Ki-67/MKI67 Antibody

Images

 
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB110-89717] - Staining of a cross section of mouse spleen. Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was ...read more
Immunohistochemistry: Ki-67/MKI67 Antibody [NB110-89717] - Ki67 Antibody [NB110-89717] - FFPE section of mouse peyer's patch. Antibody: Affinity purified rabbit anti-mouse Ki-67 used at a dilution of 1:250. Detection: ...read more
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB110-89717] - analysis of Ki-67 in human prostate xenograft control (left) and treated (right) using anti-Ki-67 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB110-89717] - analysis of Ki-67 in paraffin embedded mouse prostate tissue using anti-Ki-67 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Ki-67/MKI67 Antibody [NB110-89717] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry: Ki-67/MKI67 Antibody [NB110-89717] - Immunohistochemical analysis of mouse spleen.
Flow Cytometry: Ki-67/MKI67 Antibody [NB110-89717] - An intracellular stain was performed on HeLa cells with Ki-67/MKI67 antibody NB110-89717PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Immunohistochemistry: Ki-67/MKI67 Antibody [NB110-89717] - Analysis of a mouse intestine cross section. The antibody was used at a dilution of 1:250. Detection: DAB staining. Epitope Retrieval Buffer-High pH was ...read more
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB110-89717] - Analysis of Ki-67 in a formalin fixed paraffin embedded cross section of mouse intestine. Detection: DAB staining using Immunohistochemistry Accessory ...read more
Immunohistochemistry: Ki-67/MKI67 Antibody [NB110-89717] - Detection of Ki67 in formalin-fixed paraffin embedded mouse intestine using NB110-89717.
Flow Cytometry: Ki-67/MKI67 Antibody [NB110-89717] - An intracellular stain was performed on U-937 cells with Ki-67/MKI67 Antibody NB110-89717PE (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: Ki-67/MKI67 Antibody [NB110-89717] - Staining of mouse bone marrow cells using NB110-89717 at a dilution of 1:100. Photo courtesy of product review by verified customer.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Ki-67/MKI67 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the mouse Ki67 protein (within residues 1600-1700). [Swiss-Prot# Q61769]
Localization
Nuclear
Marker
Proliferation Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MKI67
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
This Ki67 antibody is useful for Flow Cytometry (customer review), Western blot (PMID 22384261), IHC and IF/ICC. Use on frozen sections reported in scientific literature.
Theoretical MW
324 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Ki-67/MKI67 Lysate (NBP2-64670)
Reviewed Applications
Read 7 Reviews rated 4.6
using
NB110-89717 in the following applications:

Publications
Read Publications using
NB110-89717 in the following applications:

  • 1 publication
  • 6 publications
  • IHC
    10 publications
  • 13 publications
  • WB
    2 publications

Reactivity Notes

Human and mouse. Rat reactivity reported in scientific literature (PMID: 24275061)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ki-67/MKI67 Antibody

  • antigen identified by monoclonal Ki-67
  • antigen KI-67
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-1
  • MKI67
  • PPP1R105
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

Originally discovered employing mouse monoclonal antibody against a nuclear antigen from Hodgkin's lymphoma-derived cell line, this non-histone protein was named Ki67 after researcher's location (Gerdes and colleagues), Ki for Kiel University in Germany and 67 referring to the clone number on the 96-well plate. It interacts with KIF15 as well as MKI67IP, and is suggested to be involved in cell cycle regulation. Ki67 is a large protein with expected molecular weight of about 395 kD and has a very complex localization pattern within the nucleus, one which changes during cell cycle progression. Its expression occurs specially during late G1, S, G2 and M phases of the cell cycle, while in cells undergoing G0 phase, Ki67 remains undetectable. Ki67 undergoes phosphorylation/dephosphorylation during mitosis, is susceptible to proteases and its structure implies that its expression is regulated by proteolytic pathways. Ki67 is associated with nucleolar DFC (dense fibrillary component) and its regulation appears to be tightly controlled (estimated half life is 60-90 min, regardless of the cell position in the cell cycle), presumably by precise synthesis and degradation systems involving proteasome, a protease complex. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ki-67/MKI67 Antibody (NB110-89717)(37)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow, ICC/IF, IHC, IHC-P, WB.


Filter By Application
Flow
(1)
ICC/IF
(6)
IHC
(10)
IHC-P
(13)
WB
(2)
All Applications
Filter By Species
Human
(7)
Mouse
(17)
Rat
(3)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NB110-89717 Applications Species
Gopalakrishnan k, Teitelbaum SL, Wetmur J et al. Histology and Transcriptome Profiles of the Mammary Gland across Critical Windows of Development in Sprague Dawley Rats. J Mammary Gland Biol Neoplasia. Jun 28 2018 [PMID: 29956080] (IHC, Rat) IHC Rat
Anurag M, Punturi N, Hoog J et al. Comprehensive profiling of DNA repair defects in breast cancer identifies a novel class of endocrine therapy resistance drivers Clin. Cancer Res. May 23 2018 [PMID: 29793947] (ICC/IF) ICC/IF
Duteil D, Tourrette Y, Eberlin A et al. The histone acetyltransferase inhibitor Nir regulates epidermis development. Development. Mar 21 2018 [PMID: 29490983] (Mouse) Mouse
Kumari P, Saha I, Narayanan A et al. Essential role of HCMV deubiquitinase in promoting oncogenesis by targeting anti-viral innate immune signaling pathways. Cell Death Dis. 2017 Oct 05 [PMID: 28981114] (Flow) Flow
Pavlidou T, Rosina M, Fuoco C et al. Regulation of myoblast differentiation by metabolic perturbations induced by metformin PLoS ONE 2017 Aug 31 [PMID: 28859084] (ICC/IF, Mouse) ICC/IF Mouse
Fishbein I, Guerrero DT, Alferiev IS, Foster JB. Stent-based delivery of adeno-associated viral vectors with sustained vascular transduction and iNOS-mediated inhibition of in-stent restenosis. Gene Ther. 2017 Nov 01 [PMID: 28832561] (IHC, Rat) IHC Rat
Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 2017 Jan [PMID: 28190929]
Kalliokoski S, Piqueras VO, Frias R et al. Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice. Amino Acids. Aug 9 2016 [PMID: 27503559] (IHC, Mouse) IHC Mouse
Liang X, Luo M, Wei XW et al. A folate receptor-targeted lipoplex delivering interleukin-15 gene for colon cancer immunotherapy. Oncotarget. Jul 11 2016 [PMID: 27438147] (IHC) IHC
Yancu D, Blouin MJ, Birman E et al. Insulin-like growth factor-3 knockout mice are resistant to experimental dextran sulfate sodium colitis. J. Gastroenterol. Hepatol. Jun 2 2016 [PMID: 27253188] (IHC) IHC
Show All 37 Publications.

Reviews for Ki-67/MKI67 Antibody (NB110-89717) (7) 4.67

Average Rating: 4.6
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB110-89717:
Filter by Applications
Flow
(2)
IF
(1)
IHC-P
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(4)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 06/16/2017
View

Summary

ApplicationFlow Cytometry
Sample TestedBlood mononuclear cells (PBMCs)
SpeciesHuman
Immunofluorescence Ki-67/MKI67 NB110-89717
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reviewed by:
Anonymous
IF Rat 01/06/2016
View

Summary

ApplicationImmunofluorescence
SpeciesRat
Lot030209
FileView PDF
Immunohistochemistry-Paraffin Ki-67/MKI67 NB110-89717
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reviewed by:
Alexandra Ambrico
IHC-P Mouse 06/16/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse prostate
SpeciesMouse
 Ki-67/MKI67 NB110-89717
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reviewed by:
Anonymous
Mouse 04/28/2015
View

Summary

SpeciesMouse
FileView PDF
Immunohistochemistry-Paraffin Ki-67/MKI67 NB110-89717
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reviewed by:
Ramakumar Tummala
IHC-P Human 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Xenografts
SpeciesHuman
Lot050806-1
 
reviewed by:
Anonymous
IHC-P Mouse 12/08/2011
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Liver Tissue
SpeciesMouse

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Rat HRP-Polymer (DAKO)

Details

Detection NotesDetection Method: DAB (HRP)
Flow Cytometry Ki-67/MKI67 NB110-89717
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reviewed by:
Huiyuan Zhang
Flow Mouse 07/14/2010
View

Summary

ApplicationFlow Cytometry
Sample Testedmouse bone marrow cells
SpeciesMouse
CommentsI tried your antibody for Flow staining. I used the common intracellular staining protocol and dilution of Ki67 is 1:100.

Comments

CommentsI tried your antibody for Flow staining. I used the common intracellular staining protocol and dilution of Ki67 is 1:100.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ki-67/MKI67 Antibody (NB110-89717). (Showing 1 - 2 of 2 FAQs).

  1. We have a customer who is using NB110-89717. She has seen the statement on one of the IHC images that says, Detection: DAB staining using Immunohistochemistry Accessory Kit. Epitope Retrieval Buffer-High pH was substituted for Epitope Retrieval Buffer-Reduced pH. She would like to know if you recommend Tris buffer pH 10 or if not what you do recommend for the best results. Any info you have is much appreciated.
    • Yes, the customer may use a Tris pH 10 buffer if that is what she wish to and we believe it should work fine. If she don't get the expected results we would recommend switching to a citrate buffer pH 6.0 as this is what our lab typically tests our products with.
  2. About the Ki-67/MKI67 antibody NB110-89717,does it contain BSA as a carrier protein?
    • Our product NB110-89717 is provided in PBS pH 7.4 with 0.05% sodium azide as preservative. The buffer does not contain any BSA.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NB110-89717V
FITC Labeled NB110-89717F
PE Labeled NB110-89717PE

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Bioinformatics Tool for Ki-67/MKI67 Antibody (NB110-89717)

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Blogs on Ki-67/MKI67.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Ki67 - an established marker for labelling proliferating cells
Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v...  Read full blog post.

Ki67 - A Crucial Cellular Proliferation Marker
The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line....  Read full blog post.

The Ki67 Antibody in Cell Marker Studies
The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is...  Read full blog post.

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Recent Reviews

4.6
5
4
4
3
3
0
2
0
1
0

 
Anonymous
06/16/2017
Application: Flow
Species: Human

Anonymous
01/06/2016
Application: IF
Species: Rat

Alexandra Ambrico
06/16/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol MKI67
Entrez
Uniprot