Casein Kinase 2 alpha Antibody (844720) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, C6 rat glioma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Casein Kinase 2a Monoclonal Antibody (Catalog # MAB7957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Casein Kinase 2a at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Casein Kinase 2a was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Casein Kinase 2a Monoclonal Antibody (Catalog # MAB7957) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.5 mg/mL. A specific band was detected for Casein Kinase 2 alpha at approximately 50-52 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) . This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Casein Kinase 2a was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Casein Kinase 2a Monoclonal Antibody (Catalog # MAB7957) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of HAP1 human colorectal carcinoma cell line and Casein Kinase 2 alpha knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with 0.5 µg/mL of Mouse Anti-Human Casein Kinase 2a Monoclonal Antibody (Catalog # MAB7957) followed by HRP-conjugated goat anti-mouse IgG Secondary Antibody. A specific band was detected for Casein Kinase 2 alpha at approximately 47 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

HAP1 WT and Casein Kinase 2 alpha KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) followed by incubation with a goat anti-mouse Alexa-fluor 555 coupled secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 1 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF, KO
• Clone: 844720
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Casein Kinase 2 alpha Antibody (844720) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Casein Kinase 2 alpha
  Asp253-Gln391
  Accession # P68400
• Specificity: Detects human Casein Kinase 2 alpha in ELISAs and Western blots. Detects mouse and rat Casein Kinase 2 alpha in Western blots
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSNK2A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Casein Kinase 2 alpha Antibody (844720) [Unconjugated]

• Casein Kinase 2 alpha
• casein kinase 2, alpha 1 polypeptide
• casein kinase II alpha 1 subunit
• CK II alpha
• CK2 catalytic subunit alpha
• CK2A1
• CK2A1casein kinase II subunit alpha
• CKII
• CSNK2A1
• EC 2.7.11
• EC 2.7.11.1
• protein kinase CK2

Background

Casein kinase 2 (CK2) is a ubiquitous and constitutively active tetrameric serine/threonine kinase that is comprised of two catalytic subunits (CK2 alpha and/or CK2 alpha ‘) and two identical regulatory subunits (CK2 beta ). CK2 has been implicated in numerous cellular processes, including signal transduction, transcription, translation, replication, and metabolic pathways. CK2 is known to phosphorylate more than 300 different substrates. Phosphorylation of cell-cycle proteins such as p53, p34cdc2, p27KIP1, and MDM-2 account for the ability of CK2 to induce proliferation, while the phosphorylation of HS1, Bid, and Max account for its antiapoptotic role. The human CK2 alpha and CK2 alpha ‘ subunits are the products of two different genes. They have highly conserved catyalytic domains but divergent C-terminal regions. Within aa 253-391 (including the region of divergence between CK2 alpha and CK2 alpha ’), the 35-45 kDa human CK2 alpha shares 96% aa sequence identity with mouse and rat CK2 alpha . An alternatively spliced isoform of human CK2 alpha lacks the N-terminal 136 amino acids including a portion of the kinase domain. CK2 beta plays dual roles in the regulation of CK2 acivity. Its C-terminal domain is responsible for stable interactions with the catalytic subunit and increased catalytic activity following tetramer formation, while the N-terminal domain exerts negative regulation on the catalytic activity of CK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Casein Kinase 2 alpha Antibody (MAB7957)(3)

Publications using MAB7957

• Ayoubi, R;Fotouhi, M;Alende, C;Ruíz Moleón, V;Southern, K;Laflamme, C;NeuroSGC/YCharOS/EDDU collaborative group, ;ABIF consortium, ; A guide to selecting high-performing antibodies for CSNK2A1 (UniProt ID: P68400) for use in western blot, immunoprecipitation and immunofluorescence F1000Research 2024-09-05 [PMID: 39403680] (Western Blot, Human)
• Yang Yang, Xin Fan, Yukai Ren, Kai Wu, Xiangyu Tian, Fengbiao Wen et al. SOX2-Upregulated microRNA-30e Promotes the Progression of Esophageal Cancer via Regulation of the USP4/SMAD4/CK2 Axis Molecular Therapy - Nucleic Acids 2021-03-05 [PMID: 33376627]
• Yang Yang, Xin Fan, Yukai Ren, Kai Wu, Xiangyu Tian, Fengbiao Wen et al. SOX2-Upregulated microRNA-30e Promotes the Progression of Esophageal Cancer via Regulation of the USP4/SMAD4/CK2 Axis Molecular Therapy - Nucleic Acids 2021-03-05 [PMID: 33376627] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CSNK2A1
• Uniprot:
  • P68400()