Western Blot: Kinesin 5A Antibody [NBP1-76816] - K562 cell lysate with Kinesin 5A antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody [NBP1-76816] - Immunofluorescence of KIF5 in K562 cells with KIF5 antibody at 20 ug/mL.
Immunohistochemistry: Kinesin 5A Antibody [NBP1-76816] - Immunofluorescence of Kinesin 5A in mouse brain tissue with Kinesin 5A antibody at 20 ug/mL. Red: (NBP1-76816), Blue: DAPI staining.
Western Blot: Kinesin 5A Antibody [NBP1-76816] - Human samples tested and cleaned up bands after speaking with technical service and changing wash protocol. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Kinesin 5A Antibody [NBP1-76816] - K562 cells with Kinesin 5A antibody at 5 ug/ml.
Immunohistochemistry: Kinesin 5A Antibody [NBP1-76816] - Staining of KIF5 in mouse brain tissue with KIF5 antibody at 5 ug/ml.
Antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human KIF5. The immunogen is located within amino acids 880 - 930 of KIF5. Amino Acid Squence: KDKRRYQQEVDRIKEAVRYK
At least three isoforms of KIF5 are known to exist; this antibody will recognize all three isoforms.
Peptide affinity purified
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Kinesin 5A antibody validated for WB from a verified customer review.
116 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for Kinesin 5A Antibody
KIF5A variant protein
kinesin family member 5A
kinesin heavy chain isoform 5A
Kinesin heavy chain neuron-specific 1
kinesin, heavy chain, neuron-specific
Neuronal kinesin heavy chain
NKHCspastic paraplegia 10 (autosomal dominant)
The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.