KAT4/TBP Associated Factor 1 Antibody

Images

 
Western Blot: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and ...read more
Immunohistochemistry: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - Tissue: prostate. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-TAF1 at 10 ug/mL for 1 h at RT. Secondary antibody: ...read more
Chromatin Immunoprecipitation: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] - Chromatin Immunoprecipitation: KAT4/TBP Associated Factor 1 Antibody [NBP1-77847] Transcription Initiation Factor TFIID Subunit 1 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ELISA, IHC, IHC-P, IP, NULL
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

KAT4/TBP Associated Factor 1 Antibody Summary

Immunogen
This affinity purified KAT4/TBP Associated Factor 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human KAT4/TBP Associated Factor 1. (Uniprot: P21675)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA 1:150000-1:300000
  • Immunohistochemistry 10ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • N/A
  • Western Blot 1:100-1:2000
Application Notes
Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 213 kDa in size corresponding to KAT4/TBP Associated Factor 1 protein by western blotting in the appropriate cell lysate or extract.
Theoretical MW
212.68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with KAT4/TBP Associated Factor 1 protein from rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This affinity purified antibody is directed against human KAT4/TBP Associated Factor 1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for KAT4/TBP Associated Factor 1 Antibody

  • 250kDa
  • BA2Rdystonia 3 (with Parkinsonism)
  • CCG1TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 250kD
  • CCGS
  • Cell cycle gene 1 protein
  • cell cycle, G1 phase defect
  • complementation of cell cycle block, G1-to-S
  • DYT3/TAF1
  • EC 2.7.11
  • EC 2.7.11.1
  • KAT4
  • NSCL2
  • OF
  • P250
  • TAF(II)250
  • TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor
  • TAF2AN-TAF1
  • TAFII-250
  • TAFII250DYT3
  • TBP-associated factor 250 kDa
  • transcription factor TFIID p250 polypeptide
  • Transcription initiation factor TFIID 250 kDa subunit
  • transcription initiation factor TFIID subunit 1
  • XDP

Background

Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors. TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II. TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53. TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1). The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity. TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18% of mammalian genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TAF1
Uniprot