Ki-67/MKI67 Antibody (SP6)


Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody (SP6) [NB600-1252] - Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody.

Product Details

Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Ki-67/MKI67 Antibody (SP6) Summary

A synthetic peptide from C-terminus of human Ki-67.
Proliferation Marker
Ki67 (SP6)
Tissue culture supernatant
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.05% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.
Tissue culture supernatant


Application Notes
Suggested incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 20235278) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23300752)
Read Publications using
NB600-1252 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22020958). Reactivity of Ki-67 antibody' clone SP6 with Canine/Dog species is reported in PMIDs 23618385, 21982272 and 19490419.

Alternate Names for Ki-67/MKI67 Antibody (SP6)

  • antigen identified by monoclonal Ki-67
  • antigen KI-67
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-1
  • MKI67
  • PPP1R105
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126


Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: ICC/IF, IHC, IHC-P
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Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
Species: Hu, Mu, Rt, Ca
Applications: ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Ki-67/MKI67 Antibody (NB600-1252)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.

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Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB600-1252 Applications Species
Rath SK, Deb M, Sengupta D et al. Silencing of ZRF1 impedes survival of estrogen receptor positive MCF-7 cells and potentiates the effect of curcumin Tumour Biol. 2016 Jun 27 [PMID:27350366] (IHC, Human) IHC Human
Falkenberg N, Hofig I, Rosemann M et al. Three-dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer. Cancer Med. 2016 Jan 14 [PMID:26763588] (IHC-P, Human) IHC-P Human
Du Z, Bondarenko O, Wang D et al. Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression. J. Cell. Physiol. 2015 Oct 30 [PMID:26516696] (IHC-P, Rat) IHC-P Rat
Vesci L, Carollo V, Roscilli G et al. [Corrigendum] Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance. Oncol Rep 2016 Jan [PMID:26499228]
Vesci L, Carollo V, Roscilli G et al. Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance Oncol. Rep. 2015 Jun 24 [PMID:26133490] (IHC-P, Human) IHC-P Human
Tukler Henriksson J, Coursey TG, Corry DB et al. IL-13 Stimulates Proliferation and Expression of Mucin and Immunomodulatory Genes in Cultured Conjunctival Goblet Cells. Invest Ophthalmol Vis Sci 2015 Jul [PMID:26132778] (ICC/IF, Mouse) ICC/IF Mouse
Kim LA, Wong LL, Amarnani DS et al. Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy Mol. Vis. 2015 Jun 29 [PMID:26120272] (IHC-P, Human) IHC-P Human
Cage TA, Chanthery Y, Chesler L. Downregulation of MYCN through PI3K inhibition in mouse models of pediatric neural cancer Front. Oncol. 2015 Apr 27 [PMID:26029667] (IHC-P, Human) IHC-P Human
Schevzov G, Kee AJ, Wang B et al. Regulation of cell proliferation by ERK and signal-dependent nuclear translocation of ERK is dependent on Tm5NM1-containing actin filaments. Mol. Biol. Cell. 2015 May 13 [PMID:25971798] (IHC-P, Mouse) IHC-P Mouse
Kalliokoski S, Caja S, Frias R et al. Injection of celiac disease patient sera or immunoglobulins to mice reproduces a condition mimicking early developing celiac disease. J Mol. Med. 2014 Sep 12 [PMID:25209899]
Show All 23 Publications.

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Product General Protocols

View specific protocols for Ki-67/MKI67 Antibody (NB600-1252): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Ki-67/MKI67 Antibody (NB600-1252). (Showing 1 - 2 of 2 FAQs).

  1. I have bought via kristalindo biolab (Indonesia) primary antibody ki-67 (catalog # NB600-1252). Do you have a positive control for that ? My sample is breast cancer from rat.
    • In our lab, we have tested this product in tonsil tissues and we recommend either using a tonsil tissue from rat or human as a positive control to determine that the staining you see in your rat breast cancer tissue is specific. We do have a tonsil slide that you might find useful (catalog # S152W0).
  2. I am using the Ki-67 antibody (NB600-1252) and I need some information about the protein concentration.
    • Please note that this product is a "Tissue Culture Supernatant" production and the exact concentration of the antibody is not quantifiable in it. Technically speaking, the tissue culture supernatants can have the IgG contents from 1 - 3 mg/ml.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol MKI67

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