KAP1 [p Ser824] Antibody (BL-246-7B5)

Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.

Western Blot: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by WB of HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Lower panel shows WB for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG.

Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP1 (S824) in FFPE breast carcinoma by IHC and in FFPE EPE treated HeLa cells by ICC. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) (red) in formaldehyde-fixed asynchronous HeLa cells treated with EPE (right) or untreated (left) by ICC-IF. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin conjugated Alexafluor 488 (green).

Immunoprecipitation: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phopsho KAP-1 (S824) by WB of immunoprecipitates from HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398) and rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411).

Flow Cytometry- KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Antibody: Rabbitanti-phospho KAP-1 recombinant monoclonal[BL-246-7B5] or isotype control (unshaded).Secondary: DyLight® 488-conjugated goat anti-rabbit IgG

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP
• Clone: BL-246-7B5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

KAP1 [p Ser824] Antibody (BL-246-7B5) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155)
• Modification: p Ser824
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TRIM28
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 20 ul/mg lysate
  • Simple Western 1:10 - 1:250
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for KAP1 [p Ser824] Antibody (BL-246-7B5)

• E3 SUMO-protein ligase TRIM28
• EC 6.3.2.-
• FLJ29029
• KAP1
• KAP-1
• KAP1KRAB-associated protein 1
• KRIP-1
• Nuclear corepressor KAP-1
• RNF96
• RNF96KRAB-interacting protein 1
• TF1B
• TIF1B
• TIF1-beta
• TIF1BRING finger protein 96
• transcription intermediary factor 1-beta
• transcriptional intermediary factor 1-beta
• TRIM28
• tripartite motif containing 28
• tripartite motif-containing 28
• Tripartite motif-containing protein 28

Background

KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM28