JAMP Antibody - BSA Free

Western Blot: JAMP Antibody [NBP2-36446] - Western blot analysis of JAMP in HEK293 and A431 cell lysates.

Immunocytochemistry/Immunofluorescence: JAMP Antibody [NBP2-36446] - JAMP antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Endoplasmic reticulum staining was observed.

Immunohistochemistry-Paraffin: JAMP Antibody [NBP2-36446] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal kidney using rabbit polyclonal JAMP antibody (NBP2-36446) at 10 ug/ml concentration. The cells of various tubules/ducts showed a weak to moderate cytoplasmic immunopositivity for JAMP protein.

Immunohistochemistry-Paraffin: JAMP Antibody [NBP2-36446] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human hepatocellular carcinoma using rabbit polyclonal JAMP antibody (NBP2-36446) at 10 ug/ml concentration. Majority of the cancer cells depicted the expected cytoplasmic staining expression pattern.

Immunohistochemistry-Paraffin: JAMP Antibody [NBP2-36446] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human placenta using rabbit polyclonal JAMP antibody (NBP2-36446) at 10 ug/ml concentration. A cytoplasmic staining expression pattern was seen in the syncytiotrophoblast and cytiotrophoblasts of chorionic villi capillaries.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

JAMP Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human JAMP protein (between amino acids 20-150) [UniProt Q9P055]
• Localization: Endoplasmic Reticulum
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: JKAMP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 2 ug/ml
• Control:

  293 Whole Cell Lysate
  A-431 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for JAMP Antibody - BSA Free

• C14orf100
• chromosome 14 open reading frame 100
• HSPC213
• HSPC327
• JAMPCDA06
• JNK1/MAPK8-associated membrane protein
• JNK1-associated membrane protein
• JNK-associated membrane protein
• Jun N-terminal kinase 1-associated membrane protein
• Medulloblastoma antigen MU-MB-50.4

Background

JAMP, better known as the JNK1/MAPK8 associated membrane protein, is a regulator of MAPK8 activity in response to various stress stimuli. This regulation is part of a broader response to ER stress which induces JAMP to facilitate the degradation of misfolded ER luminal proteins via recruitment of the ERAD (endoplasmic reticulum-associated degradation system). Aside from interactions with MAPK8, JAMP also interacts with RNF5, as well as many regulatory proteins in the ERAD such as AMFR/GP78, CANX, PSMC1/PSMC2 and PSMC5-8. JAMP is expressed in many tissues, including brain, spleen, thymus, liver, kidney and testis. It also exhibits elevated expression in medulloblastomas. JAMP undergoes a post-translational modification after ubiquitination by RNF5 in a UBE2N-dependent manner, which ultimately decreases association with the proteasome and ERAD. Research has confirmed that JAMP regulates JNK-1 activity (PMID: 16166642) as well as optimizes ERAD to protect cells from unfolded proteins (PMID 18784250) which may have various implications in disease models.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JAMP Antibody (NBP2-36446)(1)

Publication using NBP2-36446

• Peng, S, Shi, S Et al. JKAMP inhibits the osteogenic capacity of adipose-derived stem cells in diabetic osteoporosis by modulating the Wnt signaling pathway through intragenic DNA methylation. Stem Cell Res Ther 2021-02-12 [PMID: 33579371] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: JKAMP
• Entrez:
  • 51528(Human)
• Uniprot:
  • Q9P055(Human)