Ki67/MKI67 Antibody

Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB500-170] - Tissue section of human tonsil using 1:50 dilution of rabbit anti-KI67 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Ki67 antibody generated a specific nuclear staining in the cells in germinal centers of the tested tonsil tissue.

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB500-170] - Ki67 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Immunohistochemistry: Ki67/MKI67 Antibody [NB500-170] - Senescent MSPCs are characterized by loss of nestin expression. Double-immunofluorescence images of femoral metaphysis sections from 4-, 6-, 8-, and 12-week-old male Nestin-GFP mice using antibodies against GFP (green) and Ki67 (red). DAPI stains nuclei blue. GP growth plate. PS primary spongiosa. Quantification of the percentage of GFP+ cells that express Ki67. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01509-0) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB500-170] - Ki-67/MKI67 Antibody [NB500-170] - Human tonsil using 1:200 dilution of rabbit anti-KI67 antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Ki67 antibody generated a specific nuclear staining in the cells in germinal centers of the tested tonsil tissue.

Western Blot: Ki67/MKI67 Antibody [NB500-170] - Ki-67/MKI67 Antibody [NB500-170] - Analysis of A431 (A) and Hek293 (B) cell lysate using Ki67 antibody (NB500-170) at 2 ug/ml.

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB500-170] - Confocal immunofluorescent analysis of MCF7 cells using Ki67 antibody (NB500-170, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB500-170] - Ki-67/MKI67 Antibody [NB500-170] - Human tonsil.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, IB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Ki67/MKI67 Antibody Summary

• Immunogen: The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Human KI67/MKI67, between amino acids: 1218-1232 1582-1596, 2310-2324 [Uniprot: P46013].
• Localization: Nuclear
• Marker: Proliferation Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MKI67
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:50-1:300
  • Immunohistochemistry-Frozen 1:50-1:300
  • Immunohistochemistry-Paraffin 1:50-1:300
• Application Notes: Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. This antibody will not work without optimal antigen retrieval, and is a critical step. NOTE: We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein. Ki67/MKI67 Antibody was used for IB (PMID: 24248265) and IHC-Fr (PMID: 22493503).
• Theoretical MW: 359 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Ki67/MKI67 Knockout HeLa Cell Lysate

Reactivity Notes

Ki67/MKI67 Antibody reacted with Mouse (PMID: 22033079), Rat (PMID: 22521325), and Porcine (PMID: 29146772).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ki67/MKI67 Antibody

• antigen identified by monoclonal Ki-67
• Antigen Ki67
• antigen KI-67
• antiKi67
• Ki67 ihc
• Ki-67 ihc
• Ki67 mouse
• Ki-67 mouse
• Ki67 western blot
• Ki-67 western blot
• Ki67
• Ki-67
• KIA
• Marker Of Proliferation Ki-67
• MIB-
• MIB-1
• MKI67
• PPP1R105
• Proliferation Marker Protein Ki-67
• proliferation-related Ki-67 antigen
• Protein Phosphatase 1
• Regulatory Subunit 105
• TSG126

Background

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ki67/MKI67 Antibody (NB500-170)(92)

Publications using NB500-170

• Sato N, Kumasawa K, Yamashita M et al. Therapeutic potential of combination therapy of soluble VEGF receptor 1 and conventional chemotherapy for ovarian cancer growth J. Obstet. Gynaecol. Res. Feb 5 2020 [PMID: 32022375]
• Zhou JY, Huang DG, Zhu M et al. Wnt/beta-catenin-mediated heat exposure inhibits intestinal epithelial cell proliferation and stem cell expansion through endoplasmic reticulum stress J. Cell. Physiol. Jan 21 2020 [PMID: 31960439]
• Jin CL, Ye JL, Yang J mTORC1 Mediates Lysine-Induced Satellite Cell Activation to Promote Skeletal Muscle Growth Cells Nov 30 2019 [PMID: 31801253] (IHC, Porcine)
• Zhao S, Zhong S, Wang F et al. Microcystin-LR exposure decreased the fetal weight of mice by disturbance of placental development and ROS-mediated endoplasmic reticulum stress in the placenta Environ. Pollut. 2019 Oct 11 [PMID: 31672369] (IHC, Mouse)
• Zhou JY, Wang Z, Zhang SW et al. Methionine and its Hydroxyl Analogs Improve Stem Cell Activity to Eliminate Deoxynivalenol-Induced Intestinal Injury by Reactivating Wnt/beta-catenin Signaling J. Agric. Food Chem. Sep 18 2019 [PMID: 31532211] (Mouse)
• Kinjo T, Ito M, Seki T et al. Prenatal exposure to valproic acid is associated with altered neurocognitive function and neurogenesis in the dentate gyrus of male offspring rats Brain Res. Aug 22 2019 [PMID: 31446017] (IHC-Fr, Rat)
• Quach ND, Kaur SP, Eggert MW et al. Paradoxical Role of Glypican-1 in Prostate Cancer Cell and Tumor Growth Sci Rep Aug 7 2019 [PMID: 31391540] (IHC, Human, Mouse)
• Zhou JY, Zhang SW, Lin HL et al. Hydrolyzed wheat gluten alleviates deoxynivalenol-induced intestinal injury by promoting intestinal stem cell proliferation and differentiation via upregulation of Wnt/beta-catenin signaling in mice Food Chem Toxicol. Jun 13 2019 [PMID: 31202940] (WB)
• Capurro MI, Greenfield LK, Prashar A et al. VANGL2 regulates luminal epithelial organization and cell turnover in the mammary gland Sci Rep May 8 2019 [PMID: 31068622] (IHC, Mouse)
• Gao, B;Deng, R;Chai, Y;Chen, H;Hu, B;Wang, X;Zhu, S;Cao, Y;Ni, S;Wan, M;Yang, L;Luo, Z;Cao, X; Macrophage-lineage TRAP+ cells recruit periosteum-derived cells for periosteal osteogenesis and regeneration J. Clin. Invest. Apr 4 2019 [PMID: 30946695] (ICC/IF, Mouse)
• Zhou, JY;Huang, DG;Qin, YC;Li, XG;Gao, CQ;Yan, HC;Wang, XQ; mTORC1 signaling activation increases intestinal stem cell activity and promotes epithelial cell proliferation J. Cell. Physiol. Apr 1 2019 [PMID: 30937902] (WB, Porcine)
• Bielli, A;Scioli, MG;D'Amico, F;Tarquini, C;Agostinelli, S;Costanza, G;Doldo, E;Campione, E;Passeri, D;Coniglione, F;Orlandi, A; Cellular retinoic acid binding protein-II expression and its potential role in skin aging Aging (Albany NY) Mar 18 2019 [PMID: 30888968] (IHC-P, Mouse)
• Ibrahim ML, Klement JD, Lu C et al. Myeloid-Derived Suppressor Cells Produce IL-10 to Elicit DNMT3b-Dependent IRF8 Silencing to Promote Colitis-Associated Colon Tumorigenesis Cell Rep Dec 11 2018 [PMID: 30540937] (IHC-P, Human)
• Jensen T, Wanczyk H, Sharma I et al. Polyurethane Scaffolds Seeded with Autologous Cells Can Regenerate Long Esophageal Gaps: an Esophageal Atresia Treatment Model Journal of Pediatric Surgery Oct 1 2018 [PMID: 30429066] (ICC/IF)
• Baek H, Kim S, Choi E et al. Inhibition of Estrogen Signaling Reduces the Incidence of BRCA1-associated Mammary Tumor Formation. Int. J. Biol. Sci. Oct 25 2018 [PMID: 30416390] (WB, Mouse)
• D'Andrea P, Sciancalepore M, Veltruska k et al. Epidermal Growth Factor- Based adhesion substrates elicit myoblast scattering, proliferation, differentiation and promote satellite cell myogenic activation. Biochim Biophys Acta Mol Cell Res. Oct 18 2018 [PMID: 30343052] (ICC/IF, Mouse)
• Hou Q, Liu L, Dong Y et al. Effects of Thymoquinone on radiation enteritis in mice Sci Rep Oct 11 2018 [PMID: 30310156] (IHC, Mouse)
• Li L, Guturi KKN, Gautreau B et al. Ubiquitin ligase RNF8 suppresses Notch signaling to regulate mammary development and tumorigenesis. J. Clin. Invest. Oct 1 2018 [PMID: 30222135] (IHC-P, Mouse)
• Dower CM, Bhat N, Gebru MT et al. Targeted inhibition of ULK1 promotes apoptosis and suppresses tumor growth and metastasis in neuroblastoma. Mol. Cancer Ther. Aug 30 2018 [PMID: 30166400] (IHC-P, Mouse)
• Schmitt M, Schewe M, Sacchetti A et al. Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling Cell Rep Aug 28 2018 [PMID: 30157426] (IHC-P, ICC/IF, Mouse)
• Kamdar K, Johnson AMF, Chac D et al. Innate Recognition of the Microbiota by TLR1 Promotes Epithelial Homeostasis and Prevents Chronic Inflammation J. Immunol. May 23 2018 [PMID: 29794015] (IHC)
• Tamosiuniene R, Manouvakhova O, Mesange P et al. Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension. Circ Res 2018 Jun 8 [PMID: 29545367]
• Harris AL, Lee SE, Dawson LK et al. Targeting the cyclin dependent kinase and retinoblastoma axis overcomes standard of care resistance in BRAF V600E -mutant melanoma. Oncotarget. Feb 16 2018 [PMID: 29541385] (IHC-P)
• Li Q, Alsaidan OA, Ma Y et al. Pharmacologically targeting the myristoylation of the scaffold protein FRS2A inhibits FGF/FGFR-mediated oncogenic signaling and tumor progression. J. Biol. Chem. Mar 14 2018 [PMID: 29540482] (IHC-P)
• Li YW, Zhang C, Sheng QJ et al. Mesenchymal stem cells rescue acute hepatic failure by polarizing M2 macrophages. World J. Gastroenterol. 2017 Dec 07 [PMID: 29259373] (IHC-P, Rat)
• Chen W, Pilling D, Gomer RH. C-reactive protein (CRP) but not the related pentraxins serum amyloid P and PTX3 inhibits the proliferation and induces apoptosis of the leukemia cell line Mono Mac 6. BMC Immunol. 2017 Dec 04 [PMID: 29202702] (ICC/IF, Human)
• Harrell JC, Shroka TM, Jacobsen BM. Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer Oncogenesis. 2017 Nov 27 [PMID: 29176653] (IHC, Human)
• Wang K, Jin Q, Ruan D et al. Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing Genome Res. Dec 1 2017 [PMID: 29146772] (IHC-P, Porcine)
• Li C, Chai Y, Wang L et al. Programmed cell senescence in skeleton during late puberty Nat Commun. 2017 Nov 03 [PMID: 29101351] (ICC/IF, Mouse)
• Park SR, Kim JW, Jun HS et al. Stem Cell Secretome and Its Effect on Cellular Mechanisms Relevant to Wound Healing. Mol Ther 2018 Feb 7 [PMID: 29066165]
• Amarnani D, Machuca-Parra AI, Wong LL et al. Effect of Methotrexate on an In Vitro Patient-Derived Model of Proliferative Vitreoretinopathy Invest. Ophthalmol. Vis. Sci. 2017 Aug 01 [PMID: 28777835] (ICC/IF, Human)
• Tan Q, Joshua AM, Wang M et al. Up-regulation of autophagy is a mechanism of resistance to chemotherapy and can be inhibited by pantoprazole to increase drug sensitivity. Cancer Chemother. Pharmacol. May 1 2017 [PMID: 28378028] (Human)
• Jayaraman S, Doucet M, Kominsky SL. Down-regulation of CITED2 attenuates breast tumor growth, vessel formation and TGF-B-induced expression of VEGFA. Oncotarget. Jan 24 2017 [PMID: 28008154] (Human)
• Peng Y, Chen FF, Ge J et al. miR-429 Inhibits Differentiation and Promotes Proliferation in Porcine Preadipocytes. Int J Mol Sci. Dec 7 2016 [PMID: 27941616] (ICC/IF, Porcine)
• Maeda A, BSc, Chen Y et al. In Vivo Imaging Reveals Significant Tumor Vascular Dysfunction and Increased Tumor HIF-1alpha Expression Induced by High Single-Dose Irradiation in a Pancreatic Tumor Model Int J Radiat Oncol Biol Phys Sep 14 2016 [PMID: 27816364] (IHC, Mouse)
• Zimmermann N, Thormann V, Hu B et al. Human isotype-dependent inhibitory antibody responses against Mycobacterium tuberculosis. EMBO Mol Med Nov 2 2016 [PMID: 27729388] (IHC-P, ICC/IF, Human)
• Xiong J, Zhou MF, Wang YD et al. Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice Sci Rep Sep 23 2016 [PMID: 27659202] (IHC, Human)
• Huang SW, Lien JC, Kuo SC, Huang TF. DDA suppresses angiogenesis and tumor growth of colorectal cancer in vivo through decreasing VEGFR2 signaling. Oncotarget. Sep 27 2016 [PMID: 27517319] (IHC, Mouse)
• Rock K, Joosse SA, Muller J et al. Chronic UVB-irradiation actuates perpetuated dermal matrix remodeling in female mice: Protective role of estrogen. Sci Rep. Jul 27 2016 [PMID: 27460287] (IHC, Mouse)
• Lohse I, Rasowski J, Cao P et al. Targeting hypoxic microenvironment of pancreatic xenografts with the hypoxia-activated prodrug TH-302. Oncotarget. Jun 7 2016 [PMID: 27248663] (Human)
• Fukuda Tatsuya, Fukuchi Tomokazu, Yagi Shinomi et al. Immunohistochemical analyses of cell cycle progression and gene expression of biliary epithelial cells during liver regeneRation after partial hepatectomy of the mouse. Experimental Animals 2016 [PMID: 26633692] (IHC, Mouse)
• de la Puente P, Muz B, Gilson RC et al. 3D tissue-engineered bone marrow as a novel model to study pathophysiology and drug resistance in multiple myeloma Biomaterials 2015 Sep 12 [PMID: 26402156] (IHC-P, Human)
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Nakagawa Y, Muneta T, Kondo S et al. Synovial mesenchymal stem cells promote healing after meniscal repair in microminipigs Osteoarthr. Cartil. 2015 Feb 13 [PMID: 25683149] (IHC-P, Porcine)
• Kawanishi Y, Nakasa T, Shoji T et al. Intra-articular injection of synthetic microRNA-210 accelerates avascular meniscal healing in rat medial meniscal injured model. Arthritis Res. Ther. 2014 Nov 28 [PMID: 25430980] (IHC-P, Rat)
  
  Details:
  This Ki67/MKI67 antibody was used (dilution 1:100) for IHC-P in Spraque Dawley rats subjected to intra-articular injection of double-stranded miR-210 (for control group using control dsRNA) with atelocollagen administered immediately after injury (injured meniscal healing in a rat model) - 4%PFA fixation, 4 um sections, and blocked with horse serum (Figure 4).
• Uegaki T, Taniguchi F, Nakamura K et al. Inhibitor of apoptosis proteins (IAPs) may be effective therapeutic targets for treating endometriosis Hum. Reprod. 2014 Nov 05 [PMID: 25376458] (IHC-P, Mouse)
• Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014 Oct 15 [PMID: 25326682] (IHC-P, Human)
• Xie H, Cui Z, Wang L et al. PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis. Nat. Med. 2014 Oct 05 [PMID: 25282358] (IHC, Mouse)
• Smith Corey L, Matheson Timothy D, Trombly Daniel J et al. A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli. Mol Biol Cell. 2014 Sep 15 [PMID: 25057015] (Human)
• Takano N, Sarfraz Y, Gilkes DM et al. Decreased Expression of Cystathionine b-Synthase Promotes Glioma Tumorigenesis. Mol. Cancer Res. 2014 Jul 03 [PMID: 24994751] (IHC-P, Human)
  
  Details:
  Ki67 antibody used for IHC-P on tumor xenografts derived from injection of parental U87-MG cells and shCBS#07 subclone (Fig 1E, Fig 3D).
• Sherman-Baust CA, Kuhn E, Valle BL et al. A genetically engineered ovarian cancer mouse model based on fallopian tube transformation mimics human high-grade serous carcinoma development. J. Pathol. 3/20/2014 [PMID: 24652535] (IHC-P, Mouse)
• Sano M, Sasaki T, Hirakawa S et al. Lymphangiogenesis and angiogenesis in abdominal aortic aneurysm. PLoS ONE 3/21/2014 [PMID: 24651519] (IHC-P, Human)
• Kwon S, Kim MS, Lee ES, Park JK. A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells. Integr Biol (Camb) 2014 Mar 5 [PMID: 24599518] (IHC-P, ICC/IF, Human)
• Mertens Marianne E, Hermann Alina, Buhren Anne et al. Iron Oxide-labeled Collagen Scaffolds for Non-invasive MR Imaging in Tissue Engineering. Adv Funct Mater. 2014 Feb 12 [PMID: 24569840]
• Gou HF, Huang J, Shi HS, Wang YS. Chemo-immunotherapy with oxaliplatin and interleukin-7 inhibits colon cancer metastasis in mice. PLoS ONE 2014 Jan 27 [PMID: 24465710] (IHC-P, Mouse)
• Saggar JK, Tannock IF. Activity of the hypoxia-activated pro-drug TH-302 in hypoxic and perivascular regions of solid tumors and its potential to enhance therapeutic effects of chemotherapy. Int J Cancer. 2013 Nov 8 [PMID: 24338277] (IHC-Fr, ICC/IF, Human)
• Xiang L, Gilkes DM, Chaturvedi P, Semenza GL et al. Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer. J Mol Med (Berl). 2013 Nov 20 [PMID: 24248265]
• Niedworok C, Rock K, Kretschmer I et al. Inhibitory Role of the Small Leucine-Rich Proteoglycan Biglycan in Bladder Cancer. PLoS One. 2013 Nov 6 [PMID: 24223213] (IHC, Human)
• Liu Y, Sanchez-Tillo E, Lu X et al. Rb1 family mutation is sufficient for sarcoma initiation. Nat Commun. 2013 Oct 23 [PMID: 24150016] (ICC/IF, Mouse)
• Spiegelberg L, Braks J, Djasim U et al. Effects of hyperbaric oxygen therapy on the viability of irradiated soft head and neck tissues in mice. Oral Dis 2013 Jul 8 [PMID: 23865954] (IHC-P, Mouse)
• Biddlestone-Thorpe L, Sajjad M, Rosenberg E et al. ATM Kinase Inhibition Preferentially Sensitizes p53-Mutant Glioma to Ionizing Radiation. Clin Cancer Res 2013 Jun 15 [PMID: 23620409] (IHC-Fr, ICC/IF, Human)
• Kirby ED, Muroy SE, Sun WG et al. Acute stress enhances adult rat hippocampal neurogenesis and activation of newborn neurons via secreted astrocytic FGF2. Elife 2013 Apr 16 [PMID: 23599891] (IHC-Fr, ICC/IF, Rat)
• Mildner M, Hacker S, Haider T et al. Secretome of Peripheral Blood Mononuclear Cells Enhances Wound Healing PLoS One 2013 [PMID: 23533667] (IHC-P, ICC/IF, Human)
• Imamura F, Greer CA. Pax6 regulates Tbr1 and Tbr2 expressions in olfactory bulb mitral cells. Mol Cell Neurosci 2013 Jan 22 [PMID: 23353076] (IHC-Fr, ICC/IF, Mouse)
• Saggar JK, Fung AS, Patel KJ, Tannock IFet al. Use of molecular biomarkers to quantify the spatial distribution of effects of anticancer drugs in solid tumors. Mol Cancer Ther 2013 Apr [PMID: 23348047] (IHC, ICC/IF, Human)
• Tao YM, Huang JL, Zeng S et al. BTB/POZ domain-containing protein 7: Epithelial-mesenchymal transition promoter and prognostic biomarker of hepatocellular carcinoma. Hepatology 2013 Jan 16 [PMID: 23325674] (IHC-P, Human, Mouse)
• Zhang Y, Srinivasan B, Xing C, Lu J. A New Chalcone Derivative (E)-3-(4-Methoxyphenyl)-2-methyl-1-(3,4,5-trimethoxyphenyl)prop-2-en-1-one Suppresses Prostate Cancer Involving p53-mediated Cell Cycle Arrests and Apoptosis Anticancer Res 2012 Sep [PMID: 22993307]
• Lee NJ, Choi DY, Song JK et al. Deficiency of C-C chemokine receptor 5 suppresses tumor development via inactivation of NF-{kgreen}B and inhibition of monocyte chemoattractant protein-1 in urethane-induced lung tumor model Carcinogenesis 2012 Oct 5 [PMID: 22907530] (IHC, Mouse)
• Rauhavirta T, Oittinen M, Kivisto R et al. Are Transglutaminase 2 Inhibitors Able to Reduce Gliadin-Induced Toxicity Related to Celiac Disease? A Proof-of-Concept Study J Clin Immunol 2012 Aug 10 [PMID: 22878839] (IHC-Fr, Human)
• Wu X, Zhang Q, Wang X et al. Survivin Is Required for Beta-Cell Mass Expansion in the Pancreatic Duct-Ligated Mouse Model plos One 2012 [PMID: 22870272] (IHC, Mouse)
• Chen F, Loturco J. A method for stable transgenesis of radial glia lineage in rat neocortex by piggyBac mediated transposition. J Neurosci Methods. 2012 Apr 11. [PMID: 22521325] (IHC, Rat)
• Rock K, Meusch M, Fuchs N et al. Estradiol Protects Dermal Hyaluronan/Versican Matrix during Photoaging by Release of Epidermal Growth Factor from Keratinocytes J Biol Chem 2012 Jun 8 [PMID: 22493503] (IHC-Fr, Mouse)
• McCaskey SJ, Rondini EA, Langohr IM, Fenton JI. Differential effects of energy balance on experimentally-induced colitis World J Gastroenterol 2012 Feb 21 [PMID: 22363133] (IHC, Mouse)
• Gaur S, Chen L, Yen T et al. Preclinical study of the cyclodextrin-polymer conjugate of camptothecin CRLX101 for the treatment of gastric cancer Nanomedicine 2011 Oct 25 [PMID: 22033079] (IHC, Mouse)
• Zhao Z. Endoplasmic reticulum stress in maternal diabetes-induced cardiac malformations during critical cardiogenesis period. Birth Defects Res B Dev Reprod Toxicol. 2011 Sep 15. [PMID: 21922638] (Mouse)
• Kim SS, Cao L, Baek HJ et al. Impaired skin and mammary gland development and increased gamma-irradiation-induced tumorigenesis in mice carrying a mutation of S1152-ATM phosphorylation site in Brca1. Cancer Res;69(24):9291-300. 2009 Dec 15. [PMID: 19996295]
• Tharayil V S, Wouters M M, Stanich J E et al. Lack of serotonin 5-HT2B receptor alters prolifeRation and network volume of interstitial cells of Cajal in vivo. Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society 2010 [PMID: 19941613] (IHC, Mouse)
• Salo S, Boutaud A, Hansen AJ et al. Antibodies blocking adhesion and matrix binding domains of laminin-332 inhibit tumor growth and metastasis in vivo Int J Cancer. 2009 Oct 15 [PMID: 19582877] (IHC-P, Human)
• Elder SH, Cooley AJ Jr, Borazjani A et al. Production of hyaline-like cartilage by bone marrow mesenchymal stem cells in a self-assembly model. Tissue Eng Part A 2009 Oct [PMID: 19335060] (IHC)
• ampa VM, Gutierrez-Lanza R, Cerignoli F et al. Notch activates cell cycle reentry progression in quiescent cardiomyocytes. J Cell Biol;183(1):129-141. 2008 [PMID: 18838555]
• Maeda N, Kokai Y, Ohtani S et al. Anti-tumor effect of orally administered spinach glycolipid fraction on implanted cancer cells, colon-26, in mice. Lipids 2008 Aug [PMID: 18594894] (IHC-P, Mouse)
• Olofsson, P S, L A Soderstrom et al. CD137 Is Expressed in Human Atherosclerosis Promotes Development of Plaque Inflammation in Hypercholesterolemic Mice. Circulation: CIRCULATIONAHA107.699173. 2008 [PMID: 18285570]
• Nam, S Y, J S Kim et al. DA-6034, a Derivative of Flavonoid, Prevents Ameliorates Dextran Sulfate Sodium-Induced Colitis Inhibits Colon Carcinogenesis. Experimental Biology Medicine 233: 180-191. 2008 [PMID: 18222973]
• Dai G, Freudenberger T, Zipper P et al. Chronic Ultraviolet B Irradiation Causes Loss of Hyaluronic Acid from Mouse Dermis Because of Down-Regulation of Hyaluronic Acid Synthases. Am J Pathol, 2007;171(5):1451-61. 2007 November 1. [PMID: 17982124] (IHC)
• Kojima, Kyoko et al. Inactivation of Smad4 Accelerates Kras G12D-Mediated Pancreatic Neoplasia. Cancer Res 67: 8121-8130. 2007 [PMID: 17804724]
• Ip, M et al. The t10,c12 isomer of conjugated linoleic acid stimulates mammary tumorigenesis in transgenic mice over-expressing erbB2 in the mammary epithelim. Carcinogenesis; 28: 1269 - 1276. 2007 June. [PMID: 17259656]
• Kelly, P et al. THe G12 family of heterotrimeric G proteins promotes breast cancer invasion metastasis. PNAS 103:8173-8178. 2006 [PMID: 16705036]
• Micci, MA et al. Caspase inhibition increases survival of neural stem cells in the gastrointestinal tract. Neurogastroenterol Motil 17: 557-64. 2005 [PMID: 16078945]
• Zecchini, V et al. Notch signaling regulates the differentiation of post-mitotic intestinal epithelial cells. Genes Dev 19: 1686-91. 2005 [PMID: 16024658]
• Knott, AW et al. Smooth muscle overexpression of IGF-I induces a novel adaptive response to small bowel resection. Am J Physiol Gastrointest Liver Physiol 287: G562-70. 2004 [PMID: 15142831]
• Gerdes, J et al. Immunobiochemical molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67. Am J Pathol 138: 867-73. 1991 [PMID: 2012175]
• Gao B, Yin J, Xu X, et al. Leptin receptor-expressing cells represent a distinct subpopulation of notochord-derived cells and are essential for disc homoeostasis Journal of Orthopaedic Translation Dec 1 2019 (IHC, Human)
• Ge, R et al. Inhibition of Growth Metastasis of Mouse Mammary Carcinoma by Selective Inhibitor of Transforming Growth Factor-{beta} Type I Receptor Kinase In vivo. Clin, Canc Res 12: 315-4330. 2006 [PMID: 16857807] (IHC-P, Mouse)

Reviews for Ki67/MKI67 Antibody (NB500-170) (6)

Average Rating: 4.3

(Based on 6 reviews)

We have 6 reviews tested in 2 species: Human, Mouse.

reviewed by: Lang Zhao IHC-P Mouse 03/17/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse aortic root
• Species: Mouse
• Lot: CGVS0116042

reviewed by: Alexandra Ambrico ICC/IF Mouse 10/11/2016

Summary

• Application: Immunocytochemistry/Immunofluorescence
• Sample Tested: mouse prostate
• Species: Mouse

reviewed by: Natalie Erdmann ICC Human 08/24/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: Multiple myeloma cells lines LP-1 and JJN3 cytospins
• Species: Human
• Lot: G-4

Blocking

• Blocking Details: 10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: 1/100 1ary antibody diluted in 10% donkey serum/PBS 3 hours at room temperature

Secondary Antibody

• Secondary Description: Donkey F(ab")2 anti-rabbit IgG (H+L) Alexa Fluor 488
• Secondary Manufacturer Cat#: Jackson 711-546-152
• Secondary Concentration: 1/1000

Details

• Detection Notes: Zeiss AxioImager, FITC filter cube for 100 ms exposure
• Fixation Details: Immersion-fixed in 2% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
• Wash Description: 3X 5 minutes PBS after each step

Comments

• Comments: Would use this reagent at 1/200 overnight at 4oC

reviewed by: Anonymous IHC-P Human 12/29/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 19582877
• Species: Human

reviewed by: Anony- mous ICC Human 06/09/2014

Summary

• Application: Immunocytochemistry
• Species: Human
• Special Applications: Confocal immunofluorescent analysis of MCF7 cells using Ki67 antibody (NB500-170, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
• File: View PDF

reviewed by: Jian Zong WB Mouse 09/30/2010

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Mouse

FAQs for Ki67/MKI67 Antibody (NB500-170). (Showing 1 - 3 of 3 FAQs).

1. Could you please let us know if you have the antibody which may have cross-reactivity to hamster tissue in IHC(paraffin or frozen)? Also, please tell us reference information using your products to hamster tissue.
   • Thank you for your patience. The only information I was able to gain is that the protein has 61% homology with the chinese hamster. Our lab only recommends use if the % homology is 85% or above so I would not recommend our Ki67 antibodies for your customer. However if they are willing to still test this they would become eligable for our Innovators Reward Program in which they would gain a 50% refund and a 50% discount on a future purchase. All you would need to do is email us at innovators@novusbio.com. Here is the link to all of our Ki67 antibodies.
2. What is the IF protocol which was used to obtain the IF image on the datasheet?
   • Our lab used our general ICC protocol to obtain the image seen on our website.
3. Is there a clone number for this Ki67 antibody?
   • This antibody is polyclonal, and thus it does not have a clone number.

Bioinformatics

• Gene Symbol: MKI67
• Entrez:
  • 4288(Human)
  • 17345(Mouse)
  • 291234(Rat)
• Uniprot:
  • P46013(Human)
  • Q91VE6(Mouse)