Kinesin 5A Antibody

Images

 
Western Blot: Kinesin 5A Antibody [NBP1-76816] - K562 cell lysate with Kinesin 5A antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody [NBP1-76816] - Immunofluorescence of KIF5 in K562 cells with KIF5 antibody at 20 ug/mL.
Immunohistochemistry: Kinesin 5A Antibody [NBP1-76816] - Staining of KIF5 in mouse brain tissue with KIF5 antibody at 5 ug/ml.
Western Blot: Kinesin 5A Antibody [NBP1-76816] - Human samples tested and cleaned up bands after speaking with technical service and changing wash protocol. Image provided by verified customer review.
Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody [NBP1-76816] - K562 cells with Kinesin 5A antibody at 5 ug/ml.
Immunofluorescence: Kinesin 5A Antibody [NBP1-76816] - Immunofluorescence of Kinesin 5A in mouse brain tissue with Kinesin 5A antibody at 20 ug/mL. Red: Kinesin 5A Antibody (NBP1-76816), Blue: DAPI staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Kinesin 5A Antibody Summary

Immunogen
Antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human KIF5. The immunogen is located within amino acids 880 - 930 of KIF5.
Specificity
At least three isoforms of KIF5 are known to exist; this antibody will recognize all three isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIF5A
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunofluorescence
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Reviewed Applications
Read 1 Review rated 4
using
NBP1-76816 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Kinesin 5A Antibody

  • D12S1889
  • KIF5A variant protein
  • kinesin family member 5A
  • kinesin heavy chain isoform 5A
  • Kinesin heavy chain neuron-specific 1
  • kinesin, heavy chain, neuron-specific
  • MY050
  • Neuronal kinesin heavy chain
  • NKHC1
  • NKHCspastic paraplegia 10 (autosomal dominant)
  • SPG10

Background

The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kinesin 5A Antibody (NBP1-76816) (0)

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Review for Kinesin 5A Antibody (NBP1-76816) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-76816:
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Images Ratings Applications Species Date Details
Western Blot Kinesin 5A NBP1-76816
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reviewed by:
Anonymous
WB Human 02/28/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsAt first I had issues with background bands but after talking to customer service and changing my wash protocol the antibody worked very nicely.
FileView PDF

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ICC/IF Video Protocol

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Anonymous
02/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol KIF5A
Uniprot