioinformatics

• Gene Symbol: KISS1
• Entrez:
  • 3814(Human)
• OMIM:
  • 603286(Human)
• Uniprot:
  • NP_002247(Human)