KAP1 Antibody

Images

 
Western Blot: KAP1 Antibody [NB500-158] - Hep3B human hepatocellular carcinoma cell line. 50 ug cell lysate per lane. 10% SDS-PAGE. KAP1 (TRIM28) antibody at 1:1000 dilution in 5% milk at 4C O/N. WB image submitted by a ...read more
Immunohistochemistry: KAP1 Antibody [NB500-158] - FFPE sections of human breast carcinoma. KAP1 antibody Lot3 (left) and Lot2 (right) used at a dilution of 1:1000 (1 ug/mL). Detection: DAB.
Biological Strategies: Western Blot: KAP1 Antibody [NB500-158] - Whole cell lysate from HeLa (5, 15, 50 ug), and 293T (50ug) cells.
Biological Strategies: Western Blot: KAP1 Antibody [NB500-158] - Detection of Human KAP1 by Western blot. Whole cell lysate from HeLa (5, 15 and 50 ug), and 293T (50 ug) cells. KAP1 antibody at 0.1 ug/mL. ...read more
Independent Antibodies: Immunoprecipitation: KAP1 Antibody [NB500-158] - Detection of human KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

KAP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:500- 1:2500
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 ug/mg of lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunocytochemistry 1:500 to 1:2,500. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Control
KAP1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB500-158 in the following applications:

Publications
Read Publications using
NB500-158 in the following applications:

  • 1 publication
  • IP
    2 publications
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-2350, NB100-2241

Alternate Names for KAP1 Antibody

  • E3 SUMO-protein ligase TRIM28
  • EC 6.3.2.-
  • FLJ29029
  • KAP1
  • KAP-1
  • KAP1KRAB-associated protein 1
  • KRIP-1
  • Nuclear corepressor KAP-1
  • RNF96
  • RNF96KRAB-interacting protein 1
  • TF1B
  • TIF1B
  • TIF1-beta
  • TIF1BRING finger protein 96
  • transcription intermediary factor 1-beta
  • transcriptional intermediary factor 1-beta
  • TRIM28
  • tripartite motif containing 28
  • tripartite motif-containing 28
  • Tripartite motif-containing protein 28

Background

The nuclear corepressor, KAP-1, is involved in silencing heterochromatin. It has also been suggested to play a role in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB500-158
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for KAP1 Antibody (NB500-158)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(2)
WB
(7)
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Human
(6)
Mouse
(2)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB500-158 Applications Species
Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015 Mar 27 [PMID: 25820262] (WB, Mouse) WB Mouse
A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200C>A with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity. Nakamura K, Fike F, Haghayegh S Mol Genet Genomic Med Feb 2 2014 [PMID: 25077176] (WB, Human) WB Human
Puccini J, Shalini S, Voss AK et al. Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice. Proc Natl Acad Sci U S A 2013 Dec 3 [PMID: 24248351] (WB, Mouse) WB Mouse
Yang Y, Fiskus W, Yong B et al. Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy. Proc Natl Acad Sci U S A 2013 Apr 23 [PMID: 23569248] (WB, ICC/IF, IP, Human) WB, ICC/IF, IP Human
Bhatia N, Xiao TZ, Rosenthal KA et al. MAGE-C2 Promotes Growth and Tumorigenicity of Melanoma Cells, Phosphorylation of KAP1, and DNA Damage Repair J Invest Dermatol 2012 Oct 25 [PMID: 23096706] (WB, Human) WB Human
Gatei M, Jakob B, Chen P et al. ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control J Biol Chem 2011 Sep 9 [PMID: 21757780] (WB, Human) WB Human
Bhatia N, Yang B, Xiao TZ et al. Identification of novel small molecules that inhibit protein-protein interactions between MAGE and KAP-1. Arch Biochem Biophys. Apr. 2011 [PMID: 21277283] (IP, Human) IP Human
Narayan V, Halada P, Hernychova L et al. A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1. J Biol Chem 2011 Apr [PMID: 21245151]
Gilmore-Hebert M, Ramabhadran R, Stern DF et al. Interactions of ErbB4 and Kap1 connect the growth factor and DNA damage response pathways. Mol Cancer Res 2010 Oct [PMID: 20858735]
Briers S, Crawford C, Bickmore WA et al. KRAB zinc-finger proteins localise to novel KAP1-containing foci that are adjacent to PML nuclear bodies. J Cell Sci 2009 Apr [PMID: 19258395]
Show All 16 Publications.

Review for KAP1 Antibody (NB500-158) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB500-158:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot KAP1 NB500-158
Enlarge
5
reviewed by:
Yongkang Yang
WB Human 08/16/2019
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Summary

ApplicationWestern Blot
Sample TestedHep3B human hepatocellular carcinoma cell line
SpeciesHuman
LotA3

Comments

Comments50ug Hep3b cells lysate/lane, 10% SDS-PAGE; Primary antibody (TRIM28 Antibody (NB500-158)): 1:1000 diluted in 5% milk incubate at 4℃ for overnight.

Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Recent Reviews

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1
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Yongkang Yang
08/16/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol TRIM28
Entrez
Uniprot