Ki67/MKI67 Antibody

Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB110-89717] - Staining of a cross section of mouse spleen. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89717] - Ki-67/MKI67 Antibody [NB110-89717] - Analysis of a mouse intestine cross section. The antibody was used at a dilution of 1:250. Detection: DAB staining. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NB110-89717] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Ki-67/MKI67 at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89717] - Analysis of Ki-67 in human prostate xenograft control (left) and treated (right) using anti-Ki-67 antibody. Image from verified customer review.

Flow Cytometry: Ki67/MKI67 Antibody [NB110-89717] - An intracellular stain was performed on U-937 cells with NB110-89717PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89717] - KLF4 ablation leads to abnormal proliferation and differentiation in small intestinal epithelium. Higher magnification IHC staining of highlighted frames. Small intestine from Klf4-/- mice induced by tamoxifen for different time endurances were stained by H&E and PAS, and also immunohistochemistry staining was performed with anti-Ki67, anti-Lysozyme, anti-DCAMKL-1, and anti-PCNA antibodies respectively. Bottom panel: IHC staining with ZO-1 antibody in one-month knockout intestine tissue. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0032492) licensed under a CC-BY licence.

Flow Cytometry: Ki67/MKI67 Antibody [NB110-89717] - An intracellular stain was performed on HeLa cells with Ki-67/MKI67 antibody NB110-89717PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody [NB110-89717] - Analysis of Ki-67 in paraffin embedded mouse prostate tissue using anti-Ki-67 antibody. Image from verified customer review.

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89717] - Ki67 Antibody [NB110-89717] - FFPE section of mouse Peyer's patch. Antibody: Affinity purified rabbit anti-mouse Ki-67 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH.

Immunohistochemistry: Ki67/MKI67 Antibody [NB110-89717] - Ki-67/MKI67 Antibody [NB110-89717] - Detection of Ki67 in FFPE mouse intestine using NB110-89717.

Flow Cytometry: Ki67/MKI67 Antibody [NB110-89717] - Ki-67/MKI67 Antibody [NB110-89717] - Staining of mouse bone marrow cells using NB110-89717 at a dilution of 1:100. Photo courtesy of product review by verified customer.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Ki67/MKI67 Antibody Summary

• Immunogen: The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Mouse KI67/MKI67, between aminoacids 1850-1950 (1899-1916) Uniprot# E9PVX6.
• Localization: Nuclear
• Marker: Proliferation Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MKI67
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
• Application Notes: Ki67/MKI67 antibody validated for Flow from a verified customer review. WB (PMID: 22384261), IHC (PMID: 28832561), and ICC/IF (PMID: 24779589).
• Theoretical MW: 351 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Ki67/MKI67 Knockout HeLa Cell Lysate

Reactivity Notes

Ki67/MKI67 Antibody reacted with Rat in in scientific literature (PMID: 24275061).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ki67/MKI67 Antibody

• antigen identified by monoclonal Ki-67
• Antigen Ki67
• antigen KI-67
• Ki67
• Ki-67
• KIA
• Marker Of Proliferation Ki-67
• MIB-
• MIB-1
• MKI67
• PPP1R105
• Proliferation Marker Protein Ki-67
• proliferation-related Ki-67 antigen
• Protein Phosphatase 1
• Regulatory Subunit 105
• TSG126

Background

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ki67/MKI67 Antibody (NB110-89717)(55)

Publications using NB110-89717

• Yamamoto-Fukuda T, Akiyama N, Kojima H L1CAM-ILK-YAP mechanotransduction drives proliferative activity of epithelial cells in middle ear cholesteatoma Am. J. Pathol. Apr 29 2020 [PMID: 32360569]
• Meng C, Tian G, Xu C et al. Hippo kinases MST1 and MST2 control the differentiation of the epididymal initial segment via the MEK-ERK pathway Cell Death Differ. Apr 24 2020 [PMID: 32332916]
• Lemons K, Fu Z, Ogura T, Lin W TRPM5-expressing Microvillous Cells Regulate Region-specific Cell Proliferation and Apoptosis During Chemical Exposure Neuroscience Mar 26 2020 [PMID: 32224228] (IHC, Mouse)
• Tsai Mc, Huang Cc, Wei Yc et Al. Combined Chibby and beta-Catenin Predicts Clinical Outcomes in Patients with Hepatocellular Carcinoma Int J Mol Sci Mar 17 2020 [PMID: 32192213] (ICC/IF, Human)
• Chou Pr, Lin Yn, Wu Sh et Al. Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing Int J Med Sci Feb 4 2020 [PMID: 32132871] (IHC-P, Porcine)
• Kim YJ, Tsang T, Anderson GR et al. Inhibition of BCL2 family members increases the efficacy of copper chelation in BRAFV600E-driven melanoma Cancer Res. Jan 31 2020 [PMID: 32005716] (ICC/IF, Mouse)
• Li S, Lin Q, Shao X, et al. LRIG1 is a pleiotropic androgen receptor-regulated feedback tumor suppressor in prostate cancer Nat Commun Dec 2 2019 [PMID: 31792211] (IHC-P, Mouse)
• Li H, Yu Y, Zhao Y et al Small molecule inhibitor agerafenib effectively suppresses neuroblastoma tumor growth in mouse models via inhibiting ERK MAPK signaling. Cancer Lett. 2019 Aug 10 [PMID: 31100410] (IHC-P, Mouse)
  
  Details:
  Citation using the Allophycocyanin version of this antibody.
• Gunin, AG;Golubtsova, NN;Kravtsova, OA;Subbotkin, AS;Subbotkina, NO;Filippov, FN; Number, Proliferative Activity, and Expression of Thyroid Hormone Receptors in Dermal Fibroblasts in Mice with Changed Thyroid Status Bull. Exp. Biol. Med. Apr 26 2019 [PMID: 31028589] (IHC, Mouse)
• Li, S;Song, Y;Quach, C;Guo, H;Jang, GB;Maazi, H;Zhao, S;Sands, NA;Liu, Q;In, GK;Peng, D;Yuan, W;Machida, K;Yu, M;Akbari, O;Hagiya, A;Yang, Y;Punj, V;Tang, L;Liang, C; Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance Nat Commun Apr 12 2019 [PMID: 30979895] (IHC, Human)
• Amano H, Chaudhury A, Rodriguez-Aguayo C et al. Telomere Dysfunction Induces Sirtuin Repression that Drives Telomere-Dependent Disease Cell Metab. Mar 20 2019 [PMID: 30930169] (IHC, Mouse)
• Xu J, Song J, Yang X et al. ProNGF siRNA inhibits cell proliferation and invasion of pancreatic cancer cells and promotes anoikis Biomedicine & Pharmacotherapy Mar 1 2019 [PMID: 30841420] (ICC/IF, Human)
• Naito H, Iba T, Wakabayashi T et al. TAK1 Prevents Endothelial Apoptosis and Maintains Vascular Integrity. Dev. Cell Jan 28 2019 [PMID: 30639056] (ICC/IF, Mouse)
• Gunin, A;Golubtsova, N;Subbotkina, N;Subbotkin, A; The Influence of Metformin on Age-Related Changes in the Number and Proliferation of Dermal Fibroblasts in Mice Adv Gerontol Jan 1 2019 [PMID: 30607913] (IHC, Mouse)
• Stojiljkovic M, Ain Q, Bondeva T et al. Phenotypic and functional differences between senescent and aged murine microglia. Neurobiology of Aging. Oct 1 2018 [PMID: 30439594] (ICC/IF, Mouse)
• Kumari A, Yokota Y, Li L et al. Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib. Sci Rep. Nov 1 2018 [PMID: 30385780] (ICC/IF, Rat)
• Gopalakrishnan k, Teitelbaum SL, Wetmur J et al. Histology and Transcriptome Profiles of the Mammary Gland across Critical Windows of Development in Sprague Dawley Rats. J Mammary Gland Biol Neoplasia. Jun 28 2018 [PMID: 29956080] (IHC, Rat)
• Anurag M, Punturi N, Hoog J et al. Comprehensive profiling of DNA repair defects in breast cancer identifies a novel class of endocrine therapy resistance drivers Clin. Cancer Res. May 23 2018 [PMID: 29793947] (ICC/IF)
• Duteil D, Tourrette Y, Eberlin A et al. The histone acetyltransferase inhibitor Nir regulates epidermis development. Development. Mar 21 2018 [PMID: 29490983] (Mouse)
• Tachikawa S, Shimizu M, Maruyama K, Ohnuma K. Thalidomide induces apoptosis during early mesodermal differentiation of human induced pluripotent stem cells. In Vitro Cell Dev Biol Anim 2018 Mar [PMID: 29435726] (Human)
• Kumari P, Saha I, Narayanan A et al. Essential role of HCMV deubiquitinase in promoting oncogenesis by targeting anti-viral innate immune signaling pathways. Cell Death Dis. 2017 Oct 05 [PMID: 28981114] (Flow)
• Yamakami Y, Kohashi K, Oyama K et al. LGR4 is required for sequential molar development Biochem Biophys Rep. 2016 Aug 19 [PMID: 28955954] (IHC-P, Mouse)
• Pavlidou T, Rosina M, Fuoco C et al. Regulation of myoblast differentiation by metabolic perturbations induced by metformin PLoS ONE 2017 Aug 31 [PMID: 28859084] (ICC/IF, Mouse)
• Fishbein I, Guerrero DT, Alferiev IS, Foster JB. Stent-based delivery of adeno-associated viral vectors with sustained vascular transduction and iNOS-mediated inhibition of in-stent restenosis. Gene Ther. 2017 Nov 01 [PMID: 28832561] (IHC, Rat)
• Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 2017 Jan [PMID: 28190929]
• Kalliokoski S, Piqueras VO, Frias R et al. Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice. Amino Acids. Aug 9 2016 [PMID: 27503559] (IHC, Mouse)
• Liang X, Luo M, Wei XW et al. A folate receptor-targeted lipoplex delivering interleukin-15 gene for colon cancer immunotherapy. Oncotarget. Jul 11 2016 [PMID: 27438147] (IHC)
• Yancu D, Blouin MJ, Birman E et al. Insulin-like growth factor-3 knockout mice are resistant to experimental dextran sulfate sodium colitis. J. Gastroenterol. Hepatol. Jun 2 2016 [PMID: 27253188] (IHC)
• Kai T, Tsukamoto Y, Hijiya N et al. Kidney-specific knockout of Sav1 in the mouse promotes hyper-proliferation of renal tubular epithelium through suppression of the Hippo pathway J. Pathol. Feb 24 2016 [PMID: 26913567] (IHC-P, Mouse)
• Chen NC, Chyau CC, Lee YJ et al. Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells Sci Rep Feb 3 2016 [PMID: 26838546] (IHC-P, Human)
• Velarde MC, Demaria M, Melov S, Campisi J. Pleiotropic age-dependent effects of mitochondrial dysfunction on epidermal stem cells. Proc. Natl. Acad. Sci. U.S.A. 2015 Aug 18 [PMID: 26240345] (IHC-P, Mouse)
• Nowak DG, Cho H, Herzka T et al. Myc drives Pten/ p53-deficient proliferation and metastasis due to Il6-secretion and Akt-suppression via Phlpp2 Cancer Discov 2015 Mar 31 [PMID: 25829425] (IHC-P, Mouse)
• Aggarwal A, Hobaus J, Tennakoon S et al. Active vitamin D potentiates the anti-neoplastic effects of calcium in the colon: A cross talk through the calcium-sensing receptor. J. Steroid Biochem. Mol. Biol. 2015 Mar 07 [PMID: 25758239] (IHC-P, Mouse)
• Franz H, Greschik H, Willmann D et al. The histone code reader SPIN1 controls RET signaling in liposarcoma. Oncotarget 2015 Mar 10 [PMID: 25749382] (ICC/IF)
• Lindley LE, Curtis KM, Sanchez-Mejias A et al. The WNT-controlled transcriptional regulator LBH is required for mammary stem cell expansion and maintenance of the basal lineage Development. 2015 Mar 01 [PMID: 25655704] (IHC-P, Mouse)
  
  Details:
  Ki-67/MKI67 antibody used at 1:300 dilution for IHC staining of 5?um sections from 4% paraformaldehyde fixed paraffin-embedded abdominal mouse mammary gland tissues of K14-Cre; Lbh+/+ wild-type (WT) and K14-Cre;LbhloxP/loxP mice. Fig 2E documents the Ki67 immunostaining/expression levels in tested tissues demonstrating reduced cell proliferation of basal and luminal cells in terminal end buds/TEBs of K14-Cre;LbhloxP/loxP mice.
• Zhou Y, Rychahou P, Wang Q et al. TSC2/mTORC1 signaling controls Paneth and goblet cell differentiation in the intestinal epithelium Cell Death Dis. 2015 Feb 06 [PMID: 25654764] (IHC-P, Mouse)
• Rachidi S, Sun S, Wu Bx et al. Endoplasmic reticulum heat shock protein gp96 maintains liver homeostasis and promotes hepatocellular carcinogenesis. J. Hepatol. 2014 Nov 22 [PMID: 25463537]
• Mathes Al, Rice L, Affandi Aj et al. CpGB DnA activates dermal macrophages and specifically recruits inflammatory monocytes into the skin. Exp. Dermatol. 2014 Nov 25 [PMID: 25425469]
• Laskov I, Drudi L, Beauchamp MC et al. Anti-diabetic doses of metformin decrease proliferation markers in tumors of patients with endometrial cancer Gynecol. Oncol. 2014 Jun 24 [PMID: 24972190] (IHC-P, Human)
  
  Details:
  Ki67 antibody used for IHC-P in human endometrial cancer - diagnostic endometrial biopsy/before metformin and definitive surgery/after metformin treatment. FFPE sections stained on Discovery XT Autostainer with heat-induced epitope retrieval (Cell Conditioning solution CC1 pH 8.0 at 95 C for 60 min), primary diluted at 1:100, 32 min incubation at 37 C followed by the detection using Omnimap anti-Rabbit HRP and ChromoMap-DAB reagents (See full text for detailed protocol and staining images- Fig. 2A-B, Fig. 4 A-B).
• Fong EL, Martinez M, Yang J et al. Hydrogel-Based 3D Model of Patient-Derived Prostate Xenograft Tumors Suitable for Drug Screening. Mol. Pharm. 2014 Apr 29 [PMID: 24779589] (ICC/IF, Human)
• Beristain AG, Molyneux SD, Joshi PA et al. PKA signaling drives mammary tumorigenesis through Src. Oncogene 3/24/2014 [PMID: 24662820] (IHC-P, Mouse)
• Kaenel P, Hahnewald S, Wotzkow C et al. Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization. Dev. Growth Differ. 3/18/2014 [PMID: 24635767] (IHC-P, Mouse)
• Yakimchuk Konstantin, Hasni Mohammad Sharif, Guan Jiyu et al. Inhibition of lymphoma vascularization and dissemination by estrogen receptor beta agonists. Blood. 2014 Mar 27 [PMID: 24470591]
• Jones Huw B, Reens Jaimini, Brocklehurst Simon R et al. Islets of Langerhans from prohormone convertase-2 knockout mice show alpha-cell hyperplasia and tumorigenesis with elevated alpha-cell neogenesis. Int J Exp Pathol. 2014 Feb 01 [PMID: 24456331] (Mouse)
• Pusic AD, Pusic KM, Clayton BL et al. IFNgamma-stimulated dendritic cell exosomes as a potential therapeutic for remyelination. J Neuroimmunol. 2013 Nov 9 [PMID: 24275061] (IHC, ICC/IF, Rat)
• Xiang L, Gilkes DM, Chaturvedi P et al. Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer. J Mol Med (Berl). 2013 Nov 20 [PMID: 24248265] (IHC-P, Mouse)
• He ZY, Wei XW, Luo M et al. Folate-linked lipoplexes for short hairpin RNA targeting claudin-3 delivery in ovarian cancer xenografts. J Control Release. 2013 Oct 18 [PMID: 24144916] (WB, Human)
• Tang Y, Cheng Y, Martinka M et al. Prognostic significance of KAI1/CD82 in Human melanoma and its role in cell migration and invasion through the regulation of ING4. Carcinogenesis. 2013 Nov 16 [PMID: 24130172] (WB, IHC, Human)
• Saha MN, Jiang H, Yang Y et al. PRIMA-1Met/APR-246 Displays High Antitumor Activity in Multiple Myeloma By Induction of p73 and Noxa. Mol Cancer Ther. 2013 Nov [PMID: 24030633] (IHC, Human)
• Steven A, Leisz S, Massa C et al. HER-2/neu Mediates Oncogenic Transformation via Altered CREB Expression and Function. Mol Cancer Res. 2013 Nov [PMID: 24025972] (IHC-P, Mouse)
• Goo JS, Kim YB, Shim SB et al. Nicastrin Overexpression in Transgenic Mice Induces Aberrant Behavior and APP Processing. Mol Neurobiol 2013 Apr 18 [PMID: 23595812] (IHC-P, Mouse)
• Jakate K, Azimi F, Ali RH et al. Endometrial sarcomas: an immunohistochemical and JAZF1 re-arrangement study in low-grade and undifferentiated tumors Mod Pathol 2012 Aug 24 [PMID: 22918161] (IHC, Human)
• Yu T, Chen X, Zhang W, Li J, Xu R, Wang TC, Ai W, Liu C. Kruppel-like Factor 4 Regulates Intestinal Epithelial Cell Morphology and Polarity. PLoS One;7(2):e32492. 2012 [PMID: 22384261]
• Kiyota T, Ingraham KL, Swan RJ et al. AAV serotype 2/1-mediated gene delivery of anti-inflammatory interleukin-10 enhances neurogenesis and cognitive function in APP+PS1 mice. Gene Ther. 2011 Sep 15. [PMID: 21918553] (ICC/IF, Mouse)
• Baek HJ, Pishvaian MJ, Tang Y, Kim TH, Yang S, Zouhairi ME, Mendelson J, Shetty K, Kallakury B, Berry DL, Shin KH, Mishra B, Reddy EP, Kim SS, Mishra L. Transforming growth factor-? adaptor, ?2-spectrin, modulates cyclin dependent kinase 4 to reduce development of hepatocellular cancer. Hepatology;53(5):1676-84. 2011 May. [PMID: 21520178] (IHC, Mouse)

Reviews for Ki67/MKI67 Antibody (NB110-89717) (7)

Average Rating: 4.6

(Based on 7 reviews)

We have 7 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Anonymous Flow Human 06/16/2017

Summary

• Application: Flow Cytometry
• Sample Tested: Blood mononuclear cells (PBMCs)
• Species: Human

reviewed by: Anonymous IF Rat 01/06/2016

Summary

• Application: Immunofluorescence
• Species: Rat
• Lot: 030209
• File: View PDF

reviewed by: Alexandra Ambrico IHC-P Mouse 06/16/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse prostate
• Species: Mouse

reviewed by: Anonymous Mouse 04/28/2015

Summary

• Species: Mouse
• File: View PDF

reviewed by: Ramakumar Tummala IHC-P Human 01/08/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Xenografts
• Species: Human
• Lot: 050806-1

reviewed by: Anonymous IHC-P Mouse 12/08/2011

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Liver Tissue
• Species: Mouse

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: overnight

Secondary Antibody

• Secondary Description: Secondary Ab: Rat HRP-Polymer (DAKO)

Details

• Detection Notes: Detection Method: DAB (HRP)

reviewed by: Huiyuan Zhang Flow Mouse 07/14/2010

Summary

• Application: Flow Cytometry
• Sample Tested: mouse bone marrow cells
• Species: Mouse
• Comments: I tried your antibody for Flow staining. I used the common intracellular staining protocol and dilution of Ki67 is 1:100.

Comments

• Comments: I tried your antibody for Flow staining. I used the common intracellular staining protocol and dilution of Ki67 is 1:100.

FAQs for Ki67/MKI67 Antibody (NB110-89717). (Showing 1 - 3 of 3 FAQs).

1. We have a customer who is using NB110-89717. She has seen the statement on one of the IHC images that says, Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH. She would like to know if you recommend Tris buffer pH 10 or if not what you do recommend for the best results. Any info you have is much appreciated.
   • Yes, the customer may use a Tris pH 10 buffer if that is what she wish to and we believe it should work fine. If she don't get the expected results we would recommend switching to a citrate buffer pH 6.0 as this is what our lab typically tests our products with.
2. About the Ki-67/MKI67 antibody NB110-89717,does it contain BSA as a carrier protein?
   • Our product NB110-89717 is provided in PBS pH 7.4 with 0.05% sodium azide as preservative. The buffer does not contain any BSA.
3. Is there a clone number for this Ki67 antibody?
   • This antibody is polyclonal, and thus it does not have a clone number.

Bioinformatics

• Gene Symbol: MKI67
• Entrez:
  • 4288(Human)
  • 17345(Mouse)
• Uniprot:
  • Q61769(Mouse)