Western Blot: Ki67 Antibody [NBP2-19012] - Western blot analysis of A431 (A), HeLa (B), Ntera2 (C), and HEK293 (D) cell lysate using Ki67 antibody (NBP2-19012) at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Ki67 Antibody [NBP2-19012] - Ki67 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). ...read more
Immunohistochemistry-Paraffin: Ki67 Antibody [NBP2-19012] - Formalin-fixed, paraffin-embedded human breast tumor stained with Ki-67 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.
Flow Cytometry: Ki67 Antibody [NBP2-19012] - Expression in actively growing Jurkat cells: Cells were stained with 0.2 ug of Ki-67 antibody (red) and isotype control (green) and positively stained population was ...read more
The amino acid sequence used as immunogen for this antibody is 100% homologous in human, new world monkey, white-cheeked gibbon, rabbit, sheep, chimpanzee, and orangutan, 85% in cow, guinea pig, and rhesus monkey, 80% in mole rat, 75% in panda, horse, mouse, and rat, and 70% homologouus in dog, and chinese hamster. Mouse reactivity reported in scientific literature (PMID: 27472062).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.2 ml PBS and 0.05% BSA
0.05% Sodium Azide
Protein A purified
Alternate Names for Ki-67/MKI67 Antibody
antigen identified by monoclonal Ki-67
Ki67 flow cytometry
Ki-67 flow cytometry
Marker Of Proliferation Ki-67
proliferation-related Ki-67 antigen
Protein Phosphatase 1
Regulatory Subunit 105
Expression of Ki-67 appears to be limited to the activity phases of the cell-cycle (1, 2). Ki-67 is expressed in G1, G2, S and M phases but is absent in G0 or resting phase. As a proliferation marker, Ki-67 has both diagnostic and prognostic value for a metricized analysis (1-3). The Ki-67 growth fraction or Ki-67 GF has been used in a variety of cancers but especially breast cancer populations3. For example, Ki-67 criteria can be correlated to different other prognostic parameters such as tumor stage, hormone receptor status, patient age (4). Antibody to Ki-67 therefore allows analysis of positive-expressing cells in treatment regimen as well as for prognosis by IHC and Flow Cytometry as routine key platforms.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The Ki67 Antibody in Cell Marker Studies The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is... Read full blog post.
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