KAP1 Antibody (BL-248-2G6)

Western Blot: KAP1 Antibody (BL-248-2G6) [NBP2-76411] - Detection of human and mouse KAP-1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411). Secondary: HRP-conjugated goat anti-rabbit IgG.

Immunocytochemistry: KAP1 Antibody (BL-248-2G6) [NBP2-76411] - Detection of human KAP-1 in FFPE HeLa cells by ICC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry: KAP1 Antibody (BL-248-2G6) [NBP2-76411] - Detection of human KAP-1 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunoprecipitation: KAP1 Antibody (BL-248-2G6) [NBP2-76411] - Detection of human KAP-1 by WB of immunoprecipitates from HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411) and rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398).

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: BL-248-2G6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

KAP1 Antibody (BL-248-2G6) Summary

• Description: Novus Biologicals Rabbit KAP1 Antibody (BL-248-2G6) (NBP2-76411) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Surrounding serine 824
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TRIM28
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 µl per 1 X 10^6 cells
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for KAP1 Antibody (BL-248-2G6)

• E3 SUMO-protein ligase TRIM28
• EC 6.3.2.-
• FLJ29029
• KAP1
• KAP-1
• KAP1KRAB-associated protein 1
• KRIP-1
• Nuclear corepressor KAP-1
• RNF96
• RNF96KRAB-interacting protein 1
• TF1B
• TIF1B
• TIF1-beta
• TIF1BRING finger protein 96
• transcription intermediary factor 1-beta
• transcriptional intermediary factor 1-beta
• TRIM28
• tripartite motif containing 28
• tripartite motif-containing 28
• Tripartite motif-containing protein 28

Background

KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM28