Kinesin 5C Antibody - BSA Free

Western Blot: Kinesin 5C Antibody [NBP1-87429] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunohistochemistry-Paraffin: Kinesin 5C Antibody [NBP1-87429] - Staining of human prostate shows weak cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Kinesin 5C Antibody [NBP1-87429] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells and weak staining of neuropil.

Immunohistochemistry-Paraffin: Kinesin 5C Antibody [NBP1-87429] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: Kinesin 5C Antibody [NBP1-87429] - Analysis in human cerebral cortex and liver tissues using NBP1-87429 antibody. Corresponding KIF5C RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Kinesin 5C Antibody [NBP1-87429] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: Kinesin 5C Antibody [NBP1-87429] - Staining of human testis shows weak cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Kinesin 5C Antibody [NBP1-87429] - Staining of human liver shows no positivity in hepatocytes as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Kinesin 5C Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EVEDKTRANEQLTDELAQKTTTLTTTQRELSQLQELSNHQKKRATEILNLLLKDLGEIGGIIGTNDVKTLADVNGV
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF5C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Kinesin 5C Protein (NBP1-87429PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Kinesin 5C Antibody - BSA Free

• heavy chain, neuron-specific
• KIF 5C
• kinesin family member 5C
• Kinesin heavy chain neuron-specific 2
• MGC111478
• NKHC
• NKHC2NKHC-2

Background

Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIF5C