Casein Kinase 2 alpha Antibody (844720) [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line,MOLT‑4 human acute lymphoblastic leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, C6 rat glioma cell line, and ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.5 mg/mL. A specific band was detected for Casein Kinase 2 ...read more
Casein Kinase 2 alpha was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) at 25 µg/mL for 3 hours at room ...read more
Casein Kinase 2 alpha was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) at 5 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
844720
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Casein Kinase 2 alpha Antibody (844720) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Casein Kinase 2 alpha
Asp253-Gln391
Accession # P68400
Specificity
Detects human Casein Kinase 2 alpha in ELISAs and Western blots. Detects mouse and rat Casein Kinase 2 alpha in Western blots
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSNK2A1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Control
Casein Kinase 2 alpha Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Casein Kinase 2 alpha Antibody (844720) [Unconjugated]

  • Casein Kinase 2 alpha
  • casein kinase 2, alpha 1 polypeptide
  • casein kinase II alpha 1 subunit
  • CK II alpha
  • CK2 catalytic subunit alpha
  • CK2A1
  • CK2A1casein kinase II subunit alpha
  • CKII
  • CSNK2A1
  • EC 2.7.11
  • EC 2.7.11.1
  • protein kinase CK2

Background

Casein kinase 2 (CK2) is a ubiquitous and constitutively active tetrameric serine/threonine kinase that is comprised of two catalytic subunits (CK2 alpha and/or CK2 alpha ‘) and two identical regulatory subunits (CK2 beta ). CK2 has been implicated in numerous cellular processes, including signal transduction, transcription, translation, replication, and metabolic pathways. CK2 is known to phosphorylate more than 300 different substrates. Phosphorylation of cell-cycle proteins such as p53, p34cdc2, p27KIP1, and MDM-2 account for the ability of CK2 to induce proliferation, while the phosphorylation of HS1, Bid, and Max account for its antiapoptotic role. The human CK2 alpha and CK2 alpha ‘ subunits are the products of two different genes. They have highly conserved catyalytic domains but divergent C-terminal regions. Within aa 253-391 (including the region of divergence between CK2 alpha and CK2 alpha ’), the 35-45 kDa human CK2 alpha shares 96% aa sequence identity with mouse and rat CK2 alpha. An alternatively spliced isoform of human CK2 alpha lacks the N-terminal 136 amino acids including a portion of the kinase domain. CK2 beta plays dual roles in the regulation of CK2 acivity. Its C-terminal domain is responsible for stable interactions with the catalytic subunit and increased catalytic activity following tetramer formation, while the N-terminal domain exerts negative regulation on the catalytic activity of CK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CSNK2A1
Uniprot