KAP1 Antibody - BSA Free

Western Blot: KAP1 Antibody [NB500-158] - Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-158] - FFPE sections of human breast carcinoma. KAP1 antibody Lot3 (left) and Lot2 (right) used at a dilution of 1:1000 (1 ug/mL). Detection: DAB.

Western Blot: KAP1 Antibody [NB500-158] - Detection of Human KAP1 by Western blot. Whole cell lysate from HeLa (5, 15 and 50 ug), and 293T (50 ug) cells. KAP1 antibody at 0.1 ug/mL. Detection by chemiluminescence with an exposure time of 30 seconds.

Western Blot: KAP1 Antibody [NB500-158] - Hep3B human hepatocellular carcinoma cell line. 50 ug cell lysate per lane. 10% SDS-PAGE. KAP1 (TRIM28) antibody at 1:1000 dilution in 5% milk at 4C O/N. WB image submitted by a verified customer review.

Immunoprecipitation: KAP1 Antibody [NB500-158] - Detection of human KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NB500-158 (lot NB500-158-3) used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-158-2) and rabbit anti-KAP-1 antibody NB500-159. For blotting immunoprecipitated KAP-1, NB500-158 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

KAP1 Antibody - BSA Free Summary

• Description: This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-2350, NB100-2241
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM28
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:500- 1:2500
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 ug/mg of lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618) Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. KAP1 antibody validated for WB from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-2350, NB100-2241

Alternate Names for KAP1 Antibody - BSA Free

• E3 SUMO-protein ligase TRIM28
• EC 6.3.2.-
• FLJ29029
• KAP1
• KAP-1
• KAP1KRAB-associated protein 1
• KRIP-1
• Nuclear corepressor KAP-1
• RNF96
• RNF96KRAB-interacting protein 1
• TF1B
• TIF1B
• TIF1-beta
• TIF1BRING finger protein 96
• transcription intermediary factor 1-beta
• transcriptional intermediary factor 1-beta
• TRIM28
• tripartite motif containing 28
• tripartite motif-containing 28
• Tripartite motif-containing protein 28

Background

The nuclear corepressor, KAP-1, is involved in silencing heterochromatin. It has also been suggested to play a role in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KAP1 Antibody (NB500-158)(19)

Publications using NB500-158

• Takagi M, Hoshino A, Bousset K et al. Bone Marrow Failure and Immunodeficiency Associated with Human RAD50 Variants Journal of clinical immunology 2023-10-05 [PMID: 37794136]
• Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications 2022-01-14 [PMID: 35031618] (IP, Chemotaxis, WB)
• Chang J, Hwang HJ, Kim B et al. TRIM28 functions as a negative regulator of aggresome formation Autophagy 2021-04-12 [PMID: 33783327]
• Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015-03-27 [PMID: 25820262] (WB, Mouse)
• A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200C>A with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity. Nakamura K, Fike F, Haghayegh S Mol Genet Genomic Med 2014-02-02 [PMID: 25077176] (WB, Human)
• Puccini J, Shalini S, Voss AK et al. Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice. Proc Natl Acad Sci U S A 2013-12-03 [PMID: 24248351] (WB, Mouse)
• Yang Y, Fiskus W, Yong B et al. Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy. Proc Natl Acad Sci U S A 2013-04-23 [PMID: 23569248] (WB, ICC/IF, IP, Human)
• Nayler S, Gatei M, Kozlov S et al. Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells: Induced Pluripotent Stem Cells from Ataxia-Telangiectasia Recapitulate the Cellular Phenotype Stem Cells Transl Med 2012-12-01 [PMID: 23197857]
• Bhatia N, Xiao TZ, Rosenthal KA et al. MAGE-C2 Promotes Growth and Tumorigenicity of Melanoma Cells, Phosphorylation of KAP1, and DNA Damage Repair J Invest Dermatol 2012-10-25 [PMID: 23096706] (WB, Human)
• Gatei M, Jakob B, Chen P et al. ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control J Biol Chem 2011-09-09 [PMID: 21757780] (WB, Human)
• Bhatia N, Yang B, Xiao TZ et al. Identification of novel small molecules that inhibit protein-protein interactions between MAGE and KAP-1 Arch Biochem Biophys 2011-04-15 [PMID: 21277283] (IP, Human)
• Narayan V, Halada P, Hernychova L et al. A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1. J Biol Chem 2011-04-01 [PMID: 21245151]
• Gilmore-Hebert M, Ramabhadran R, Stern DF et al. Interactions of ErbB4 and Kap1 connect the growth factor and DNA damage response pathways. Mol Cancer Res 2010-10-01 [PMID: 20858735]
• Briers S, Crawford C, Bickmore WA et al. KRAB zinc-finger proteins localise to novel KAP1-containing foci that are adjacent to PML nuclear bodies. J Cell Sci 2009-04-01 [PMID: 19258395]
• Tsuruma R, Ohbayashi N, Kamitani S et al. Physical and functional interactions between STAT3 and KAP1. Oncogene 2008-05-01 [PMID: 18037959]
• Yang, B et al. EXPERIMENTAL THERAPEUTICS, MOLECULAR TARGETS, CHEMICAL BIOLOGY: MAGE-A, mMage-b, MAGE-C Proteins Form Complexes with KAP1 Suppress p53-Dependent Apoptosis in MAGE-Positive Cell Lines. Cancer Res 67:9954-9962. 2007-10-01 [PMID: 17942928]
• Wang C, Rauscher FJ 3rd, Cress WD et al. Regulation of E2F1 function by the nuclear corepressor KAP1. J Biol Chem 2007-10-01 [PMID: 17704056]
• Ziv Y, Bielopolski D, Galanty Y et al. Chromatin relaxation in response to DNA double-strand breaks is modulated by a novel ATM- and KAP-1 dependent pathway. Nat Cell Biol 2006-08-01 [PMID: 16862143]
• Wang C, Ivanov A, Chen L et al. MDM2 interaction with nuclear corepressor KAP1 contributes to p53 inactivation. EMBO J 2005-09-01 [PMID: 16107876]

Review for KAP1 Antibody (NB500-158) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Yongkang Yang WB Human 08/16/2019

Summary

• Application: Western Blot
• Sample Tested: Hep3B human hepatocellular carcinoma cell line
• Species: Human
• Lot: A3

Comments

• Comments: 50ug Hep3b cells lysate/lane, 10% SDS-PAGE; Primary antibody (TRIM28 Antibody (NB500-158)): 1:1000 diluted in 5% milk incubate at 4℃ for overnight.

Bioinformatics

• Gene Symbol: TRIM28
• Entrez:
  • 10155(Human)
• Uniprot:
  • Q13263(Human)