Ki-67/MKI67 Antibody (SP6)

Images

 
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody (SP6) [NB600-1252] - Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody.
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody (SP6) [NB600-1252] - Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clone
SP6
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Ki-67/MKI67 Antibody (SP6) Summary

Immunogen
A synthetic peptide from C-terminus of human Ki-67.
Epitope
C-terminus
Localization
Nuclear
Marker
Proliferation Marker
Specificity
Ki67 (SP6)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MKI67
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
Suggested incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 20235278) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23300752)
Positive Control
Tonsil Lysate (NB820-59272)
Tonsil normal tissue Slide (S152W0)
Reviewed Applications
Read 1 Review rated 5
using
NB600-1252 in the following applications:

Publications
Read Publications using
NB600-1252 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22020958). Reactivity of Ki-67 antibody' clone SP6 with Canine/Dog species is reported in PMIDs 23618385, 21982272 and 19490419.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and BSA
Preservative
0.05% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Ki-67/MKI67 Antibody (SP6)

  • antigen identified by monoclonal Ki-67
  • antigen KI-67
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-1
  • MKI67
  • PPP1R105
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Background

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Ki-67/MKI67 Antibody (NB600-1252)(29)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(2)
IHC
(4)
IHC-Fr
(2)
IHC-P
(12)
All Applications
Filter By Species
Human
(8)
Mouse
(7)
Rat
(4)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB600-1252 Applications Species
Penna I, Gigoni A, Costa D et al. The inhibition of 45A ncRNA expression reduces tumor formation, affecting tumor nodules compactness and metastatic potential in neuroblastoma cells. Oncotarget. Jan 31 2017 [PMID: 28029658] (IHC, Mouse) IHC Mouse
Sumis A, Cook K, Andrade FO et al. Social isolation induces autophagy in the mouse mammary gland: link to increased mammary cancer risk. Endocr. Relat. Cancer. Aug 22 2016 [PMID: 27550962] (IHC, Mouse) IHC Mouse
Kalliokoski S, Piqueras VO, Frias R et al. Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice. Amino Acids. Aug 9 2016 [PMID: 27503559] (IHC, Mouse) IHC Mouse
Rath SK, Deb M, Sengupta D et al. Silencing of ZRF1 impedes survival of estrogen receptor positive MCF-7 cells and potentiates the effect of curcumin Tumour Biol. 2016 Jun 27 [PMID: 27350366] (IHC, Human) IHC Human
Ueha R, Ueha S, Sakamoto T et al. Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice. Neurotox Res 2016 Aug [PMID: 27003941]
Migita H, Yoshitake S, Tange Y et al. Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular Regeneration After Renal Ischemia/Reperfusion Injury in Rats. Nephrourol Mon 2016 Jan [PMID: 26981502]
Ueha R, Ueha S, Kondo K et al. Damage to Olfactory Progenitor Cells Is Involved in Cigarette Smoke-Induced Olfactory Dysfunction in Mice. Am J Pathol 2016 Mar [PMID: 26806086]
Falkenberg N, Hofig I, Rosemann M et al. Three-dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer. Cancer Med. 2016 Jan 14 [PMID: 26763588] (IHC-P, Human) IHC-P Human
Du Z, Bondarenko O, Wang D et al. Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression. J. Cell. Physiol. 2015 Oct 30 [PMID: 26516696] (IHC-P, Rat) IHC-P Rat
Vesci L, Carollo V, Roscilli G et al. [Corrigendum] Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance. Oncol Rep 2016 Jan [PMID: 26499228]
Show All 29 Publications.

Review for Ki-67/MKI67 Antibody (NB600-1252) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NB600-1252:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Ki-67/MKI67 NB600-1252
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reviewed by:
Feng Li
ICC Mouse 05/30/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedthyroid tumor
SpeciesMouse
LotG746

Product General Protocols

View specific protocols for Ki-67/MKI67 Antibody (NB600-1252): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Ki-67/MKI67 Antibody (NB600-1252). (Showing 1 - 2 of 2 FAQs).

  1. I have bought via kristalindo biolab (Indonesia) primary antibody ki-67 (catalog # NB600-1252). Do you have a positive control for that ? My sample is breast cancer from rat.
    • In our lab, we have tested this product in tonsil tissues and we recommend either using a tonsil tissue from rat or human as a positive control to determine that the staining you see in your rat breast cancer tissue is specific. We do have a tonsil slide that you might find useful (catalog # S152W0).
  2. I am using the Ki-67 antibody (NB600-1252) and I need some information about the protein concentration.
    • Please note that this product is a "Tissue Culture Supernatant" production and the exact concentration of the antibody is not quantifiable in it. Technically speaking, the tissue culture supernatants can have the IgG contents from 1 - 3 mg/ml.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Ki-67/MKI67 Products

Bioinformatics Tool for Ki-67/MKI67 Antibody (NB600-1252)

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Blogs on Ki-67/MKI67.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Ki67 - an established marker for labelling proliferating cells
Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v...  Read full blog post.

Ki67 - A Crucial Cellular Proliferation Marker
The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line....  Read full blog post.

The Ki67 Antibody in Cell Marker Studies
The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is...  Read full blog post.

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Recent Reviews

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Feng Li
05/30/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol MKI67
Entrez