JNK2 Antibody (252320) [Unconjugated]

Images

 
JNK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal Antibody (Catalog # MAB1846) at 25 µg/mL for 3 hours at room temperature. Cells ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal Antibody (Catalog ...read more
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and JNK2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, KO
Clone
252320
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

JNK2 Antibody (252320) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human JNK2 isoform 2
Specificity
Detects human, mouse, and rat JNK2, expressed as p46 JNK (isoforms 2 and/or 3, both 382 aa) and p54 JNK (isoforms 1 and/or 4, both 424 aa) in Western blots. Does not detect recombinant JNK1 or JNK3.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MAPK9
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
Reviewed Applications
Read 2 Reviews rated 5
using
MAB1846 in the following applications:

Publications
Read Publications using
MAB1846 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JNK2 Antibody (252320) [Unconjugated]

  • c-Jun kinase 2
  • c-Jun N-terminal kinase 2
  • EC 2.7.11
  • EC 2.7.11.24
  • JNK2
  • JNK2A
  • JNK2BETA
  • JNK2JNK2ALPHA
  • JNK-55
  • Jun kinase
  • MAP kinase 9
  • MAPK 9
  • MAPK9
  • mitogen-activated protein kinase 9
  • p54a
  • p54aSAPK
  • PRKM9JNK2B
  • SAPK
  • SAPK1 alpha
  • Stress-activated protein kinase JNK2

Background

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-37568
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-68874
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
NBP2-00764
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP1-88419
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-81575
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-47839
Species: Hu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, ICC
AF1347
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
H00005706-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, RNAi

Publications for JNK2 Antibody (MAB1846)(2)

Reviews for JNK2 Antibody (MAB1846) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat and Mouse.

Reviews using MAB1846:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Rat and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot JNK2 MAB1846
Enlarge
reviewed by:
Anonymous
WB Rat and Mouse 12/31/2016
View

Summary

ApplicationWestern Blot
Sample TestedRetina
SpeciesRat and Mouse

Comments

CommentsJNK2 Ab was used at 1:2000 over night, followed by peroxidase conjugated donkey anti mouse secondary antibody and chemiluminescence detection.
Western Blot JNK2 MAB1846
Enlarge
reviewed by:
Anonymous
WB Human 10/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:2000, 2 hours at RT

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:3000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for JNK2 Antibody (MAB1846) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional JNK2 Products

Bioinformatics Tool for JNK2 Antibody (MAB1846)

Discover related pathways, diseases and genes to JNK2 Antibody (MAB1846). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for JNK2 Antibody (MAB1846)

Discover more about diseases related to JNK2 Antibody (MAB1846).
 

Pathways for JNK2 Antibody (MAB1846)

View related products by pathway.

PTMs for JNK2 Antibody (MAB1846)

Learn more about PTMs related to JNK2 Antibody (MAB1846).

Blogs on JNK2

There are no specific blogs for JNK2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
12/31/2016
Application: WB
Species: Rat and Mouse

Anonymous
10/04/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAPK9
Entrez