Ki-67/MKI67 Antibody

Images

 
Western Blot: Ki67 Antibody [NBP2-19012] - Western blot analysis of A431 (A), HeLa (B), Ntera2 (C), and HEK293 (D) cell lysate using Ki67 antibody (NBP2-19012) at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Ki67 Antibody [NBP2-19012] - Ki67 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). ...read more
Immunohistochemistry-Paraffin: Ki67 Antibody [NBP2-19012] - Formalin-fixed, paraffin-embedded human breast tumor stained with Ki-67 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.
Flow Cytometry: Ki67 Antibody [NBP2-19012] - Expression in actively growing Jurkat cells: Cells were stained with 0.2 ug of Ki-67 antibody (red) and isotype control (green) and positively stained population was ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Ki-67/MKI67 Antibody Summary

Immunogen
A portion of amino acids 1200-1250 from human Ki-67was used as the immunogen for this antibody.
Localization
Nuclear
Marker
Proliferation Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MKI67
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 0.2 ug/10^6 cells
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Ki-67 appears to be limited to the activity phases of the cell-cycle
Publications
Read Publications using
NBP2-19012 in the following applications:

  • IHC
    2 publications

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in human, new world monkey, white-cheeked gibbon, rabbit, sheep, chimpanzee, and orangutan, 85% in cow, guinea pig, and rhesus monkey, 80% in mole rat, 75% in panda, horse, mouse, and rat, and 70% homologouus in dog, and chinese hamster. Mouse reactivity reported in scientific literature (PMID: 27472062).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Ki-67/MKI67 Antibody

  • antigen identified by monoclonal Ki-67
  • antigen KI-67
  • Ki67 flow cytometry
  • Ki-67 flow cytometry
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-1
  • MKI67
  • PPP1R105
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

Expression of Ki-67 appears to be limited to the activity phases of the cell-cycle (1, 2). Ki-67 is expressed in G1, G2, S and M phases but is absent in G0 or resting phase. As a proliferation marker, Ki-67 has both diagnostic and prognostic value for a metricized analysis (1-3). The Ki-67 growth fraction or Ki-67 GF has been used in a variety of cancers but especially breast cancer populations3. For example, Ki-67 criteria can be correlated to different other prognostic parameters such as tumor stage, hormone receptor status, patient age (4). Antibody to Ki-67 therefore allows analysis of positive-expressing cells in treatment regimen as well as for prognosis by IHC and Flow Cytometry as routine key platforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ki-67/MKI67 Antibody (NBP2-19012)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Discover related pathways, diseases and genes to Ki-67/MKI67 Antibody (NBP2-19012). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Ki-67/MKI67 Antibody (NBP2-19012)

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Blogs on Ki-67/MKI67.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Ki67 - an established marker for labelling proliferating cells
Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v...  Read full blog post.

Ki67 - A Crucial Cellular Proliferation Marker
The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line....  Read full blog post.

The Ki67 Antibody in Cell Marker Studies
The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is...  Read full blog post.

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Bioinformatics

Gene Symbol MKI67
Entrez