KAP1 Antibody

Images

 
Western Blot: KAP1 Antibody [NB500-158] - Detection of Human KAP-1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), and 293T (50ug) cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody ...read more
Immunohistochemistry: KAP1 Antibody [NB500-158] - Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-KAP-1 Cat. No. Lot3 (left) and Lot2 (right) used at a dilution of 1:1,000 ...read more
Western Blot: KAP1 Antibody [NB500-158] - Whole cell lysate from HeLa (5, 15, 50 ug), and 293T (50ug) cells.
Western Blot: KAP1 Antibody [NB500-158] - Whole cell lysate from TCMK-1 (50ug) cells.
Western Blot: KAP1 Antibody [NB500-158] - Detection of Human KAP-1 on HeLa whole cell lysate using NB500-158. Immunoprecipitated KAP-1 was detected using a KAP-1 antibody from another source.
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-158] - FFPE section of human skin tumor. Affinity purified rabbit anti-KAP-1 used at a dilution of 1:100.
Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-158] - Human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right). Antibody used at a dilution of 1:1,000. (left) and green fluorescent goat ...read more
Proximity Ligation Assay: KAP1 Antibody [NB500-158] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human KAP1 (NB500-158). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: KAP1 Antibody [NB500-158] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human KAP1 (NB500-158). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: KAP1 Antibody [NB500-158] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HP1-beta (NB100-79774) and rabbit anti-human KAP1 (NB500-158). Image merged from DAPI (2ms) and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

KAP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:1000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunocytochemistry 1:500 to 1:2,500. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Publications
Read Publications using
NB500-158 in the following applications:

  • 1 publication
  • IP
    2 publications
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-2350, NB100-2241

Alternate Names for KAP1 Antibody

  • E3 SUMO-protein ligase TRIM28
  • EC 6.3.2.-
  • FLJ29029
  • KAP1
  • KAP-1
  • KAP1KRAB-associated protein 1
  • KRIP-1
  • Nuclear corepressor KAP-1
  • RNF96
  • RNF96KRAB-interacting protein 1
  • TF1B
  • TIF1B
  • TIF1-beta
  • TIF1BRING finger protein 96
  • transcription intermediary factor 1-beta
  • transcriptional intermediary factor 1-beta
  • TRIM28
  • tripartite motif containing 28
  • tripartite motif-containing 28
  • Tripartite motif-containing protein 28

Background

The nuclear corepressor, KAP-1, is involved in silencing heterochromatin. It has also been suggested to play a role in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
NB100-2596
Species: Hu, Mu
Applications: WB, ChIP, IHC-P, IP, PLA
NB110-40623
Species: Hu
Applications: WB, ChIP, IHC, IHC-P, IP, PLA
NBP2-27562
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-20322
Species: Hu
Applications: WB, ChIP, IP
NB100-56340
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
NBP1-80608
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-384
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-304
Species: Hu, Mu, Rt, Fi, Gt, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
NB500-171
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
NB100-59787
Species: Hu, Mu
Applications: WB, IB, ICC/IF, IHC, IHC-P, IP, PLA
NBP1-82639
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-49156
Species: Hu
Applications: ICC/IF, IHC
NB500-158
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, PLA

Publications for KAP1 Antibody (NB500-158)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(2)
WB
(7)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB500-158 Applications Species
Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015 Mar 27 [PMID: 25820262] (WB, Mouse) WB Mouse
Puccini J, Shalini S, Voss AK et al. Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice. Proc Natl Acad Sci U S A 2013 Dec 3 [PMID: 24248351] (WB, Mouse) WB Mouse
Yang Y, Fiskus W, Yong B et al. Acetylated hsp70 and KAP1-mediated Vps34 SUMOylation is required for autophagosome creation in autophagy. Proc Natl Acad Sci U S A 2013 Apr 23 [PMID: 23569248] (WB, ICC/IF, IP, Human) WB, ICC/IF, IP Human
Bhatia N, Xiao TZ, Rosenthal KA et al. MAGE-C2 Promotes Growth and Tumorigenicity of Melanoma Cells, Phosphorylation of KAP1, and DNA Damage Repair J Invest Dermatol 2012 Oct 25 [PMID: 23096706] (WB, Human) WB Human
Gatei M, Jakob B, Chen P et al. ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control J Biol Chem 2011 Sep 9 [PMID: 21757780] (WB, Human) WB Human
Bhatia N, Yang B, Xiao TZ et al. Identification of novel small molecules that inhibit protein-protein interactions between MAGE and KAP-1. Arch Biochem Biophys. Apr. 2011 [PMID: 21277283] (IP, Human) IP Human
Narayan V, Halada P, Hernychova L et al. A multiprotein binding interface in an intrinsically disordered region of the tumor suppressor protein interferon regulatory factor-1. J Biol Chem 2011 Apr [PMID: 21245151]
Gilmore-Hebert M, Ramabhadran R, Stern DF et al. Interactions of ErbB4 and Kap1 connect the growth factor and DNA damage response pathways. Mol Cancer Res 2010 Oct [PMID: 20858735]
Briers S, Crawford C, Bickmore WA et al. KRAB zinc-finger proteins localise to novel KAP1-containing foci that are adjacent to PML nuclear bodies. J Cell Sci 2009 Apr [PMID: 19258395]
Tsuruma R, Ohbayashi N, Kamitani S et al. Physical and functional interactions between STAT3 and KAP1. Oncogene 2008 May [PMID: 18037959]
Show All 16 Publications.

Reviews for KAP1 Antibody (NB500-158) (0)

There are no reviews for KAP1 Antibody (NB500-158). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KAP1 Antibody (NB500-158) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional KAP1 Products

Bioinformatics Tool for KAP1 Antibody (NB500-158)

Discover related pathways, diseases and genes to KAP1 Antibody (NB500-158). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for KAP1 Antibody (NB500-158)

Discover more about diseases related to KAP1 Antibody (NB500-158).
 

Pathways for KAP1 Antibody (NB500-158)

View related products by pathway.

PTMs for KAP1 Antibody (NB500-158)

Learn more about PTMs related to KAP1 Antibody (NB500-158).
 

Research Areas for KAP1 Antibody (NB500-158)

Find related products by research area.

Blogs on KAP1

There are no specific blogs for KAP1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our KAP1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TRIM28
Entrez
Uniprot