Suggested incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 20235278) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23300752)
Mouse reactivity reported in scientific literature (PMID: 22020958). Reactivity of Ki-67 antibody' clone SP6 with Canine/Dog species is reported in PMIDs 23618385, 21982272 and 19490419.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS and BSA
0.05% Sodium Azide
Tissue culture supernatant
Alternate Names for Ki-67/MKI67 Antibody (SP6)
antigen identified by monoclonal Ki-67
Marker Of Proliferation Ki-67
proliferation-related Ki-67 antigen
Protein Phosphatase 1
Regulatory Subunit 105
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Ki-67/MKI67 Antibody (NB600-1252). (Showing 1 - 2 of 2 FAQs).
I have bought via kristalindo biolab (Indonesia) primary antibody ki-67 (catalog # NB600-1252). Do you have a positive control for that ? My sample is breast cancer from rat.
In our lab, we have tested this product in tonsil tissues and we recommend either using a tonsil tissue from rat or human as a positive control to determine that the staining you see in your rat breast cancer tissue is specific. We do have a tonsil slide that you might find useful (catalog # S152W0).
I am using the Ki-67 antibody (NB600-1252) and I need some information about the protein concentration.
Please note that this product is a "Tissue Culture Supernatant" production and the exact concentration of the antibody is not quantifiable in it. Technically speaking, the tissue culture supernatants can have the IgG contents from 1 - 3 mg/ml.
The Ki67 Antibody in Cell Marker Studies The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is... Read full blog post.
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