HP1 alpha Antibody


Western Blot: HP1 alpha Antibody [NB100-2518] - Detection of HP 1 alpha in HeLa whole cell lysate using NB100-2518.
Immunocytochemistry/ Immunofluorescence: HP1 alpha Antibody [NB100-2518] - HP1 alpha antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight ...read more
Flow (Intracellular): HP1 alpha Antibody [NB100-2518] - Analysis using the Alexa Fluor 488 conjugate of NB100-2518. Detection of HeLa cells (1 x 10^6 cells/mL) with HP-1 antibody (orange) stained at 1:500. Shown with ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF

Order Details

HP1 alpha Antibody Summary

A synthetic peptide containing an N-terminal region of human HP1 alpha (within residues 1-100). [Swiss-Prot P45973]
Heterochromatin Marker
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow (Intracellular) 1:500
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:500 - 1:1000
Application Notes
In Western blot a band is seen at ~20 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
HeLa Whole Cell Lysate
HP1 alpha Overexpression Lysate

Reactivity Notes

Expected to react with Mouse, Arabidopsis suecica, Rye and Triticum aestivum due to sequence homology.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
IgG purified

Alternate Names for HP1 alpha Antibody

  • Antigen p25
  • chromobox homolog 5 (Drosophila HP1 alpha)
  • chromobox homolog 5 (HP1 alpha homolog, Drosophila)
  • chromobox homolog 5
  • chromobox protein homolog 5
  • Heterochromatin protein 1 homolog alpha
  • heterochromatin protein 1-alpha
  • HP1 alpha homolog
  • HP1 alpha
  • HP1
  • HP1A
  • HP1Hs alpha
  • HP1Hs-alpha


HP1 alpha (heterochromatin protein 1 homolog alpha or CBX5) is a component of heterochromatin that recognizes and binds H3K9me (histone H3 tails methylated at 'Lys-9') for facilitating epigenetic repression and is excluded from chromatin once histone H3 is phosphorylated at Tyr 41 position (H3Y41ph). HP1 alpha can interact with LBR (lamin-B receptor) thereby mediating heterochromatin association with inner nuclear membrane and its interaction with MIS12 complex proteins is implicated in functional kinetochore formation. HP1 alpha is a highly conserved nonhistone protein with chromatin organization modifier domain or chromodomain, and is localized in the nucleus where it participates in centromeric/pericentromeric heterochromatin and associates with chromosomes during mitotic metaphase/anaphase facilitating accurate cell division. It also serve as a common gene expression signature shared by human mature oocytes and embryonic stem cells, and through interactions with ATRX as well as H3.3, HP1 alpha acts a key regulator of ES-cell telomere chromatin. HP1 alpha is up-regulated in breast cancer cells and in lung cancer, it is essential to the maintenance of aggressiveness as well as stem cell like properties of tumor cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for HP1 alpha Antibody (NB100-2518): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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mFluor Violet Conjugated Antibodies

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Gene Symbol CBX5