RAP74 Antibody (OTI4B10)

Images

 
Western Blot: RAP74 Antibody (OTI4B10) [NBP3-17971] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RAP74 monoclonal antibody.
Immunohistochemistry-Paraffin: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RAP74 mouse monoclonal antibody.
Flow Cytometry: RAP74 Antibody (OTI4B10) [NBP3-17971] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAP74 antibody, and then ...read more
Western Blot: RAP74 Antibody (OTI4B10) [NBP3-17971] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAP74 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RAP74 mouse monoclonal antibody.
Immunohistochemistry: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Human endometrium tissue using anti-RAP74 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
OTI4B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.44 mg/ml

Order Details

RAP74 Antibody (OTI4B10) Summary

Immunogen
Full length human recombinant protein of human GTF2F1(NP_002087) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GTF2F1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.44 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAP74 Antibody (OTI4B10)

  • BTF4
  • General transcription factor IIF 74 kDa subunit
  • general transcription factor IIF subunit 1
  • general transcription factor IIF, polypeptide 1, 74kDa
  • TF2F1
  • TFIIF
  • TFIIF-alpha
  • Transcription initiation factor IIF subunit alpha
  • transcription initiation factor RAP74

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GTF2F1