HP1 alpha Antibody

Images

 
Western Blot: HP1 alpha Antibody [NB100-2518] - Detection of HP 1 alpha in HeLa whole cell lysate using NB100-2518.
Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody [NB100-2518] - HP1 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight ...read more
Flow (Intracellular): HP1 alpha Antibody [NB100-2518] - Analysis using the Alexa Fluor 488 conjugate of NB100-2518. Detection of HeLa cells (1 x 10^6 cells/mL) with HP-1 antibody (orange) stained at 1:500. Shown with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

HP1 alpha Antibody Summary

Immunogen
A synthetic peptide containing an N-terminal region of human HP1 alpha (within residues 1-100). [Swiss-Prot P45973]
Localization
Nuclear
Marker
Heterochromatin Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBX5
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular) 1:500
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:500 - 1:1000
Application Notes
In Western blot a band is seen at ~20 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
HP1 alpha Overexpression Lysate

Reactivity Notes

Expected to react with Mouse, Arabidopsis suecica, Rye and Triticum aestivum due to sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
IgG purified

Alternate Names for HP1 alpha Antibody

  • Antigen p25
  • chromobox homolog 5 (Drosophila HP1 alpha)
  • chromobox homolog 5 (HP1 alpha homolog, Drosophila)
  • chromobox homolog 5
  • chromobox protein homolog 5
  • Heterochromatin protein 1 homolog alpha
  • heterochromatin protein 1-alpha
  • HP1 alpha homolog
  • HP1 alpha
  • HP1
  • HP1A
  • HP1-ALPHA
  • HP1Hs alpha
  • HP1Hs-alpha

Background

HP1 alpha (heterochromatin protein 1 homolog alpha or CBX5) is a component of heterochromatin that recognizes and binds H3K9me (histone H3 tails methylated at 'Lys-9') for facilitating epigenetic repression and is excluded from chromatin once histone H3 is phosphorylated at Tyr 41 position (H3Y41ph). HP1 alpha can interact with LBR (lamin-B receptor) thereby mediating heterochromatin association with inner nuclear membrane and its interaction with MIS12 complex proteins is implicated in functional kinetochore formation. HP1 alpha is a highly conserved nonhistone protein with chromatin organization modifier domain or chromodomain, and is localized in the nucleus where it participates in centromeric/pericentromeric heterochromatin and associates with chromosomes during mitotic metaphase/anaphase facilitating accurate cell division. It also serve as a common gene expression signature shared by human mature oocytes and embryonic stem cells, and through interactions with ATRX as well as H3.3, HP1 alpha acts a key regulator of ES-cell telomere chromatin. HP1 alpha is up-regulated in breast cancer cells and in lung cancer, it is essential to the maintenance of aggressiveness as well as stem cell like properties of tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HP1 alpha Antibody (NB100-2518) (0)

There are no publications for HP1 alpha Antibody (NB100-2518).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HP1 alpha Antibody (NB100-2518) (0)

There are no reviews for HP1 alpha Antibody (NB100-2518). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for HP1 alpha Antibody (NB100-2518): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HP1 alpha Antibody (NB100-2518) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HP1 alpha Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CBX5