Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB110-89717] - Staining of a cross section of mouse spleen. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was ...read more
Immunohistochemistry: Ki-67/MKI67 Antibody [NB110-89717] - Ki67 Antibody [NB110-89717] - FFPE section of mouse peyer's patch. Antibody: Affinity purified rabbit anti-mouse Ki-67 used at a dilution of 1:250. Detection: ...read more
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB110-89717] - analysis of Ki-67 in human prostate xenograft control (left) and treated (right) using anti-Ki-67 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB110-89717] - analysis of Ki-67 in paraffin embedded mouse prostate tissue using anti-Ki-67 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Ki-67/MKI67 Antibody [NB110-89717] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry: Ki-67/MKI67 Antibody [NB110-89717] - Immunohistochemical analysis of mouse spleen.
Flow Cytometry: Ki-67/MKI67 Antibody [NB110-89717] - An intracellular stain was performed on HeLa cells with Ki-67/MKI67 antibody NB110-89717PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Immunohistochemistry: Ki-67/MKI67 Antibody [NB110-89717] - Analysis of a mouse intestine cross section. The antibody was used at a dilution of 1:250. Detection: DAB staining. Epitope Retrieval Buffer-High pH was ...read more
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB110-89717] - Analysis of Ki-67 in a formalin fixed paraffin embedded cross section of mouse intestine. Detection: DAB staining using Immunohistochemistry Accessory ...read more
Immunohistochemistry: Ki-67/MKI67 Antibody [NB110-89717] - Detection of Ki67 in formalin-fixed paraffin embedded mouse intestine using NB110-89717.
Flow Cytometry: Ki-67/MKI67 Antibody [NB110-89717] - An intracellular stain was performed on U-937 cells with Ki-67/MKI67 Antibody NB110-89717PE (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: Ki-67/MKI67 Antibody [NB110-89717] - Staining of mouse bone marrow cells using NB110-89717 at a dilution of 1:100. Photo courtesy of product review by verified customer.
This Ki67 antibody is useful for Flow Cytometry (customer review), Western blot (PMID 22384261), IHC and IF/ICC. Use on frozen sections reported in scientific literature.
324 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human and mouse. Rat reactivity reported in scientific literature (PMID: 24275061)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for Ki-67/MKI67 Antibody
antigen identified by monoclonal Ki-67
Marker Of Proliferation Ki-67
proliferation-related Ki-67 antigen
Protein Phosphatase 1
Regulatory Subunit 105
Originally discovered employing mouse monoclonal antibody against a nuclear antigen from Hodgkin's lymphoma-derived cell line, this non-histone protein was named Ki67 after researcher's location (Gerdes and colleagues), Ki for Kiel University in Germany and 67 referring to the clone number on the 96-well plate. It interacts with KIF15 as well as MKI67IP, and is suggested to be involved in cell cycle regulation. Ki67 is a large protein with expected molecular weight of about 395 kD and has a very complex localization pattern within the nucleus, one which changes during cell cycle progression. Its expression occurs specially during late G1, S, G2 and M phases of the cell cycle, while in cells undergoing G0 phase, Ki67 remains undetectable. Ki67 undergoes phosphorylation/dephosphorylation during mitosis, is susceptible to proteases and its structure implies that its expression is regulated by proteolytic pathways. Ki67 is associated with nucleolar DFC (dense fibrillary component) and its regulation appears to be tightly controlled (estimated half life is 60-90 min, regardless of the cell position in the cell cycle), presumably by precise synthesis and degradation systems involving proteasome, a protease complex. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Ki-67/MKI67 Antibody (NB110-89717). (Showing 1 - 2 of 2 FAQs).
We have a customer who is using NB110-89717. She has seen the statement on one of the IHC images that says, Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH. She would like to know if you recommend Tris buffer pH 10 or if not what you do recommend for the best results. Any info you have is much appreciated.
Yes, the customer may use a Tris pH 10 buffer if that is what she wish to and we believe it should work fine. If she don't get the expected results we would recommend switching to a citrate buffer pH 6.0 as this is what our lab typically tests our products with.
About the Ki-67/MKI67 antibody NB110-89717,does it contain BSA as a carrier protein?
Our product NB110-89717 is provided in PBS pH 7.4 with 0.05% sodium azide as preservative. The buffer does not contain any BSA.
The Ki67 Antibody in Cell Marker Studies The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is... Read full blog post.
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